Articles tagged "Liver preservation"

2021 American Transplant Congress
Cross-Circulation for Extracorporeal Liver Support in a Swine Model
W. K. Wu¹, A. Tumen¹, J. W. Stokes², R. Ukita², A. E. Hozain³, C. R. Flynn², M. J. Lee³, J. R. Talackine², N. L. Cardwell², J. A. Reimer³, M. Pinezich³, C. Benson², G. Vunjak-Novakovic³, S. P. Alexopoulos², M. Bacchetta²
¹co-first authors, Vanderbilt University Medical Center, Nashville, TN, ²Vanderbilt University Medical Center, Nashville, TN, ³Columbia University Medical Center, New York, NY
*Purpose: Although machine perfusion has gained momentum as an organ preservation strategy in liver transplantation, significant organ shortages and waitlist mortality persist, highlighting unmet needs…
2021 American Transplant Congress
Hepatocyte SIRT1 Suppresses Atf4-mediated Apoptosis Triggered by Cold Stress in Mouse and Human Liver Transplantation
K. Kadono¹, H. Hirao¹, H. Kojima¹, K. J. Dery¹, J. Aziz¹, X. Li², J. W. Kupiec-Weglinski¹
¹Dumont-UCLA Transplant Center, Los Angeles, CA, ²NIEHS, Durham, NC
*Purpose: The role of ER-stress or hepatocyte-specific SIRT1 signaling during donor liver cold preservation remains unknown. We employed a mouse OLT model and analyzed clinical…
2020 American Transplant Congress
Bridging the Gap: Normothermic Perfusion of Discarded Livers Significantly Alleviates the Donor Shortage
S. Raigani, R. J. De Vries, C. Carroll, Y. Chen, D. C. Chang, K. Uygun, H. Yeh
Transplant Surgery, Massachusetts General Hospital, Boston, MA
*Purpose: European studies have shown that discarded liver grafts can be successfully transplanted if they meet viability criteria during normothermic machine perfusion (NMP). The potential…
2020 American Transplant Congress
Association of Perfusion Characteristics and Post-Transplant Liver Function in Ischemia-Free Liver Transplantation
Z. Zhang, Y. Tang, Q. Zhao, Z. Guo, X. He
The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
*Purpose: It has been showed that normothermic machine perfusion (NMP), a novel preservation method, is able to assess and resuscitate liver grafts with risk factors.…
2020 American Transplant Congress
Ex Vivo Pumping Through an OPO’s Eyes
A. L. Friedman, E. Marquez
LiveOnNY, New York, NY
*Purpose: A single, large organ procurement organization (OPO) sought to document the use of extra-renal ex vivo pumping in the management of the deceased donor…
2020 American Transplant Congress
Ex-Vivo Perfusion with UW Cold Storage Solution Improves DCD Rat Liver Preservation
R. Fyffe-Freil, N. Wickramaratne, J. Khoraki, H. Kang, C. Chmielewski, R. Li, M. J. Mangino
Surgery, Virginia Commonwealth University, Richmond, VA
*Purpose: The donor pool of livers can be expanded by the successful use of donor livers recovered under DCD conditions. It is assumed that perfusion…
2020 American Transplant Congress
Normothermic Machine Perfusion of Rat Liver: Comparing Two Perfusion Methods
F. Zhou, M. Xu, O. Ahmed, G. A. Upadhya, Y. Lin, W. C. Chapman
Surgery, Washington University in St. Louis, St. Louis, MO
*Purpose: Isolated perfused rodent liver (IPRL) model has gained popularity in ex-vivo rat liver preservation and pharmacological intervention studies. Experimental conditions and system layouts vary…
2020 American Transplant Congress
DAMPs and NLRP3 Inflammasome Response in Fatty Liver Grafts Preservation in Different Preservation Solutions
R. G. Bardallo¹, R. G. Teixeira-Silva², A. Panisello-Rosello³, E. Folch-Puy³, J. Rosello Catafau⁴, T. Carbonell⁵
¹Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, Barcelona, Spain, ²Experimental Pathology, Instituto de Investigaciones Biomedicas de Barcelona, CSIC, Barcelona, Spain, ³Experimental Pathology, Instituto de Investigaciones Biomedicas de Barcelona, Barcelona, Spain, ⁴Experimental Pathology, Instituto de Investigaciones Biomédicas de Barcelona, CSIC, Barcelona, Spain, ⁵Cell Biology, Physiollogy and Immunology, Faculty of Biology, University of Barcelona, Barcelona, Spain
*Purpose: Preservation solutions (UW, HTK and IGL-1) are widely used in clinical liver transplantation. It is well known that the composition of the preservation solution…
2020 American Transplant Congress
TIM-4+ Kupffer Cells Play Homeostatic Roles in Liver Ischemia Reperfusion Injury
M. Ni¹, J. Zhang¹, R. Sosa², S. Kageyama¹, R. Busuttil¹, R. Elaine², J. Kupiec-Weglinski¹, X. Wang³, Y. Zhai¹
¹Dumont-UCLA Transplant Center, Los Angeles, CA, ²Pathology, David Geffen School of Medicine-UCLA, Los Angeles, CA, ³Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
*Purpose: Liver ischemia reperfusion injury (IRI) remains a significant clinical problem. Although the molecular mechanism of liver inflammatory response against IR has been studied extensively,…
2020 American Transplant Congress
Hypothermic Machine Perfusion Can Improve Hypoxia Tolerance in Human Discarded Liver
T. Ito, K. Kadono, H. Kojima, S. Kageyama, H. HIrao, K. Nakamura, K. J. Dery, A. E. Murray, J. Aziz, R. W. Busuttil, J. Kupiec-Weglinski, F. M. Kaldas
The Dumont-UCLA Transplant Center, UCLA, Los Angeles, CA
*Purpose: The wider use of livers from expanded criteria donors may help improve access to liver transplantation (LT). Normothermic machine perfusion in LT has been…