Articles tagged "Histocompatibility"

2022 American Transplant Congress
ABO Hemagglutination Titres Do Not Accurately Reflect ABO Antibody Levels
A. Halpin¹, T. Ellis², J. Pearcey², B. Motyka², T. L. Lowary³, C. Cairo⁴, S. Maier², L. J. West⁵
¹Pediatrics/Dept of Laboratory Medicine, University of Alberta/Alberta Precision Laboratories, Edmonton, AB, Canada, ²Pediatrics, University of Alberta, Edmonton, AB, Canada, ³Academia Sinica, Institute of Biological Chemistry, Taipei, Taiwan, ⁴Chemistry, University of Alberta, Edmonton, AB, Canada, ⁵Pediatrics, University of Alberta, Canadian Donation and Transplant Research Program, Edmonton, AB, Canada
*Purpose: ABO antibody (ABO-Ab) quantification is essential for risk assessment for ABO-incompatible (ABOi) transplantation, but the hemagglutination assay (HA) for ABO-Ab titres is known to…
2022 American Transplant Congress
Beyond CPRA: Identifying Sensitized Kidney Candidates with Markedly Low Access to Transplant
K. Bradbrook¹, A. Maldonado², K. Sjöholm², C. Kjellman², J. Lee³, D. Stewart¹
¹UNOS, Richmond, VA, ²Hansa Biopharma AB, Lund, Sweden, ³Immucor, Norcross, GA
*Purpose: The Kidney Allocation System (KAS) improved the rates of transplantation in highly sensitized (HS) patients. However, a subset of HS patients remain at a…
2022 American Transplant Congress
Production of Exosomal IL35 from Alloreactive Treg Cells: A Basis for Potent Immunotherapy and Tolerance in Human Kidney Transplantation
W. J. Burlingham¹, E. Jankowska-Gan², J. Fechner¹, C. Little¹, S. Tripathi³, B. Schreiber³, A. Chandraker³, D. Foley⁴
¹Surgery-Transplant Division, University of Wisconsin, Madison, WI, ²Surgery-Transplant Division, University of Wisconsin-Madison, Madison, WI, ³Surgery-Transplant Division, Brigham & Women's Hospital, Boston, MA, ⁴University of Wisconsin, Madison, WI
*Purpose: Treg cells make a variety of immunosuppressive products—cytokines such as IL10, TGFβ, and IL35, and enzymes such as the CD39/CD73 pair that convert extracellular…
2022 American Transplant Congress
HLA-Specific IgD/CD27 Double Negative B Cell Frequency Assessed by a Novel Multiplex Bead-Based Assay Predicts Rejection in Kidney Transplant Recipients
Z. Ilyas¹, A. Akl², D. Pinelli¹, L. Gallon³, A. Tambur¹, M. Ansari⁴
¹Northwestern University, Chicago, IL, ²Dr. Soliman Fakeeh Hospital, Jeddah, Saudi Arabia, ³Northwestern Univ NMFF, Chicago, IL, ⁴Medicine, Northwestern University, Chicago, IL
*Purpose: Recipients with sensitization to HLA defined by a high percentage of Panel Reactive Antibodies (PRA) or Donor-specific anti-HLA antibodies (DSA) are at higher immunologic…
2022 American Transplant Congress
Stem Cell Donor-Derived CPRA Better Reflects Sensitization Than Current CPRA
K. Lindblad¹, C. Murphey², P. Lalli³, J. Lunz⁴, L. Gragert⁵
¹UNOS, Richmnd, VA, ²Southwest Immunodiagnostics, Inc., San Antonio, TX, ³Carolinas Medical Center, Charlotte, NC, ⁴LifeLink Foundation Transplant Immunology Lab, Tampa, FL, ⁵Tulane University School of Medicine, New Orleans, LA
*Purpose: The OPTN Histocompatibility Committee proposes replacing the current calculated panel reactive antibody (CPRA), based on HLA haplotype frequencies from ~14,000 deceased kidney donors, with…
2021 American Transplant Congress
Blood Type A2/a2b to B Renal Transplantation: A Single Center Retrospective Cohort Study
