Articles tagged "knockout"

2016 American Transplant Congress
Abrogating Donor-Derived Myd88 Signaling Impairs the Recruitment of Recipient Neutrophils and CD8 T Cells into Lung Allografts in Mice.
Z. Zheng,¹ J.-J. Wang,¹ X. Yeap,¹ X. Kang,¹ Y. Xie,¹ X. Luo,² Z. Zhang.¹
¹Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL; ²Division of Nephrology and Hypertension, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL.
Objectives: Transplant ischemia reperfusion injury (IRI) mediated primary graft dysfunction (PGD) remains the predominant cause of short- and long-term graft loss in lung transplantation. This…
2016 American Transplant Congress
Absence of Receptors for C3a or C5a Prevent Alloreactive CD8 T Effector Cell (Teff) Expansion but Differentially Impact Memory (Tmem) Persistence Posttransplantation.
D. Mathern, P. Heeger.
Translational Transplant Research Center, Dept of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY.
As Tmem are known barriers to transplant survival and tolerance in animals and human transplant recipients, understanding factors that drive the formation and survival of…
2016 American Transplant Congress
Genetic TIM-4 Deletion Promotes Immunoregulatory Tissue-Resident Macrophage Survival and Lowers the Barrier to Cardiac Allograft Tolerance.
T. Thornley,¹ P. Kyriazis,¹ K. Agarwal,¹ Z. Fang,¹ L. Ma,¹ V. Chipashvili,¹ M. Koulmanda,^1,2 T. Strom.^1,2
¹Medicine, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA; ²Surgery, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA.
We report that the CD169+TIM-4+ tissue-resident macrophage (TRM) subset is the dominant tissue-resident leukocyte in skin, heart, and pancreas from C57BL/6 mice and skin and…
2016 American Transplant Congress
Complement Receptor C5aR2 Deletion in Kidney Transplantation in Mice Attenuates Renal Damage.
A. Thorenz,¹ R. Chen,¹ K. Hueper,² B. Hensen,² C. Klemann,³ M. Meier,⁴ A. Klos,⁵ F. Gueler.¹
¹Nephrology, Medical School, Hannover, Germany; ²Radiology, Medical School, Hannover, Germany; ³Paediatric Surgery, Medical School, Hannover, Germany; ⁴Central Animal Facility, Medical School, Hannover, Germany; ⁵Microbiology, Medical School, Hannover, Germany.
ObjectiveThe complement receptors C5aR1 and C5aR2 serve distinct roles in immune regulation. In kidney transplantation the early post-implant ischemia reperfusion injury (IRI) causes rapid complement…
2016 American Transplant Congress
Histone Deacetylase-2 Gene Deletion in Mice Mitigates Liver Ischemia/Reperfusion Injury.
D. Murken,¹ D. Aufhauser Jr,¹ Z. Wang,² G. Ge,² W. Hancock,^2,3 M. Levine.^1,4
¹Department of Surgery, University of Pennsylvania, Philadelphia, PA; ²Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA; ³Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA; ⁴Department of Surgery, Children's Hospital of Philadelphia, Philadelphia, PA.
Introduction: Ischemia/reperfusion injury (IRI) causes significant morbidity in liver transplantation and other surgical scenarios. A better understanding of the molecular mechanisms of IRI is required…
2016 American Transplant Congress
Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) Protects Against Loss of Hepatic Integrity After Ischemia-Reperfusion Injury.
T. Fujii, S. Duarte, R. Busuttil, A. Coito.
Surgery, The Dumont-UCLA Transplant Center, Los Angeles, CA.
Liver ischemia-reperfusion injury (IRI) remains a challenging problem in clinical orthotopic liver transplantation (OLT). Tissue inhibitor of metalloproteinases-3 (TIMP-3) is an extracellular matrix (ECM)-associated protein…
2016 American Transplant Congress
The Fine-Tuning of CXCL8 Protects Kidney Against Ischemia-Reperfusion Injury in Mice Lacking MicroRNA-146a.
L. Amrouche,^1,2 G. Desbuissons,¹ M. Rabant,^1,2 C. Legendre,^1,2 F. Terzi,² D. Anglicheau.^1,2
¹U1151, INSERM, Paris, France, Metropolitan; ²Department of Kidney Transplantation, Necker hospital, Paris, France, Metropolitan.
Acute kidney injury leads to the development of tubular injury and interstitial inflammation that need to be controlled to avoid fibrosis development. We hypothesized that…
2016 American Transplant Congress
Adiponectin Is Produced by Leukocytes Infiltrating Renal Transplants.
S. Goparaju, R. Fan, K. Keslar, N. Dvorina, W. Baldwin III.
Immunology, Cleveland Clinic, Cleveland, OH.
Fat is now recognized as a source of immunologically significant cytokines. Adiponectin, the most abundant adipokine, is usually classified as an anti-inflammatory cytokine because decreased…
2016 American Transplant Congress
HO-1/SIRT1/p53 Axis Regulates Macrophage Activation and Attenuates Liver Ischemia-Reperfusion Injury in Mice.
K. Nakamura,¹ M. Zhang,² B. Ke,¹ S. Kageyama,¹ R. Busuttil,¹ J. Araujo,² J. Kupiec-Weglinski.¹
¹Surgery, Liver Transplant, University of California-Los Angeles, Los Angeles, CA; ²Medicine, Division of Cardiology, University of California-Los Angeles, Los Angeles, CA.
Background/Aim: The mechanism by which macrophage heme oxygenase-1 (HO-1)-Sirtuin1 (SIRT1) signaling promotes resistance against inflammation response in liver ischemia-reperfusion injury (IRI) remains elusive. As tumor…
2016 American Transplant Congress
Macrophage Specific Deletion of RhoA Impedes Macrophage Functions and Abrogates Chronic Rejection of Cardiac Allograft.
Y. Liu, W. Chen, X. Li, R. Ghobrial, M. Kloc.
Surgery, The Houston Methodist, Houston, TX.
Objective. Chronic rejection of transplanted organs remains the main obstacle in the long-term success of organ transplantation. Chronic rejection occurs in 40-45% of recipients at…