Articles tagged "Mixed chimerism"

2019 American Transplant Congress
Desensitization Using Costimulatory Blockade and Bortezomib Does Not Prevent DSA Formation and Rejection in Reconstructive Transplantation
F. Messner¹, Y. Guo¹, R. Chen², Y. Kuo², G. Brandacher¹, B. Oh¹
¹Department of Plastic and Reconstructive Surgery, Johns Hopkins UJohns Hopkins University School of Medicine, Baltimore, MD, ²Division of Plastic Surgery, Kaohsiung Medical Univerity Hospital, Kaohsiung, Taiwan
*Purpose: Sensitiziation in form of donor-specific antibodies (DSA) and subsequent antibody-mediated rejection (AMR) constitute some of the greatest risk factors for allograft rejection and long-term…
2019 American Transplant Congress
A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Lymphocyte Biology and Transplant Immunity
L. Qiu¹, J. Wang², R. Fernandez³, X. Yeap¹, S. Han¹, F. Zheng¹, C. Lin², Z. J. Zhang¹
¹Comprehensive Transplant Center, Department of Surgery, Northwestern University, Chicago, IL, ²Northwestern University, Chicago, IL, ³Department of Surgery, Northwestern University, Chicago, IL
*Purpose: Spleen-derived lymphocytes and myeloid cell populations are critical to the development of organ transplant ischemia-reperfusion injury (IRI) and allograft rejection. In this study, we…
2019 American Transplant Congress
Full and Sustained Donor Hematopoietic Chimerism after an Isolated Kidney Transplantation
J. Zuber¹, O. Boyer¹, B. Neven¹, E. Six², R. Berthaud¹, M. Ould Rabah¹, S. Sobrino², C. Lagresle², I. André-Schmutz², M. Cavazzana¹, S. Blanche¹, R. Salomon¹
¹Paris Descartes University, Paris, France, ²Inserm UMRS 1163, Paris, France
*Purpose: Full donor chimerism was reported three times in the absence of myelosuppressive regimen, only after liver or multivisceral transplantation, but never after kidney transplantation.…
2018 American Transplant Congress
Immunity and Tolerance after Bilateral Orthotopic Lung Transplant (BOLT) in Tandem with a CD3+/CD19+ Depleted Vertebral Bone Marrow Transplant (BOLT+BMT) from 1 of 8 HLA-Matched Cadaveric Donors
P. Szabolcs,¹ X. Chen,¹ A. Donnenberg,² M. Hill,¹ J. Rowan,¹ S. Mcintyre,¹ G. Kurland,¹ J. Barnum,¹ J. McDyer.¹
¹BMT-CT, Children Hospital of Pittsburgh UPMC, Pittsburgh, PA; ²IDM-HCC, University of Pittsburgh, Pittsburgh, PA.
Primary immunodeficiency patients may develop pulmonary complications and most are ineligible for either lung transplant or BMT due to futility. We report our first 2…
2018 American Transplant Congress
Absolute Quantification of Plasma Graft-Derived Cell-Free DNA for Diagnosis of Kidney Transplant Rejection
J. Whitlam,¹ L. Ling,² F. Ierino,³ D. Bruno,² H. Slater,² D. Power.¹
¹Austin Health, Heidelberg, Victoria, Australia; ²Murdoch Childrens Research Institute, Parkville, Victoria, Australia; ³St Vincent's Health, Fitzroy, Victoria, Australia.
Aim: To study the performance of droplet digital PCR absolute measurement of graft-derived cell-free DNA (gdcfDNA) for the diagnosis of kidney allograft rejection.Methods: Homozygous deletion…
2018 American Transplant Congress
A Novel Conditioning Approach to Establish Chimerism Using Manipulation of JAK-STAT Pathways
H. Xu, A. Merchak, L. Kahn, A. Chhabra, Y. Wen, Y. Huang, S. Ildstad.
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY.
Hematopoietic chimerism establishes donor-specific tolerance to solid organ allografts. Targeting recipient immune cells (T, NK cells and macrophages) can effectively reduce the dose of total…
2018 American Transplant Congress
Tomotherapy and Donor Hematopoietic Cells for Tolerance to Kidney Transplants in Rhesus Macaques
D. Kaufman,¹ L. Haynes,¹ P. Hematti,¹ W. Burlingham,¹ L. Forest,² J. Post,¹ R. Redfield,¹ L. Fernandez,¹ A. D'Alessandro,¹ J. Haynes,¹ K. Jensen,³ S. Strober.³
¹Surgery, University of Wisconsin School of Medicine, Madison, WI; ²Surgical Sciences, University of Wisconsin School of Vet Med, Madison, WI; ³Medicine, Stanford University Medical School, Palo Alto, CA.
The goal of this project was to develop a tolerance induction protocol for MHC 1-haplotype mismatched kidney transplants (txs) in a rhesus macaque model. The…
2018 American Transplant Congress
Immunomodulation of Alloantibody Responses
C. Schuetz, M. Climov, A. Paril, Z. Wang, A. Andrews Roy, N. Navarro Alvarez, A. Matar, D. Sachs, R. Duran-Struuck, C. Huang.
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA.
We have shown that i.v. exposure to MHC-mismatched donor PBMC using a novel, mild conditioning protocol results in B-cell tolerance with transient T-cell unresponsiveness. Here…
2018 American Transplant Congress
Predicting Outcomes of Rat Vascularized Composite Allotransplants through Quantitative Measurement of Chimerism with PCR-Amplified Short Tandem Repeat
H. Cheng,¹ X. Huang,² C-.F. Lin,¹ N. AL Deek,² L. Shih,² C-.H. Lin,^1,2 F-.C. Wei.^1,2,3
¹Center for Vascularized Composite Allotransplantation, Linkou Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; ²Department of Plastic and Reconstructive Surgery, Linkou Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; ³College of Medicine, Chang Gung University, Gueishan, Taoyuan, Taiwan.
Background: Chimerism, defined as coexistence of donor and recipient cells, has been associated with induction and maintenance of tolerance to allotransplants. Although most studies have…
2018 American Transplant Congress
CD8-Expressing Transitional B Cells Dominate B Cell Reconstitution in Transplanted Monkeys Receiving Mixed Chimerism Conditioning
K. Pruner, J. Paster, S. Wiebke, O. Jane, H. Isabel, D. Abbas, B. Gilles, M. Joren.
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA.
IntroductionTolerance of heart or lung allografts have been achieved for the first time in nonhuman primates (NHPs) using a mixed chimerism conditioning regimen. However, the…