ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

A Quantitative CD45 Immunohistochemical Assay Allows Precise and Reproducible Measurement of Inflammation in Renal Transplant Biopsies.

V. Walavalkar,1 S. Bowman,1 L. Laszik,1 D. Ikle,2 S. Chandran,1 F. Vincenti,1 Z. Laszik.1

1UCSF, San Francisco, CA
2Rho, Chapel Hill, NC.

Meeting: 2016 American Transplant Congress

Abstract number: A153

Keywords: Biopsy, Graft-infiltrating lymphocytes, Image analysis, Kidney

Session Information

Date: Saturday, June 11, 2016

Session Name: Poster Session A: Kidney: Acute Cellular Rejection

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Halls C&D

Related Abstracts
  • A Novel Quantitative CD45 Immunofluorescence Assay Can Measure Inflammatory Load in Renal Transplant Biopsies with Excellent Reproducibility and Repeatability.
  • A Quantitative CD45 Assay May Improve Diagnostic Accuracy of the Diagnosis of Acute Cellular Rejection in Transplant Kidney Biopsies.

Purpose: Total inflammation (TI) is an important variable for the pathologic work-up of transplant kidney biopsies (TxBx) in the Banff schema. However, current standard pathologic assessment of the inflammation is imprecise and the interobserver reproducibility of the Banff grading is suboptimal. The aim of our study was to design and validate a quantitative CD45 (leukocyte common antigen) immunohistochemical (IHC) assay for precise, objective and reproducible measurement of TI in TxBx.

Methods: Transplant kidney biopsies with normal morphology (NL) (n=15), borderline change (BL) (n=17), and acute cellular rejection (ACR) (n=23) were used for the study. From each case, serial 3 [mu]m-thick sections cut in a routine histology lab were stained for CD45 on a Leica Bond RX platform using standardized IHC protocol on 2 consecutive days. Whole slide images generated on Aperio AT2 scanner were analyzed using Definiens Tissue Studio 4.1 software to measure the total number of cells and the total number of CD45 positive cells in the cortical portion of the biopsies. Linear regression analysis was used to assess the repeatability of the assay with the IHC stains performed on separate days on paired consecutive sections.

Results: Fifteen cases in which the total cell count difference in the paired sections exceeded 10 percent indicative of significant cell content mismatch were excluded from the analysis. The remaining 40 cases [NL (n=10); BL (n=16) and ACR (n=14)] showed an average number of 13234 ± SE 2072 vs. 12924 ± SE 2032 CD45 positive cells in the paired sections (p= 0.915). Linear regression analysis of day 1 vs day 2 values showed a slope of 0.9248 (95% CI) y-intercept (-776.9 to 927.7), and R2= 0.9796 in the paired sections.

Conclusions: The results demonstrate excellent repeatability for the standardized quantitative CD45 IHC assay on TxBx. The test will allow objective, precise, and reproducible quantitation of inflammation in TxBx to monitor therapeutic responses in follow-up biopsies in Clinical Trials in Organ Transplantation and also in routine biopsy setting. The test can be readily implemented in routine pathology laboratories.

CITATION INFORMATION: Walavalkar V, Bowman S, Laszik L, Ikle D, Chandran S, Vincenti F, Laszik Z. A Quantitative CD45 Immunohistochemical Assay Allows Precise and Reproducible Measurement of Inflammation in Renal Transplant Biopsies. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Walavalkar V, Bowman S, Laszik L, Ikle D, Chandran S, Vincenti F, Laszik Z. A Quantitative CD45 Immunohistochemical Assay Allows Precise and Reproducible Measurement of Inflammation in Renal Transplant Biopsies. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/a-quantitative-cd45-immunohistochemical-assay-allows-precise-and-reproducible-measurement-of-inflammation-in-renal-transplant-biopsies/. Accessed January 19, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.