Articles tagged "knockout"

2017 American Transplant Congress
Recipient iNOS Deficiency Reduces Spontaneous Kidney Allograft Acceptance by Accelerating Allograft Loss.
I. Aljabban, B. Jiang, C. Yang, D. Ndishabandi, R. White, P. Russell, J. Madsen, R. Colvin, A. Alessandrini.
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA
Nitric oxide (NO) production by inducible nitric oxide synthase (iNOS), encoded by NOS2, is a hallmark of the innate immune axis, and is typically associated…
2017 American Transplant Congress
mTORC1 and 2 Regulate Dendritic Cell (DC) Metabolism and Allostimulatory Function of Donor DCs in Skin Transplantation.
A. Watson,^1,2 H. Dai,^1,3 A. Menk,^4,5 G. Delgoffe,^4,5 A. Thomson.^1,2,3
¹Starzl Transplantation Institute, Univ. of Pittsburgh, Pittsburgh; ²Pathology, Univ. of Pittsburgh, Pittsburgh; ³Surgery, Univ. of Pittsburgh, Pittsburgh; ⁴Immunology, Univ. of Pittsburgh, Pittsburgh; ⁵Cancer Institute, Univ. of Pittsburgh, Pittsburgh
Background/Hypothesis: The mechanistic target of rapamycin (mTOR) is a kinase that functions in two complexes: rapamycin (RAPA)-sensitive mTORC1 and RAPA-insensitive mTORC2. These complexes play critical…
2017 American Transplant Congress
Estrogen Receptor Alpha Mediates Female Protection from Renal Ischemia-Reperfusion Injury Through Mechanisms Extrinsic to the Kidney.
D. Aufhauser,¹ D. Murken,¹ Z. Wang,¹ G. Ge,¹ S. Concors,¹ T. Bhatti,² W. Hancock,^2,3 M. Levine.^1,4
¹Surgery, University of Pennsylvania, Philadelphia, PA; ²Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA; ³Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA; ⁴Surgery, Children's Hospital of Philadelphia, Philadelphia, PA
Introduction: Female protection from renal ischemia-reperfusion injury (IRI) is observed in both mouse and human renal transplantation. We wished to assess the role of estrogen…
2017 American Transplant Congress
TIMP-3 Plays a Key Regulatory Role in the Cleavage of E-Cadherin and Has an Essential Protective Function in Hepatic IRI.
T. Fujii, S. Duarte, R. Busuttil, A. Coito.
Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA
Liver ischemia-reperfusion injury (IRI) remains a challenging problem in clinical orthotopic liver transplantation. Tissue inhibitor of metalloproteinases-3 (TIMP-3), an extracellular matrix-associated protein, has been linked…
2016 American Transplant Congress
TGFb in Regulatory B Cells-Dependent Tolerance.
H. Deirawan, L. Kojima, L. Washburn, J. Markmann, J. Kim.
Surgery, Massachusetts General Hospital, Boston, MA.
In transplantation, increasing evidence support that B cells can play a major regulatory role in addition to their critical functions in allo-immunity and graft rejection.…
2016 American Transplant Congress
mTORC2 Negatively Regulates Dendritic Cell (DC) Metabolism and the Allostimulatory Function of Donor DCs in Organ Transplantation.
A. Watson,^1,2 D. Raïche-Regué,¹ H. Dai,¹ K. Fabian,^3,4 G. Delgoffe,^4,5 W. Storkus,^3,4 A. Thomson.^1,2
¹Starzl Transplantation Institute, Univ. of Pittsburgh, Pittsburgh, PA; ²Pathology, Univ. of Pittsburgh, Pittsburgh, PA; ³Dermatology, Univ. of Pittsburgh, Pittsburgh, PA; ⁴Immunology, Univ. of Pittsburgh, Pittsburgh, PA; ⁵Cancer Institute, Univ. of Pittsburgh, Pittsburgh, PA.
The mechanistic target of rapamycin (mTOR) is a kinase that functions in at least two complexes: rapamycin (RAPA)-sensitive mTORC1 and RAPA-insensitive mTORC2. These complexes play…
2016 American Transplant Congress
Role of NRP1 in CD4+ T Cell Allo-Activation.
E. Lee, H. Kim, G. Demirci.
Department of Surgery, Boston Children's Hospital, Boston, MA.
Introduction: Neuropilin-1 (NRP1) is a transmembrane glycoprotein that functions as a co-receptor for class III/IV semaphorins, VEGF, and several other growth factors. NRP1 has been…
2016 American Transplant Congress
Gsk3β Regulates Macrophage Pro-Inflammatory Immune Activation via AMPK-Mediated Induction of Small Heterodimer Partner (SHP).
H. Zhou,^1,2 J. Zhu,^1,3 S. Yue,¹ R. Busuttil,¹ J. Kupiec-Weglinski,¹ X. Wang,² Y. Zhai.¹
¹Surgery, David Geffen School of Medicine-University of California-Los Angeles, LA, CA; ²Liver Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China; ³Liver Surgery, Renji Hospital, Shanghai, China.
Background & AimsAlthough glycogen synthase kinase 3 β (Gsk3β) has been found to play key roles in ischemia and reperfusion injuries (IRI) of different organs,…
2016 American Transplant Congress
Macrophage Specific Deletion of RhoA Impedes Macrophage Functions and Abrogates Chronic Rejection of Cardiac Allograft.
Y. Liu, W. Chen, X. Li, R. Ghobrial, M. Kloc.
Surgery, The Houston Methodist, Houston, TX.
Objective. Chronic rejection of transplanted organs remains the main obstacle in the long-term success of organ transplantation. Chronic rejection occurs in 40-45% of recipients at…
2016 American Transplant Congress
HO-1/SIRT1/p53 Axis Regulates Macrophage Activation and Attenuates Liver Ischemia-Reperfusion Injury in Mice.
K. Nakamura,¹ M. Zhang,² B. Ke,¹ S. Kageyama,¹ R. Busuttil,¹ J. Araujo,² J. Kupiec-Weglinski.¹
¹Surgery, Liver Transplant, University of California-Los Angeles, Los Angeles, CA; ²Medicine, Division of Cardiology, University of California-Los Angeles, Los Angeles, CA.
Background/Aim: The mechanism by which macrophage heme oxygenase-1 (HO-1)-Sirtuin1 (SIRT1) signaling promotes resistance against inflammation response in liver ischemia-reperfusion injury (IRI) remains elusive. As tumor…