ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Complement Receptor C5aR2 Deletion in Kidney Transplantation in Mice Attenuates Renal Damage.

A. Thorenz,1 R. Chen,1 K. Hueper,2 B. Hensen,2 C. Klemann,3 M. Meier,4 A. Klos,5 F. Gueler.1

1Nephrology, Medical School, Hannover, Germany
2Radiology, Medical School, Hannover, Germany
3Paediatric Surgery, Medical School, Hannover, Germany
4Central Animal Facility, Medical School, Hannover, Germany
5Microbiology, Medical School, Hannover, Germany.

Meeting: 2016 American Transplant Congress

Abstract number: D65

Keywords: Kidney transplantation, knockout, Mice, Renal ischemia, T cell activation

Session Information

Date: Tuesday, June 14, 2016

Session Name: Poster Session D: Chimerism/Stem Cells, Cellular/Islet Transplantation, Innate Immunity, Chronic Rejection

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Halls C&D

Related Abstracts
  • Distinct Role of Complement Receptors C5L2 and C5aR in Acute Kidney Injury and Renal Fibrosis
  • HO-1/SIRT1/p53 Axis Regulates Macrophage Activation and Attenuates Liver Ischemia-Reperfusion Injury in Mice.

Objective

The complement receptors C5aR1 and C5aR2 serve distinct roles in immune regulation. In kidney transplantation the early post-implant ischemia reperfusion injury (IRI) causes rapid complement activation. So far, there is little known about the distinct role of C5aR1 and C5aR1. In this study C5aR1 and C5aR2 deficient mice were tested in two different models: renal ischemia reperfusion injury (IRI) and in isogenic cross over kidney transplantation (ktx).

Methods

IRI was inducedby unilateral clipping of the right renal pedicle for 45 min in C5aR1, C5aR2 deficient and wild type mice (WT). Functional magnetic resonance imaging (MRI) to analyze renal perfusion was performed at different time points (d1, d7, week3) and glomerular filtration rate (GFR) were assessed by inulin clearance. Renal morphology, inflammation and renal fibrosis were investigated by immunohistochemistry. In a second model isogenic ktx with prolonged cold ischemia time of 90 min was performed. C5aR1, C5aR2 or WT mice served as donors or recipients.

Results

C5aR2 deficiency resulted in attenuated inflammation and renal fibrosis 3 weeks are IRI. Kidney regeneration was enhanced in C5aR2 deficient mice which showed significantly improved renal perfusion. In the ktx model C5R2 deficiency of the recipient showed the best renal morphology with attenuated renal injury. By FACS analysis differential activation in gd-Tcells in C5aR2 deficient mice was observed and may have contributed to enhanced regeneration.

Conclusion

C5aR2 deficiency attenuates renal damage in AKI and ktx via modulation of T-cell signalling.

CITATION INFORMATION: Thorenz A, Chen R, Hueper K, Hensen B, Klemann C, Meier M, Klos A, Gueler F. Complement Receptor C5aR2 Deletion in Kidney Transplantation in Mice Attenuates Renal Damage. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Thorenz A, Chen R, Hueper K, Hensen B, Klemann C, Meier M, Klos A, Gueler F. Complement Receptor C5aR2 Deletion in Kidney Transplantation in Mice Attenuates Renal Damage. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/complement-receptor-c5ar2-deletion-in-kidney-transplantation-in-mice-attenuates-renal-damage/. Accessed February 27, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
      • Penis Transplantation: First U.S. Experience.
      • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
      • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
      • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
      • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

      Visit Our Partner Sites

      American Transplant Congress (ATC)

      Visit the official site for the American Transplant Congress »

      American Journal of Transplantation

      The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

      American Society of Transplantation (AST)

      An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

      American Society of Transplant Surgeons (ASTS)

      The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

      Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

      Privacy Policy

      loading Cancel
      Post was not sent - check your email addresses!
      Email check failed, please try again
      Sorry, your blog cannot share posts by email.
      This site uses cookies: Find out more.