Articles tagged "Gene expression"

2018 American Transplant Congress
Increased Expression of HLA-DPB1 in Pre-Implantation Kidney Biopsies is Associated with Poor Late Graft Function
K. Mine,¹ T. Mourão,¹ C. Felipe,² G. Rampim,¹ J. Medina-Pestana,² H. Tedesco-Silva,² M. Gerbase-DeLima.¹
¹Instituto de Imunogenética, AFIP, São Paulo, Brazil; ²Hospital do Rim, Universidade Federal de São Paulo, São Paulo, Brazil.
In a previous gene expression microarray study in pre-implantation biopsies (PIB) from kidneys of 53 deceased donors (GeneChip® Human Gene 1.0 ST Arrays, Affymetrix; GEO…
2018 American Transplant Congress
Results of LITMUS (NCT 02541916): The Liver Immune Tolerance Bio Marker Utilization Study
A. Chruscinski,¹ V. Rojas-Luengas,¹ A. Issacher,¹ J. Luo,⁴ H. Yowanto,⁴ N. Selzner,¹ L. Lilly,¹ R. Smith,¹ E. Renner,¹ J. Zhang,¹ D. Grant,¹ O. Adeyi,¹ H. Atkins,² P. Juni,³ G. Levy.¹
¹Multi Organ Transplant Program, University Health Network, Toronto, ON, Canada; ²The Ottawa Hospital Blood and Marrow Transplant Program, The Ottawa Hospital, Ottawa, ON, Canada; ³Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, ON, Canada; ⁴SCIEX, Brea, CA.
Background: Previously we reported a novel biomarker gene set for the identification of tolerance in murine models of rapamycin-induced cardiac tolerance and spontaneous hepatic tolerance.…
2018 American Transplant Congress
β-Glucuronidase Gene Expression in Stool Samples Associated with Chronic Allograft Dysfunction in Kidney Transplants
A. Israni,^1,2 D. Knights,³ G. Al-Ghalith,³ A. Muthusamy,² B. Wu,⁴ W. Oetting,⁵ D. Schaldt,² R. Remmel,⁵ P. Jacobson,⁵ For DeKAF Genomics & GEN03 Investigators.¹
¹Medicine, HCMC, University of MN (UMN), Minneapolis; ²Minneapolis Medical Research Foundation (MMRF), Mpls; ³Computer Science, UMN, Mpls; ⁴Biostatistics, UMN, Mpls; ⁵College of Pharmacy, UMN, Mpls.
Background: Myocophenolate mofetil (MMF) is a prodrug that is extensively and rapidly converted to the active component, mycophenolic acid (MPA). MPA undergoes extensive enterohepatic recycling…
2018 American Transplant Congress
Intragraft MicroRNAs Foretell Fibrosis in Kidney Transplant Recipients
H. Yang,¹ L. Amrouche,² K. Akat,³ C. Li,¹ Z. Khan,¹ C. Tinel,² T. Tuschl,³ D. Anglicheau,² T. Muthukumar.¹
¹Weill Cornell Medicine, New York; ²Hôpital Necker, Paris, France; ³Rockefeller University, New York.
BackgroundIdentifying biomarkers that anticipate tubulointerstitial fibrosis (IFTA) of the kidney allograft will be a transformative advance.Aim/HypothesisIntragraft microRNA profile of 3-month surveillance biopsies reported as normal…
2018 American Transplant Congress
Novel Approach to Molecular Testing Using Routine Diagnostic Tissue
P. Herzyk,¹ F. McMonagle,¹ P. Shiels,¹ S. Coley.²
¹University of Glasgow, Glasgow, United Kingdom; ²Columbia University, New York.
Currently, molecular diagnostics in kidney transplantation requires dedicated fresh tissue. This requirement limits use in mainstream clinical settings due to perceived increased patient risk in…
2018 American Transplant Congress
mRNA Transcript Profiles of 14 Genes in Plasma Exosome Predict Risk for Antibody-Mediated Rejection (ABMR) of Renal Allografts
H. Zhang,¹ E. Huang,² C. Nast,³ J. Kahwaji,² A. Vo,² S. Jordan,² M. Toyoda.¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA; ²Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA; ³Pathology, Cedars-Sinai Medical Center, Los Angeles, CA.
Background: Previously we reported that mRNA transcript profiles of 4 genes (gp130, SH2D1B, TNFα and CCL4) in plasma exosome could predict the risk of ABMR,…
2018 American Transplant Congress
In-Situ Tissue-Based Gene Expression Profiling is a Reliable Method to Detect Gene Transcripts in Human Kidney Transplant Biopsies
D. Dobi,¹ J. Henrik,² T. Sigdel,² A. Chen,¹ Z. Laszik.¹
¹Department of Pathology, University of California, San Francisco, San Francisco, CA; ²Department of Surgery, University of California, San Francisco, San Francisco, CA.
Purpose. Validation studies that would enable the clinical application of quantitative in situ tissue-based gene expression profiling in renal transplantation are lacking. In this study,…
2018 American Transplant Congress
Modification of Macrophage Phenotype by the Magnetic Field
M. Kloc,¹ W. Chen,¹ J. Kubiak,^2,3 X. Li,¹ R. Ghobrial,¹ J. Wosik.⁴
¹Houston Methodist Research Institute, Houston, TX; ²WIHE, Warsaw, Poland; ³University of Rennes, Rennes, France; ⁴University of Houston, Houston.
Objective. Macrophages play a crucial role in health, disease and chronic rejection of transplanted organs. Chronic rejection of transplanted organs remains unresolved issue in organ…
2018 American Transplant Congress
Molecular Profiling of the Injury-Repair Response in Early Subclinical Inflammation
M. Seifert, D. Della Manna, X. Liu, F. Rosenblum, M. Yanik, E. Yang, J. Pollock, R. Mannon.
UAB School of Medicine, Birmingham.
Purpose: Surveillance biopsies can detect subclinical T-cell mediated rejection (SC-TCMR) or subclinical borderline rejection (SC-B-TCMR) in kidney transplants with normal/stable creatinine. Some consider SC-B-TCMR to…
2018 American Transplant Congress
Bioinformatics Approach to the Development of a Novel Molecular Biomarker for Sub-Clinical Acute Rejection (subAR) in the Peripheral Blood Following Kidney Transplant (KT)
T. Whisenant,¹ S. Kurian,¹ M. Kandpal,² L. Zhao,² D. Ikle,³ B. Armstrong,³ J. Friedewald,² R. Heilman,⁴ E. Poggio,⁵ C. Marsh,¹ P. Baliga,⁶ N. Bridges,⁷ J. Odim,⁷ M. Brown,⁷ J. Charette,² S. Brietigam,² N. Sustento-Reodica,² D. Salomon,¹ M. Abecassis.²
¹Scripps, La Jolla; ²Northwestern, Chicago; ³Rho, Chapel Hill; ⁴Mayo AZ, Scottsdale; ⁵Cleveland Clinic, Cleveland; ⁶MUSC, Charleston; ⁷NIAID, Bethesda.
Sub-clinical acute rejection (subAR) following KT is associated with poor long-term graft outcomes and can only be detected using invasive surveillance biopsies (SBx). Non-invasive biomarker…