Articles tagged "Machine preservation"

2019 American Transplant Congress
Defatting Steatotic Rat Livers during Ex Situ Normothermic Perfusion Improves Lactate Clearance and Bile Quality
S. Raigani¹, C. Carroll², S. Cronin², C. Pendexter², I. Rosales³, M. Yarmush¹, K. Uygun¹, H. Yeh¹
¹Transplant Surgery, Massachusetts General Hospital, Boston, MA, ²Center for Engineering in Medicine, Shriners Childrens Hospital, Boston, MA, ³Pathology, Massachusetts General Hospital, Boston, MA
*Purpose: Donor livers with moderate-to-severe steatosis are not generally used for transplantation because of poor post-transplant function. Ex situ normothermic machine perfusion (ENMP) provides an…
2019 American Transplant Congress
Viability Testing and Transplantation of Marginal Donor Livers (VITTAL) Trial Outcomes: Proteomic Analysis of Perfusates from Livers Undergoing Normothermic Machine Liver Perfusion Reveals Biomarkers Predictive of Graft Viability and Post-Transplant Complications
S. Afford¹, L. Wallace¹, J. Attard¹, Y. Boteon¹, R. Laing¹, J. Yu², T. Perera³, D. Mirza⁴, H. Mergental⁵
¹Centre for Liver and Gastroenterology Research, University of Birmingham, Birmingham, United Kingdom, ²The School of Biosciences Proteomics Facility, University of Birmingham, Birmingham, United Kingdom, ³Liver Unit, Queen Elizabeth University Hospital, Birmingham, United Kingdom, ⁴Liver Unit, The Queen Elizabeth University Hospital, Birmingham, United Kingdom, ⁵The Liver Unit, Queen Elizabeth University Hospital, Birmingham, United Kingdom
*Purpose: The VITTAL clinical trial demonstrated that post-ischaemic normothermic machine perfusion(NMP) can increase utilisation of livers from the marginal pool of extended criteria donors (ECD).…
2019 American Transplant Congress
Total Parenteral Nutrition in Ex Vivo Lung Perfusion: Addressing Metabolism Improves Both Inflammation and Overall Graft Performance
M. T. Buchko, S. Hatami, C. J. Stewart, S. Himmat, D. H. Freed, J. Nagendran
Department of Surgery, Division of Cardiac Surgery, University of Alberta, Edmonton, AB, Canada
*Purpose: Contemporary clinical ex vivo lung perfusion (EVLP) protocols generally limit metabolic supplementation to insulin and glucose. Furthermore, conventional perfusates are void of many ingredients…
2019 American Transplant Congress
Pharmacological Enhancement of Bile Duct Preservation and Function during Ex-Situ Normothermic Machine Perfusion
Y. L. Boteon, J. Attard, A. P. Boteon, L. Wallace, G. Reynolds, D. Mirza, H. Mergental, R. Bhogal, S. Afford
Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, United Kingdom
*Purpose: Whilst ex-situ normothermic machine perfusion (NMP) of the liver limits the ischaemic injury to the organ, benefits for biliary complications post-transplantation are not yet…
2019 American Transplant Congress
A Novel Multi-Thermic, Antiseptic, and Anthropomorphic System for Ex-Vivo Face Perfusion
J. W. Etra¹, J. Brassil², F. A. Chang¹, S. A. Fidder¹, D. S. Cooney¹, G. Brandacher¹
¹Johns Hopkins University School of Medicine, Baltimore, MD, ²Functional Circulation LLC, Northbrook, IL
*Purpose: Facial disfigurement is profoundly disabling, and great relief follows resolution by facial transplantation, now a technical reality at multiple centers worldwide. However, distance between…
