Articles tagged "Mixed chimerism"

2019 American Transplant Congress
Prolongation of Allograft Survival via Donor MHC Chimerism Induced by Exosomes
B. Gonzalez Nolasco¹, M. Wang¹, W. Orent¹, A. Prunevieille¹, J. O¹, K. Ahrens¹, J. C. Madsen², G. Benichou¹
¹Department of Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Department of Surgery, Center for Transplantation Sciences, Division of Cardiac Surgery, Massachusetts General Hospital, Boston, MA
*Purpose: Achieving robust and durable host immune tolerance of allogeneic transplants is the ultimate goal in clinical transplantation. Mixed chimerism induced via donor bone marrow…
2019 American Transplant Congress
Expansion of Regulatory T and B Cells in Swine Rendered Tolerant of Allogeneic Vascularized Composite Allografts via Mixed Chimerism
G. Saviane¹, A. Lellouch¹, A. Andrews¹, L. Lantieri², M. Randolph¹, G. Benichou¹, C. Cetrulo Jr¹
¹Center for Transplantation Sciences, Division of Plastic Surgery, Boston, MA, USA, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA, Boston, MA, ²European Georges Pompidou Hospital, University of Paris, Department of Plastic Surgery, Paris, France
*Purpose: Prevention of acute rejection of vascularized composite allografts (VCAs) requires ongoing systemic immunosuppression, which subject patients to infectious, metabolic, reno-vascular and neoplastic complications. It…
2019 American Transplant Congress
Significantly High FOXP3 mRNA in the Renal Allograft as a Mechanism of Tolerance Induced by Transient Mixed Chimerism in Non-Human Primates
M. Matsunami¹, I. Rosales¹, T. Oura¹, B. Adam², M. Mengel², R. Smith¹, A. Cosimi¹, R. Colvin¹, T. Kawai¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA, ²Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
*Purpose: Treg-rich organized lymphoid structures (TOLS) has been identified in recipients with renal allograft tolerance induced by transient mixed chimerism in non-human primates (NHPs) and…
2018 American Transplant Congress
Enhancement of Intrinsic Apoptosis via Bcl-2 Inhibition Promotes Induction of Robust Mixed Chimerism and Renal Allograft Tolerance without Myelosuppression in Nonhuman Primates
T. Oura,¹ A. Dehnadi,¹ I. Rosales,¹ H. Sasaki,¹ P. Cippa,² R. Fehr,² A. Cosimi,¹ T. Kawai.¹
¹Surgery, Center for Transplantation Science, Massachusetts General Hospital, Boston, MA; ²Medicine, University of Zurich, Zurich, Switzerland.
Background: Successful induction of mixed chimerism (MC) and renal allograft tolerance has been achieved in both nonhuman primate (NHP) and man following conditioning that included…
2018 American Transplant Congress
Robust Hematopoietic Mixed Chimerism and Donor-Specific Skin Allograft Tolerance after Non-Genotoxic Anti-CD117 Immunotoxin Conditioning and Donor Bone Marrow Allotransplantation
Z. Li,¹ A. Czechowicz,^2,3,4,5,6,7 A. Scheck,^2,3,4,5,6 D. Rossi,^3,4,5,6 P. Murphy.¹
¹Lab of Molecular Immunology (LMI), NIAID, Bethesda; ²Dept Pediatrics, Div Stem Cell Transplantation and Regenerative Med, Stanford Univ, Stanford; ³Pgram Cellular & Molecular Med, Div Hematology/Oncology, Boston Children's Hosp, Boston; ⁴Dept Stem Cell & Regenerative Biol, Harvard Univ, Boston; ⁵Dept Pediatrics, Harvard Med Sch, Boston; ⁶Harvard Stem Cell Inst, Harvard Univ, Cambridge; ⁷Dept Pediatric Oncology, Dana Farber Cancer Inst, Boston.
Establishing hematopoietic chimerism enables donor-specific allograft tolerance; however, chimerism protocols typically require either genotoxic conditioning or mega-doses of donor bone marrow cells (BMC), impeding clinical…
2018 American Transplant Congress
PD-L1 May Serve as a Biomarker for Chimerism-Induced Tolerance in Kidney Transplant Recipients
A. Merchak,¹ A. Khalil,¹ A. Chhabra,¹ J. Leventhal,² S. Ildstad.¹
¹University of Louisville, Louisville, KY; ²Northwestern Memorial Hospital, Chicago, IL.
Durable chimerism is currently the most reliable biomarker of donor specific tolerance. Identifying and validating other predictive biomarkers is therefore a high priority. Since 2009…
2018 American Transplant Congress
Non-Myeloablative Conditioning with Low-Dose Total Body Irradiation in Place of Cyclophosphamide Induces Mixed Chimerism and Long-Term Immunosuppression Free Allograft Survival without Acute Kidney Injury in HLA Mismatched Kidney Transplantation
T. Kawai,¹ T. Spitzer,¹ T. Oura,¹ N. Torkoff-Rubin,¹ N. Elias,¹ M. Sykes,² R. Colvin,¹ D. Sachs,² A. Cosimi.¹
¹Transplant Center, Massachusetts General Hospital, Boston, MA; ²Center for Translational Immunology, Columbia University, New York, NY.
We have achieved successful induction of long-term immunosuppression (IS) free renal allograft survival in HLA mismatched kidney transplantation using cyclophosphamide (CP) based conditioning and combined…
2018 American Transplant Congress
Immunity and Tolerance after Bilateral Orthotopic Lung Transplant (BOLT) in Tandem with a CD3+/CD19+ Depleted Vertebral Bone Marrow Transplant (BOLT+BMT) from 1 of 8 HLA-Matched Cadaveric Donors
P. Szabolcs,¹ X. Chen,¹ A. Donnenberg,² M. Hill,¹ J. Rowan,¹ S. Mcintyre,¹ G. Kurland,¹ J. Barnum,¹ J. McDyer.¹
¹BMT-CT, Children Hospital of Pittsburgh UPMC, Pittsburgh, PA; ²IDM-HCC, University of Pittsburgh, Pittsburgh, PA.
Primary immunodeficiency patients may develop pulmonary complications and most are ineligible for either lung transplant or BMT due to futility. We report our first 2…
2018 American Transplant Congress
Absolute Quantification of Plasma Graft-Derived Cell-Free DNA for Diagnosis of Kidney Transplant Rejection
J. Whitlam,¹ L. Ling,² F. Ierino,³ D. Bruno,² H. Slater,² D. Power.¹
¹Austin Health, Heidelberg, Victoria, Australia; ²Murdoch Childrens Research Institute, Parkville, Victoria, Australia; ³St Vincent's Health, Fitzroy, Victoria, Australia.
Aim: To study the performance of droplet digital PCR absolute measurement of graft-derived cell-free DNA (gdcfDNA) for the diagnosis of kidney allograft rejection.Methods: Homozygous deletion…
2018 American Transplant Congress
A Novel Conditioning Approach to Establish Chimerism Using Manipulation of JAK-STAT Pathways
H. Xu, A. Merchak, L. Kahn, A. Chhabra, Y. Wen, Y. Huang, S. Ildstad.
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY.
Hematopoietic chimerism establishes donor-specific tolerance to solid organ allografts. Targeting recipient immune cells (T, NK cells and macrophages) can effectively reduce the dose of total…