V. S. Tatapudi, N. Alnazari, R. Chand, N. M. Ali, B. E. Lonze, R. A. Montgomery
NYU Langone Transplant Institute, NYU Langone Health, New York, NY
*Purpose: Blood type B candidates on the deceased donor kidney waitlist have a lower transplantation rate and longer wait time than candidates of other blood…
2021 American Transplant Congress
Use of a Novel Bead-based Assay to Measure the Impact of Imlifidase on ABO IgG Antibodies
A. Halpin¹, B. Motyka², T. Ellis², J. Pearcey², T. L. Lowary³, A. Runström⁴, L. Winstedt⁴, R. Bockermann⁴, S. Järnum⁴, A. Robertson⁴, L. J. West⁵
¹Department of Pediatrics and Laboratory Medicine and Pathology, University of Alberta and Alberta Precision Laboratories, Edmonton, AB, Canada, ²Department of Pediatrics, University of Alberta, Edmonton, AB, Canada, ³Department of Chemistry, University of Alberta, Edmonton, AB, Canada, ⁴Hansa Biopharma, Lund, Sweden, ⁵Department of Pediatrics and Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
*Purpose: The accurate assessment and measurement of ABO antibodies (ABO-Ab) is a critical component of ABO-incompatible (ABOi) transplantation. ABO-Ab comprise both IgG and IgM, which…
2021 American Transplant Congress
Are All DSA’s the Same? Are We Ready for Precision Medicine in Pediatric LT?
S. E. Shin¹, M. Lee¹, N. Yazigi², K. Khan², S. Kaufman², J. Ahn³, O. Timofeeva⁴, U. Ekong²
¹Georgetown University School of Medicine, Washington, DC, ²Medstar Georgetown Transplant Institute, Medstar Georgetown University Hospital, Washington, DC, ³Biostatistics, Bioinformatics, & Biomathematics, Georgetown University, Washington, DC, ⁴Histocompatibility Laboratory, Dept. of Pathology & Laboratory Medicine, Georgetown University, Washington, DC
*Purpose: Donor-specific antibodies (DSA) play an uncertain role in rejection after liver transplantation (LT). We hypothesized that evaluation of epitope differences in HLA-DR/DQ mismatching can…
2021 American Transplant Congress
Disparities in HLA Representation by Single Antigen Reagents in Different Ethnic Populations
J. C. Quon¹, J. Menteer², N. Fotiadis², R. M. Lestz², L. A. Baxter-Lowe²
¹Keck School of Medicine of USC, Los Angeles, CA, ²Children's Hospital Los Angeles, Los Angeles, CA
*Purpose: It is not possible to test for antibodies against the antigens encoded by every HLA allele due to cost and throughput constraints. When alleles…
2021 American Transplant Congress
HLA Mismatching and Subclinical Inflammation: An Association to be Considered in Kidney Transplant Patients with Low Immunological Risk
T. Vazquez-Sanchez¹, J. Alonso-Titos¹, P. Ruiz-Esteban¹, A. Caballero², M. Leon³, V. Lopez¹, E. Sola¹, D. Hernández¹
¹Nephrology, Instituto de Investigación Biomédica de Málaga (IBIMA). Hospital Universitario Regional de Málaga and University of Málaga. REDinREN (RD16/0009/0006). Málaga. (Spain), Málaga, Spain, ²Immunology, Instituto de Investigación Biomédica de Málaga (IBIMA). Hospital Universitario Regional de Málaga and University of Málaga. REDinREN (RD16/0009/0006). Málaga. (Spain), Málaga, Spain, ³Pathology, Instituto de Investigación Biomédica de Málaga (IBIMA). Hospital Universitario Regional de Málaga and University of Málaga. REDinREN (RD16/0009/0006). Málaga. (Spain), Málaga, Spain
*Purpose: Subclinical inflammation (SCI) (