Articles tagged "Image analysis"

2016 American Transplant Congress
The In Vivo Non-Invasive Long-Term Functional Assessment of Neuronal Regeneration in Vascularized Composite Allotransplantation.
C.-J. Wen,^1,2 C.-H. Lin,¹ S.-L. Peng,^3,4 F.-N. Wang,³ L.-Y. Huang,³ H.-Y. Cheng,¹ H.-H. Hsu,¹ X.-T. Huang,¹ C.-H. Lin,¹ F.-C. Wei.¹
¹Center for Vascularized Composite Allotransplantation, Chang Gung Medical Foundation, Taoyuan, Taiwan; ²Department of Medicine, Chang Gung University, Taoyuan, Taiwan; ³Department of Biomedical Engineering and Environmental Sciences, Tsing Hua University, Hsinchu, Taiwan; ⁴Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan.
The neuronal regeneration is a key issue of vascularized composite allotransplantation (VCA).As for the generally used functional observations, behavior testing or invasive functional inspection (e.g.…
2016 American Transplant Congress
A Quantitative CD45 Immunohistochemical Assay Allows Precise and Reproducible Measurement of Inflammation in Renal Transplant Biopsies.
V. Walavalkar,¹ S. Bowman,¹ L. Laszik,¹ D. Ikle,² S. Chandran,¹ F. Vincenti,¹ Z. Laszik.¹
¹UCSF, San Francisco, CA; ²Rho, Chapel Hill, NC.
Purpose: Total inflammation (TI) is an important variable for the pathologic work-up of transplant kidney biopsies (TxBx) in the Banff schema. However, current standard pathologic…
2016 American Transplant Congress
A Novel Quantitative CD45 Immunofluorescence Assay Can Measure Inflammatory Load in Renal Transplant Biopsies with Excellent Reproducibility and Repeatability.
Z. Laszik,¹ D. Dobi,¹ L. Laszik,¹ D. Ikle,² S. Chandran,¹ F. Vincenti.¹
¹UCSF, San Francisco, CA; ²Rho, Chapel Hill, NC.
Purpose: The interobserver reproducibility of the current standard semiquantitative (Banff) grading of inflammation in renal transplant biopsies (TxBx) is suboptimal. We have recently developed a…
2016 American Transplant Congress
A Quantitative CD45 Assay May Improve Diagnostic Accuracy of the Diagnosis of Acute Cellular Rejection in Transplant Kidney Biopsies.
L. Laszik, S. Bowman, V. Walavalkar, F. Vincenti, Z. Laszik.
UCSF, San Francisco.
Purpose: We have previously shown that the total inflammatory load in transplant kidney biopsies (TxBx) can be determined with a high degree of accuracy using…
2016 American Transplant Congress
Identification of Biomarkers for Progressive Fibrosis in Renal Transplant Patients Using Novel Chemical Imaging Approaches.
V. Varma,¹ A. Kajdacsy-Balla,¹ S. Akkina,² S. Setty,¹ M. Walsh.¹
¹Pathology, University of Illinois at Chicago, Chicago, IL; ²Nephrology, University of Illinois at Chicago, Chicago, IL.
BackgroundThe main treatment for end-stage renal disease is kidney transplantation, however close monitoring of post-transplant biopsies is required to identify subclinical complications. In this study,…
2016 American Transplant Congress
Imaging Cell Biology In Vivo with Intravital-Microscopy.
T. Ueno,¹ M. Yeung,¹ N. Igarashi,³ T. Yokoyama,² Y. Kihara,² O. Konno,² Y. Nakamura,² H. Iwamoto,² T. Ikeda,³ M. McGrath,¹ M. Sayegh,¹ A. Chandraker.¹
¹Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Boston, MA; ²Transplant Surgery, Tokyo Medical Univ., Hachioji, Tokyo, Japan; ³Intensive Care Medicine, Tokyo Medical Univ., Hachioji, Tokyo, Japan.
Background: Despite current immunosuppressive therapy, many recipients endure multiple episodes of acute rejection. The interplay between dendritic cells (DCs) and T cells plays a central…
2016 American Transplant Congress
Digital Kidney Biopsy Evaluation: Quantification of Macrophage Prevalence in a Cross Sectional Study.
J. Schmitz,¹ A. Khalifa,¹ H. Haller,² H. Kreipe,¹ F. Feuerhake,¹ J. Bräsen.¹
¹Institute of Pathology, Hannover Medical School, Hannover, Germany; ²Department of Nephrology and Hypertension, Hannover Medical School, Hannover, Germany.
Standardized markers based on quantitative and qualitative evaluation of immune cell mass and localization in kidney biopsies may help in diagnosis and prognosis in transplantation…
2016 American Transplant Congress
Improving the Efficiency, Safety and Cost of Evaluating Living Donor Liver Candidates Using Single Sequence MRI.
M. Schilsky,^1,2 K. Cartiera,¹ S. Boga,¹ S. Schilsky,³ S. Emre,¹ D. Mulligan,¹ J. Weinreb,¹ G. Israel.¹
¹Yale New Haven Transplantation Center, Transplantation and Immunology, Surgery, Yale University, New Haven, CT; ²Section of Digestive Diseases, Medicine, Yale University, New Haven, CT; ³Food and Drug Administration, Silver Springs, MD.
Background: Current evaluation of donor candidates for adult-to-adult living donor liver transplant (LDLT) includes MRI and CT without and with contrast for defining liver anatomy.…
2015 American Transplant Congress
Inverse Monocytic Subset Profile in Blood and Tissue During Heart Transplant Rejection
T. van den Bosch,¹ M. Kraaij,¹ K. Caliskan,² A. Constantinescu,² O. Manintveld,² P. Leenen,³ C. Baan,¹ M. van Groningen,⁴ A. Rowshani.¹
¹Department of Internal Medicine, Section Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, Netherlands; ²Department of Cardiology, Erasmus Medical Center, Rotterdam, Netherlands; ³Department of Immunology, Erasmus Medical Center, Rotterdam, Netherlands; ⁴Department of Pathology, Erasmus Medical Center, Rotterdam, Netherlands.
Introduction: Increased monocytic infiltration of heart transplant tissue is documented during acute rejection. It is still unclear how changes in circulating monocyte pool relate to…
2015 American Transplant Congress
Evaluating the Benefits of Everolimus on Cardiac Allograft Vasculopathy: Further Evidence from Results of Imputations for Missing IVUS Data from A2310 Study
P. Lopez,¹ G. Dong,² Z. Wang.²
¹Novartis Pharma AG, Basel, Switzerland; ²Novartis Pharmaceuticals, East Hanover, NJ; ³Novartis Pharmaceuticals, East Hanover, NJ.
Purpose: A2310 IVUS study showed that everolimus (EVR) provides cardiac allograft vasculopathy (CAV) benefit compared to mycophenolate mofetil (MMF) by assessing the mean change in…