ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

A Novel Quantitative CD45 Immunofluorescence Assay Can Measure Inflammatory Load in Renal Transplant Biopsies with Excellent Reproducibility and Repeatability.

Z. Laszik,1 D. Dobi,1 L. Laszik,1 D. Ikle,2 S. Chandran,1 F. Vincenti.1

1UCSF, San Francisco, CA
2Rho, Chapel Hill, NC.

Meeting: 2016 American Transplant Congress

Abstract number: A155

Keywords: Biopsy, Graft-infiltrating lymphocytes, Image analysis, Kidney

Session Information

Date: Saturday, June 11, 2016

Session Name: Poster Session A: Kidney: Acute Cellular Rejection

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Halls C&D

Related Abstracts
  • A Quantitative CD45 Immunohistochemical Assay Allows Precise and Reproducible Measurement of Inflammation in Renal Transplant Biopsies.
  • A Quantitative CD45 Assay May Improve Diagnostic Accuracy of the Diagnosis of Acute Cellular Rejection in Transplant Kidney Biopsies.

Purpose: The interobserver reproducibility of the current standard semiquantitative (Banff) grading of inflammation in renal transplant biopsies (TxBx) is suboptimal. We have recently developed a highly reproducible quantitative CD45 (leukocyte common antigen) immunoperoxidase assay to measure total inflammation (TI) in TxBx. The goal of the current study is to validate a quantitative CD45 assay on an immunofluorescent (IF) platform with the future aim of developing multiplexing technologies for tissue-based phenotypical and genotypic analysis.

Methods: Thirty two serially cut 2 [mu]m-thick consecutive sections of formalin-fixed paraffin-embedded (FFPE) tissues from 2 TxBx were stained for CD45 on a Leica Bond RX platform using a standardized indirect IF protocol on 2 consecutive days (sections 1-2, 5-6, etc. stained on day 1, and sections 3-4, 7-8, etc. stained on day 2). Whole slide scans of the IF sections generated on a Zeiss Axio Imager Z2 scope were analyzed using the ImageJ software. The CD45 positive cells were quantitated in each image and linear regression analysis was used to assess the reproducibility and the repeatability of the assay performed on the same day and on separate days on consecutive sections.

Results: The quality of 3 sections from 3 different pairs was suboptimal and therefore these 3 pairs were excluded from the analysis. Linear regression analysis showed the following parameter estimates and their 95% confidence intervals for the two analyses (i: same day; ii: separate day): (i) slope = 0.9761 (0.7474, 1.205), y intercept = 0.0565 (-0.7164, 0.8293), and R2 = 0.8892; (ii) slope = 0.9343 (0.5226 to 1.346), y-intercept = 0.1845 (-1.261 to 1.630), and R2 = 0.7739.

Conclusions: The results demonstrate excellent reproducibility and repeatability for the standardized quantitative CD45 IF assay on FFPE TxBx. The test will allow precise quantitation of inflammation in TxBx to monitor therapeutic responses in follow-up biopsies in Clinical Trials in Organ Transplantation and in routine biopsy setting. The assay lays the foundation for future development and implementation of tissue-based IF multiplexing tests in transplantation.

CITATION INFORMATION: Laszik Z, Dobi D, Laszik L, Ikle D, Chandran S, Vincenti F. A Novel Quantitative CD45 Immunofluorescence Assay Can Measure Inflammatory Load in Renal Transplant Biopsies with Excellent Reproducibility and Repeatability. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Laszik Z, Dobi D, Laszik L, Ikle D, Chandran S, Vincenti F. A Novel Quantitative CD45 Immunofluorescence Assay Can Measure Inflammatory Load in Renal Transplant Biopsies with Excellent Reproducibility and Repeatability. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/a-novel-quantitative-cd45-immunofluorescence-assay-can-measure-inflammatory-load-in-renal-transplant-biopsies-with-excellent-reproducibility-and-repeatability/. Accessed January 19, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.