Articles tagged "Histocompatibility"

2022 American Transplant Congress
Complete and High-Resolution HLA Typing Improves the Accuracy of Post-Transplant Assessment of De Novo Anti-Donor Immune-Mediated Events
M. Meneghini¹, A. Perona², E. Crespo³, F. J. Bemelman⁴, P. M. Reinke⁵, O. Viklicky⁶, M. Giral⁷, O. Bestard¹
¹Vall d'Hebron University Hospital, Barcelona, Spain, ²Universitat de Barcelona, Barcelona, Spain, ³Nephrology and Transplantation Laboratory, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain, ⁴Amsterdam University Medical Centers, Amsterdam, Netherlands, ⁵Charité Universitätsmedizin, Berlin, Germany, ⁶Institute for Clinical and Experimental Medicine (IKEM), Prague, Czech Republic, ⁷Nantes Université, Inserm, CHU Nantes, Nantes, France
*Purpose: High-resolution (HR) HLA typing using NGS technology has shown to improve the accuracy of assessing donor-recipient compatibility and characterization of pre-transplant anti HLA antibodies…
2022 American Transplant Congress
High Resolution Typing Can Improve Accuracy and Sensitivity of the Virtual Crossmatch for Sensitized Patients – A Single Center’s Experience
C. MarElia-Bennett¹, V. Rohan², K. Twombley³, O. Moussa¹
¹Pathology & Laboratory Medicine, Medical University of South Carolina, Charleston, SC, ²Surgery, Medical University of South Carolina, Charleston, SC, ³Medical University of South Carolina, Charleston, SC
*Purpose: The introduction of solid phase assays has significantly enhanced our ability to execute virtual crossmatches (VXMs). However, this method is not without its shortcomings.…
2022 American Transplant Congress
ABO Hemagglutination Titres Do Not Accurately Reflect ABO Antibody Levels
A. Halpin¹, T. Ellis², J. Pearcey², B. Motyka², T. L. Lowary³, C. Cairo⁴, S. Maier², L. J. West⁵
¹Pediatrics/Dept of Laboratory Medicine, University of Alberta/Alberta Precision Laboratories, Edmonton, AB, Canada, ²Pediatrics, University of Alberta, Edmonton, AB, Canada, ³Academia Sinica, Institute of Biological Chemistry, Taipei, Taiwan, ⁴Chemistry, University of Alberta, Edmonton, AB, Canada, ⁵Pediatrics, University of Alberta, Canadian Donation and Transplant Research Program, Edmonton, AB, Canada
*Purpose: ABO antibody (ABO-Ab) quantification is essential for risk assessment for ABO-incompatible (ABOi) transplantation, but the hemagglutination assay (HA) for ABO-Ab titres is known to…
2022 American Transplant Congress
Beyond CPRA: Identifying Sensitized Kidney Candidates with Markedly Low Access to Transplant
K. Bradbrook¹, A. Maldonado², K. Sjöholm², C. Kjellman², J. Lee³, D. Stewart¹
¹UNOS, Richmond, VA, ²Hansa Biopharma AB, Lund, Sweden, ³Immucor, Norcross, GA
*Purpose: The Kidney Allocation System (KAS) improved the rates of transplantation in highly sensitized (HS) patients. However, a subset of HS patients remain at a…
2022 American Transplant Congress
Production of Exosomal IL35 from Alloreactive Treg Cells: A Basis for Potent Immunotherapy and Tolerance in Human Kidney Transplantation
W. J. Burlingham¹, E. Jankowska-Gan², J. Fechner¹, C. Little¹, S. Tripathi³, B. Schreiber³, A. Chandraker³, D. Foley⁴
¹Surgery-Transplant Division, University of Wisconsin, Madison, WI, ²Surgery-Transplant Division, University of Wisconsin-Madison, Madison, WI, ³Surgery-Transplant Division, Brigham & Women's Hospital, Boston, MA, ⁴University of Wisconsin, Madison, WI
*Purpose: Treg cells make a variety of immunosuppressive products—cytokines such as IL10, TGFβ, and IL35, and enzymes such as the CD39/CD73 pair that convert extracellular…
2022 American Transplant Congress
HLA-Specific IgD/CD27 Double Negative B Cell Frequency Assessed by a Novel Multiplex Bead-Based Assay Predicts Rejection in Kidney Transplant Recipients
Z. Ilyas¹, A. Akl², D. Pinelli¹, L. Gallon³, A. Tambur¹, M. Ansari⁴
¹Northwestern University, Chicago, IL, ²Dr. Soliman Fakeeh Hospital, Jeddah, Saudi Arabia, ³Northwestern Univ NMFF, Chicago, IL, ⁴Medicine, Northwestern University, Chicago, IL
*Purpose: Recipients with sensitization to HLA defined by a high percentage of Panel Reactive Antibodies (PRA) or Donor-specific anti-HLA antibodies (DSA) are at higher immunologic…
2022 American Transplant Congress
Stem Cell Donor-Derived CPRA Better Reflects Sensitization Than Current CPRA
K. Lindblad¹, C. Murphey², P. Lalli³, J. Lunz⁴, L. Gragert⁵
¹UNOS, Richmnd, VA, ²Southwest Immunodiagnostics, Inc., San Antonio, TX, ³Carolinas Medical Center, Charlotte, NC, ⁴LifeLink Foundation Transplant Immunology Lab, Tampa, FL, ⁵Tulane University School of Medicine, New Orleans, LA
*Purpose: The OPTN Histocompatibility Committee proposes replacing the current calculated panel reactive antibody (CPRA), based on HLA haplotype frequencies from ~14,000 deceased kidney donors, with…
2022 American Transplant Congress
Concurrent Use of Two Methods Prevents Erroneous HLA Typing of Deceased Organ Donors – An Essential Strategy for Patient Safety and Accurate Virtual Crossmatching for Broader Sharing
R. Rajalingam, T. dela Cruz, C. Dames, L. Pagaduan, Y. Cho, D. Kong
Immunogenetics and Transplantation Laboratory (ITL), University of California San Francisco, San Francisco, CA
*Purpose: Comprehensive and accurate typing for ever-increasing alleles of all eleven HLA genes within a short turnaround time is a crucial initial step for allocating…
2022 American Transplant Congress
Site-Directed Mutagenesis as a Tool to Investigate Immunogenicity of HLA Epitopes for Humoral Alloreactivity
C. H. Maguire¹, P. Crivello², A. Tambur¹
¹Northwestern University, Chicago, IL, ²University Hospital Essen, Essen, Germany
*Purpose: Molecular mismatch load (MML) analysis has been a proposed approach to assess donor/recipient compatibility in support of organ allocation algorithms. While conceptually appealing, MML…
2022 American Transplant Congress
Histocompatibility Findings in the First Xenotransplants from a Pig to Deceased Human Recipient
M. Mangiola, V. Tatapudi, J. Stern, Z. Stewart Lewis, B. Lonze, N. Ali, R. Montgomery
Transplant Institute, NYU Langone Transplant Institute, New York, NY
*Purpose: Humans lack α-1,3-Gal (α-gal) epitopes, and naturally occurring α-gal antibodies (Abs) cause hyperacute rejection of porcine xenografts. We conducted 2 successful xenotransplants in brain-dead…

1
2
3
…
9
Next Page »