2019 American Transplant Congress
Normothermic Ex-Situ Liver Perfusion Parameters of Liver Viability in a Porcine Model of Liver Transplantation with Marginal Grafts
I. Linares-Cervantes¹, J. Echeverri¹, S. Cleland¹, M. Kaths¹, R. Rosales¹, T. Goto¹, D. Kollmann¹, M. Hamar¹, P. Urbanellis¹, L. Mazilescu¹, S. Ganesh¹, O. Adeyi¹, P. Yip¹, B. Bojko², P. Gorynzka², K. Gorynski², D. Grant¹, N. Selzner¹, M. Wasowicz¹, M. Selzner¹
¹Toronto General Hospital, Toronto, ON, Canada, ²University of Nicolaus Copernicus, Torun, Poland
*Purpose: Evaluate parameters of liver viability during normothermic ex-situ liver perfusion (NEsLP) prior to transplantation.*Methods: In this study, we investigated prior assessed parameters and new…
2019 American Transplant Congress
Optimizing a Protocol for Extended Ex-Vivo Extremity Perfusion
C. J. Stewart¹, M. T. Buchko², X. Wang¹, G. Brandacher³, A. Ladak⁴, D. H. Freed², J. Nagendran²
¹Surgery, University of Alberta, Edmonton, AB, Canada, ²Cardiac Surgery, University of Alberta, Edmonton, AB, Canada, ³Plastic and Reconstruction Surgery, Johns Hopkins University, Baltimore, MD, ⁴Plastic Surgery, University of Alberta, Edmonton, AB, Canada
*Purpose: Our objective is to demonstrate that Ex-Vivo Extremity Perfusion (EVEP) of a Vascularized Composite Allograft (VCA) is as effective, or superior, to standard cold…
2019 American Transplant Congress
Near-Infrared Fluorescence Imaging with ZW800-1 Dye to Assess Donor Kidneys While on Ex-Vivo Normothermic Machine Perfusion
A. S. Arykbaeva¹, K. M. Rozenberg², M. L. Lo Faro², J. Hunter², J. B. Doppenberg¹, A. R. Valentijn³, A. G. Terwisscha van Scheltinga³, H. Putter⁴, I. P. Alwayn¹, J. V. Frangioni⁵, J. Burggraaf⁶, A. L. Vahrmeijer¹, R. J. Ploeg², V. A. Huurman¹
¹Department of Surgery, Leiden University Medical Center, Leiden, Netherlands, ²Nuffield Department of Surgical Sciences, Oxford, United Kingdom, ³Department of Clinical Pharmacy and Toxicology, Leiden University Medical Center, Leiden, Netherlands, ⁴Department of Biomedical Data Sciences, Leiden University Medical Center, Leiden, Netherlands, ⁵Curadel, LCC, Marlborough, MA, ⁶Centre for Human Drug Research, Leiden, Netherlands
*Purpose: At present, more older and higher risk donor kidneys are accepted resulting in increased uncertainty about quality. This may result in unjustified discard of…
2019 American Transplant Congress
Alpha-1 Antitrypsin Reduces Liver Ischemia Reperfusion Injury during Ex-Vivo Normothermic Machine Perfusion
D. Detelich¹, R. de Vries¹, C. Carroll¹, M. Stein², K. Uygun¹, H. Yeh¹, J. Markmann¹
¹Massachusetts General Hospital, Boston, MA, ²Kamada, Rehovot, Israel
*Purpose: Normothermic machine perfusion is an emerging preservation method in liver transplantation with vast potential as a platform for ex-vivo graft reconditioning. Alpha-1 Antitrypsin (AAT)…
2019 American Transplant Congress
Ex-vivo Xenoperfusion Of Wild Type And Galtko.hcd55 Pig Livers With Human Whole Blood
D. Detelich, C. Rickert, C. Eymard, C. Carroll, S. Kimura, H. Yeh, J. Markmann
Massachusetts General Hospital, Boston, MA
*Purpose: Pig-to-human liver xenotransplantation is not currently feasible due to rapid and profound thrombocytopenia, coagulopathy and hyperacute rejection. Successful development of genetically modified swine to…