Articles tagged "Liver preservation"

2021 American Transplant Congress
Hypothermic Oxygenated Machine Perfusion Protects Against Cholangiocyte and Hepatocyte Injury and Mitigates Inflammation vs Static Cold Storage: Preliminary Results from a Single Center
G. Panayotova, F. Paterno, M. McCarty, G. Dikdan, S. Simonishvili, Y. Qin, L. Brown, A. Amin, K. E. Lunsford, J. V. Guarrera
Transplant Surgery, Rutgers NJMS, Newark, NJ
*Purpose: Hypothermic-oxygenated machine perfusion (HMP-O2) is a promising preservation technique with potential to mitigate ischemia/reperfusion injury and improve graft and patient outcomes. Here we present…
2021 American Transplant Congress
Role of Ferroptosis Inhibitors in Mitigating Ischemia-Reperfusion Injury in Marginal Livers Using a Novel Protect Model
M. D. Nazzal, J. Van Nispen, A. Armstrong, V. Murali, E. Song, M. Voigt, A. Samaddar, E. Madsen, C. Manithody, J. Krebs, D. Blackall, D. Carpenter, C. Varma, J. Teckman, A. Jain
Saint Louis University, Saint Louis, MO
*Purpose: The discard rate of deceased donor livers (DDL) remains high at nearly 9% and incudes livers that are steatotic, old in age, and from…
2021 American Transplant Congress
Hepatocyte SIRT1 Suppresses Atf4-mediated Apoptosis Triggered by Cold Stress in Mouse and Human Liver Transplantation
K. Kadono¹, H. Hirao¹, H. Kojima¹, K. J. Dery¹, J. Aziz¹, X. Li², J. W. Kupiec-Weglinski¹
¹Dumont-UCLA Transplant Center, Los Angeles, CA, ²NIEHS, Durham, NC
*Purpose: The role of ER-stress or hepatocyte-specific SIRT1 signaling during donor liver cold preservation remains unknown. We employed a mouse OLT model and analyzed clinical…
2021 American Transplant Congress
Cross-Circulation for Extracorporeal Liver Support in a Swine Model
W. K. Wu¹, A. Tumen¹, J. W. Stokes², R. Ukita², A. E. Hozain³, C. R. Flynn², M. J. Lee³, J. R. Talackine², N. L. Cardwell², J. A. Reimer³, M. Pinezich³, C. Benson², G. Vunjak-Novakovic³, S. P. Alexopoulos², M. Bacchetta²
¹co-first authors, Vanderbilt University Medical Center, Nashville, TN, ²Vanderbilt University Medical Center, Nashville, TN, ³Columbia University Medical Center, New York, NY
*Purpose: Although machine perfusion has gained momentum as an organ preservation strategy in liver transplantation, significant organ shortages and waitlist mortality persist, highlighting unmet needs…
2021 American Transplant Congress
Single Cell RNA Sequencing Reveals a Global Increase in Inflammatory Gene Signature Following Normothermic Ex Vivo Liver Perfusion
K. Carlson, J. Pavan-Guimaraes, B. Verhoven, J. Verhagen, F. Najmabadi, D. P. Al-Adra
Surgery, University of Wisconsin, Madison, WI
*Purpose: Normothermic ex vivo liver perfusion (NEVLP) provides an exciting system for improved organ preservation and optimized therapeutic agent delivery to a donor liver prior…
2021 American Transplant Congress
Indocyanine Green Fluorescence Imaging Allows Quantification of Ischemic Injury During Porcine Normothermic Ex Situ Perfusion
T. Goto, Y. Noguchi, S. Ganesh, L. Mazilescu, T. Reichman, N. Selzner, M. Selzner
Ajmera Transplant Program, Department of Surgery, Toronto General Hospital, Toronto, ON, Canada
*Purpose: Normothermic Ex Situ Liver Perfusion (NEsLP) is a novel preservation technique for the storage and assessment of grafts retrieved after Donation after Cardio-circulatory Death…
2021 American Transplant Congress
The Harmful Effect of Retained Peripheral Cells During Flushing and Static Cold Storage in Rat Liver Normothermic Machine Perfusion
O. Haque¹, C. A. Pendexter², J. F. Markmann², K. Uygun², H. Yeh², S. N. Tessier²
¹Surgery, Beth Israel Deaconess Medical Center, Boston, MA, ²Massachusetts General Hospital, Boston, MA
*Purpose: Retained donor blood in a liver graft can cause damage to resident cells that is exacerbated by static cold storage (SCS), leading to platelet…
2021 American Transplant Congress
Hepatic Ferroptosis in Cold Stress and Warm Ischemia-reperfusion: A Novel Therapeutic Target in Liver Transplantation
H. Kojima¹, H. Hirao¹, K. Kadono¹, T. Ito¹, K. J. Dery¹, S. Kageyama², K. Nakamura³, F. M. Kaldas¹, D. G. Farmer¹, J. W. Kupiec-Weglinski¹
¹UCLA Medical Center, Los Angeles, CA, ²Kyoto University, Kyoto, Japan, ³Nishi-Kobe Medical Center, Kyoto, Japan
*Purpose: Ferroptosis, a newly discovered programmed cell death platform, due to iron-dependent accumulation of lipid peroxides, has been reported to aggravate ischemia-reperfusion (IR) injury in…
2021 American Transplant Congress
Inhibition of Apoptosis During Ex Situ Liver Normothermic Machine Perfusion Decreases Reperfusion Injury and Improves Viability
S. Raigani¹, S. Baptista¹, A. Ohman², J. Santiago², I. Rosales¹, M. Heaney², J. Boylan², T. Coe¹, K. Uygun¹, J. Sanders², H. Yeh¹
¹Transplant Surgery, Massachusetts General Hospital, Boston, MA, ²Pediatric Endocrinology, Brown University, Providence, RI
*Purpose: Normothermic machine perfusion (NMP) is an ideal platform for delivery of novel therapeutics aimed at rehabilitation of discarded or marginal livers. We examined the…
2021 American Transplant Congress
Mitochondrial Transplant Minimizes Liver Ischemia Reperfusion Injury in Lean and Obese Mice
C. Watkins¹, G. Lamanilao¹, C. Kuscu¹, C. Kuscu¹, J. F. Pierre², J. Eason¹, A. Bajwa¹
¹Transplant Research Institute, James D. Eason Transplant Institute, Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN, ²Department of Pediatrics - Divisions of Neonatology and Pediatric Obesity, The University of Tennessee Health Science Center, Memphis, TN
*Purpose: Ischemia induces altered bioenergetics within cells with increased mitochondrial swelling and reactive oxygen species and that ultimately degrade of cellular function. Ischemia reperfusion injury…