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Articles tagged "Machine preservation"

  • 2019 American Transplant Congress

    Sirna Uptake During Ex Vivo Hypothermic And Normothermic Liver Machine Perfusion: Beginning Of A New Era Of Organ Preservation

    A. Gillooly1, C. Moore1, J. Perry1, I. Bruggenwirth2, M. Thijssen2, X. Wang1, X. E1, P. Mandrekar1, T. Kowalik1, R. Porte2, H. Leuvenink2, R. Abdi3, A. Bozorgzadeh1, P. N. Martins1

    1University of Massachusetts Medical School, Worcester, MA, 2University of Groningen, Groningen, Netherlands, 3Brigham and Women's Hospital, Boston, MA

    *Purpose: RNA interference (RNAi) is a natural gene modulation process that was recently approved for clinical use and has great potential for treatment of many…
  • 2019 American Transplant Congress

    Normothermic Machine Perfusion of Human Kidneys without an Oxygen Carrier

    D. Detelich, C. Eymard, M. Aburawi, C. Carroll, K. Uygun, H. Yeh, J. F. Markmann

    Massachusetts General Hospital, Boston, MA

    *Purpose: Normothermic machine perfusion (NMP) is a promising strategy for pre-transplant reconditioning and viability assessment of marginal renal grafts. All current clinical kidney NMP protocols…
  • 2019 American Transplant Congress

    Brief Normothermic Machine Perfusion is Superior to Cold Storage Alone – A Paired Discarded Human Kidney Analysis

    A. Hameed1, E. Patrick2, D. B. Lu1, C. P'ng1, M. Hu2, Y. Chew2, R. De Roo1, L. Yuen1, W. Hawthorne1, N. Rogers1, H. Pleass1

    1Westmead Hospital, Sydney, Australia, 2Westmead Institute for Medical Research, Sydney, Australia

    *Purpose: The use of marginal has increased due to ongoing donor shortages. These kidneys have higher rates of graft discard after procurement, and also have…
  • 2019 American Transplant Congress

    CD47 Blockade Promotes Hepatic Macrophage Polarization toward M1 during Normothermic Machine Perfusion of Discarded Human Livers

    M. Xu1, F. Zhou1, S. Garcia-Aroz1, B. Banan1, W. Yang2, G. Upadhya1, K. Byrnes3, B. Wong1, P. Manning4, Y. Lin1, W. Chapman1

    1Department of Surgery, Washington University School of Medicine, St. Louis, MO, 2Department of Genetics, Washington University School of Medicine, St. Louis, MO, 3Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 4Arch Oncology, St. Louis, MO, USA, St. Louis, MO

    *Purpose: M1 macrophages have been shown to be able to phagocytize dead cells after ischemia-reperfusion injury (IRI). This study investigated the therapeutic effect of CD47…
  • 2019 American Transplant Congress

    Machine Perfusion Allows Use of High KDPI and Prolonged Cold Ischemia Time in Deceased Donors: A Single Center Effort to Decrease Discard Rates

    M. Ortigosa-Goggins, J. Gaynor, A. Mattiazzi, J. Figueiro, G. Burke, G. Ciancio, G. Guerra

    Miami Transplant Institute, University of Miami, Miami, FL

    *Purpose: Discard rates of deceased donor kidney transplants(DDKT)rises as the kidney donor profile index(KDPI)increases,and approaches 60%for KDPI of >85%.Pulsatile machine perfusion(MP)allows use of kidneys with…
  • 2019 American Transplant Congress

    Metabolic Profiling of Steatotic Human Livers during Ex-Vivo Normothermic Machine Perfusion

    S. Raigani1, N. Karimian2, V. Huang2, S. Geerts2, S. Nagpal2, K. Uygun1, M. Yarmush1, H. Yeh1

    1Transplant Surgery, Massachusetts General Hospital, Boston, MA, 2Center for Engineering in Medicine, Shriners Childrens Hospital, Boston, MA

    *Purpose: Nearly 40-60% of discarded livers are due to steatosis because of poor recovery from ischemia-reperfusion injury and compromised post-transplant function. We evaluated steatotic (ST)…
  • 2019 American Transplant Congress

    Elevated Podocyte-specific Urinary Extracellular Vesicles In Patients With Fsgs Recurrence After Kidney Transplantation

    H. Lau, A. Shimomura, E. Tantisattamo, U. G. Reddy, D. Dafoe, H. Ichii

    University of California Irvine, Orange, CA

    *Purpose: Kidney graft failure is a major concern in patients with kidney transplantation. Patients who suffered graft failure are at a higher risk for mortality…
  • 2019 American Transplant Congress

    Ischemia-Free Liver Transplantation Protects Bile Duct from Ischemia/Reperfusion Injury

    J. Yang, S. Huang, Z. Zhang, Q. Zhao, W. Ju, Z. Zhu, Y. Tang, L. Wang, Y. Zhang, C. Sun, L. Wu, Z. Guo, X. He

    Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

    *Purpose: Ischemia-free liver transplantation (IFLT), based on normothermic machine perfusion and innovations in surgical technique, can provide continue blood supply from procurement to implantation that…
  • 2019 American Transplant Congress

    Defatting Steatotic Rat Livers during Ex Situ Normothermic Perfusion Improves Lactate Clearance and Bile Quality

    S. Raigani1, C. Carroll2, S. Cronin2, C. Pendexter2, I. Rosales3, M. Yarmush1, K. Uygun1, H. Yeh1

    1Transplant Surgery, Massachusetts General Hospital, Boston, MA, 2Center for Engineering in Medicine, Shriners Childrens Hospital, Boston, MA, 3Pathology, Massachusetts General Hospital, Boston, MA

    *Purpose: Donor livers with moderate-to-severe steatosis are not generally used for transplantation because of poor post-transplant function. Ex situ normothermic machine perfusion (ENMP) provides an…
  • 2019 American Transplant Congress

    Viability Testing and Transplantation of Marginal Donor Livers (VITTAL) Trial Outcomes: Proteomic Analysis of Perfusates from Livers Undergoing Normothermic Machine Liver Perfusion Reveals Biomarkers Predictive of Graft Viability and Post-Transplant Complications

    S. Afford1, L. Wallace1, J. Attard1, Y. Boteon1, R. Laing1, J. Yu2, T. Perera3, D. Mirza4, H. Mergental5

    1Centre for Liver and Gastroenterology Research, University of Birmingham, Birmingham, United Kingdom, 2The School of Biosciences Proteomics Facility, University of Birmingham, Birmingham, United Kingdom, 3Liver Unit, Queen Elizabeth University Hospital, Birmingham, United Kingdom, 4Liver Unit, The Queen Elizabeth University Hospital, Birmingham, United Kingdom, 5The Liver Unit, Queen Elizabeth University Hospital, Birmingham, United Kingdom

    *Purpose: The VITTAL clinical trial demonstrated that post-ischaemic normothermic machine perfusion(NMP) can increase utilisation of livers from the marginal pool of extended criteria donors (ECD).…
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