Articles tagged "knockout"

2017 American Transplant Congress
Estrogen Receptor Alpha Mediates Female Protection from Renal Ischemia-Reperfusion Injury Through Mechanisms Extrinsic to the Kidney.
D. Aufhauser,¹ D. Murken,¹ Z. Wang,¹ G. Ge,¹ S. Concors,¹ T. Bhatti,² W. Hancock,^2,3 M. Levine.^1,4
¹Surgery, University of Pennsylvania, Philadelphia, PA; ²Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA; ³Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA; ⁴Surgery, Children's Hospital of Philadelphia, Philadelphia, PA
Introduction: Female protection from renal ischemia-reperfusion injury (IRI) is observed in both mouse and human renal transplantation. We wished to assess the role of estrogen…
2017 American Transplant Congress
TIMP-3 Plays a Key Regulatory Role in the Cleavage of E-Cadherin and Has an Essential Protective Function in Hepatic IRI.
T. Fujii, S. Duarte, R. Busuttil, A. Coito.
Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA
Liver ischemia-reperfusion injury (IRI) remains a challenging problem in clinical orthotopic liver transplantation. Tissue inhibitor of metalloproteinases-3 (TIMP-3), an extracellular matrix-associated protein, has been linked…
2017 American Transplant Congress
Dendritic Cell Expression of Rictor (mTORC2), but Not Raptor (mTORC1), Protects Against Renal Ischemia-Reperfusion Injury.
H. Dai,^1,3 N. Rogers,^1,2 A. Watson,¹ D. Fantus,¹ A. Thomson.¹
¹Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA; ²Westmead Institute for Medical Research, Sydney, Australia; ³Urologic Organ Transplantation Department, the Second Xiangya Hospital, Central South University, Changsha, China
Background: Dendritic cells (DC) are critical initiators of innate immunity in the kidney and orchestrate inflammation subsequent to ischemia-reperfusion injury (IRI). The mechanistic target of…
2017 American Transplant Congress
Macrophage Specific Deletion of RhoA Inhibits Fractalkine Signaling and Abrogates Chronic Rejection of Cardiac Allograft.
M. Kloc, Y. Liu, W. Chen, X. Li, R. Ghobrial.
Surgery, Houston Methodist Hospital and Research Institute, Houston
Objective. Chronic rejection of transplanted organs remains unresolved issue in organ transplantation. The chronic rejection is the most common cause of graft loss within 10…
2016 American Transplant Congress
Adiponectin Is Produced by Leukocytes Infiltrating Renal Transplants.
S. Goparaju, R. Fan, K. Keslar, N. Dvorina, W. Baldwin III.
Immunology, Cleveland Clinic, Cleveland, OH.
Fat is now recognized as a source of immunologically significant cytokines. Adiponectin, the most abundant adipokine, is usually classified as an anti-inflammatory cytokine because decreased…
2016 American Transplant Congress
The Fine-Tuning of CXCL8 Protects Kidney Against Ischemia-Reperfusion Injury in Mice Lacking MicroRNA-146a.
L. Amrouche,^1,2 G. Desbuissons,¹ M. Rabant,^1,2 C. Legendre,^1,2 F. Terzi,² D. Anglicheau.^1,2
¹U1151, INSERM, Paris, France, Metropolitan; ²Department of Kidney Transplantation, Necker hospital, Paris, France, Metropolitan.
Acute kidney injury leads to the development of tubular injury and interstitial inflammation that need to be controlled to avoid fibrosis development. We hypothesized that…
2016 American Transplant Congress
Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) Protects Against Loss of Hepatic Integrity After Ischemia-Reperfusion Injury.
T. Fujii, S. Duarte, R. Busuttil, A. Coito.
Surgery, The Dumont-UCLA Transplant Center, Los Angeles, CA.
Liver ischemia-reperfusion injury (IRI) remains a challenging problem in clinical orthotopic liver transplantation (OLT). Tissue inhibitor of metalloproteinases-3 (TIMP-3) is an extracellular matrix (ECM)-associated protein…
2016 American Transplant Congress
Histone Deacetylase-2 Gene Deletion in Mice Mitigates Liver Ischemia/Reperfusion Injury.
D. Murken,¹ D. Aufhauser Jr,¹ Z. Wang,² G. Ge,² W. Hancock,^2,3 M. Levine.^1,4
¹Department of Surgery, University of Pennsylvania, Philadelphia, PA; ²Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA; ³Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA; ⁴Department of Surgery, Children's Hospital of Philadelphia, Philadelphia, PA.
Introduction: Ischemia/reperfusion injury (IRI) causes significant morbidity in liver transplantation and other surgical scenarios. A better understanding of the molecular mechanisms of IRI is required…
2016 American Transplant Congress
Complement Receptor C5aR2 Deletion in Kidney Transplantation in Mice Attenuates Renal Damage.
A. Thorenz,¹ R. Chen,¹ K. Hueper,² B. Hensen,² C. Klemann,³ M. Meier,⁴ A. Klos,⁵ F. Gueler.¹
¹Nephrology, Medical School, Hannover, Germany; ²Radiology, Medical School, Hannover, Germany; ³Paediatric Surgery, Medical School, Hannover, Germany; ⁴Central Animal Facility, Medical School, Hannover, Germany; ⁵Microbiology, Medical School, Hannover, Germany.
ObjectiveThe complement receptors C5aR1 and C5aR2 serve distinct roles in immune regulation. In kidney transplantation the early post-implant ischemia reperfusion injury (IRI) causes rapid complement…
2016 American Transplant Congress
Genetic TIM-4 Deletion Promotes Immunoregulatory Tissue-Resident Macrophage Survival and Lowers the Barrier to Cardiac Allograft Tolerance.
T. Thornley,¹ P. Kyriazis,¹ K. Agarwal,¹ Z. Fang,¹ L. Ma,¹ V. Chipashvili,¹ M. Koulmanda,^1,2 T. Strom.^1,2
¹Medicine, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA; ²Surgery, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA.
We report that the CD169+TIM-4+ tissue-resident macrophage (TRM) subset is the dominant tissue-resident leukocyte in skin, heart, and pancreas from C57BL/6 mice and skin and…