Articles tagged "Gene expression"

2019 American Transplant Congress
Endothelial Cells Do Not Regulate Expression of CD46, CD55 and CD59 in the Presence of Anti-HLA Class I and 11 Antibodies
A. Babu¹, T. Pham², M. Hamdorf², V. Jucaud², M. Everly², S. Daga³, D. Mitchell⁴
¹Transplant Institute, Henry Ford Hospitals, Detroit, MI, ²Terasaki Research Institute, Los Angeles, CA, ³University of Warwick, Coventry, United Kingdom, ⁴Medical Sciences, University of Warwick, Coventry, United Kingdom
*Purpose: Anti-HLA antibodies, both pre-formed and de novo are associated with worse graft survival. Complement activation is an important mechanism of antibody mediated immunological injury.…
2019 American Transplant Congress
Immune Cell Types and Host Gene Signatures Associated with Epstein-Barr Virus (EBV) Post-Transplant Lymphoproliferative Disorder (PTLD)
J. Toh¹, F. Vallania¹, W. A. Haynes¹, G. Bhagat², F. E. Craig³, S. H. Swerdlow⁴, T. Tousseyn⁵, S. M. Krams¹, P. Khatri¹, O. M. Martinez¹
¹Stanford University School of Medicine, Stanford, CA, ²Columbia University Medical Center, New York, NY, ³Mayo Clinic College of Medicine, Phoenix, AZ, ⁴University of Pittsburgh School of Medicine, Pittsburg, PA, ⁵Katholieke Universiteit Leuven, Leuven, Belgium
*Purpose: PTLD is a serious post-transplant complication that encompasses a range of abnormal lymphoproliferations, including EBV+ B cell lymphomas. Although many are EBV-driven, a significant…
2019 American Transplant Congress
Outcomes and Clinical Utility of the kSORT Assay in the Prism Prospective Clinical Trial of Highly Sensitized Kidney Transplant Recipients
A. Schroeder, P. Towfighi, C. Hu, S. Hsieh, J. Liberto, I. Damm, T. Sigdel, P. Millar, M. Sarwal, F. Vincenti
Department of Surgery, UCSF, San Francisco, CA
*Purpose: kSORT, a 17 gene blood biomarker, has been retrospectively validated for detection of biopsy proven acute rejection (AR) and immune quiescence in kidney transplantation…
2019 American Transplant Congress
Non-Invasive Monitoring of the Kidney Allograft
S. Bontha¹, G. Darville¹, C. Kuscu¹, C. Kuscu², A. Bajwa¹, J. Eason³, D. Maluf⁴, V. Mas⁵
¹Surgery, UTHSC, Memphis, TN, ²Surgery, UTHSC, memphis, TN, ³Surgery, Director of Transplant Institute - UTHSC, Memphis, TN, ⁴UTHSC, Memphis, TN, ⁵Surgery, Director of Transplant Research Institute, Memphis, TN
*Purpose: A major issue in kidney transplantation is the lack of validated non-invasive biomarkers for monitoring graft function, current biochemical markers (i.e., creatinine) have poor…
2019 American Transplant Congress
Efficacious Home Processing of Urine for Messenger RNA Profiling: A Point-of-Care Approach for Molecular Monitoring of Kidney Transplant Recipients
C. Snopkowski¹, C. Li¹, S. Albakry¹, B. Botticelli¹, M. Cassidy¹, J. Lee¹, M. Lubetzky¹, D. Dadhania¹, H. Yang¹, R. Ding¹, T. Muthukumar¹, M. Suthanthiran²
¹WCM, NYC, NY, ²WCM, New York, NY
*Purpose: Urinary mRNA profiling has been validated in multi-center studies as a robust tool for monitoring kidney transplant recipients. Home processing of urine samples for…
2019 American Transplant Congress
An 8-Gene Expression Assay in Peripheral Blood for Diagnosis of Antibody-Mediated Rejection after Kidney Transplantation
E. Van Loon¹, S. Gazut², S. Yazdani¹, E. Lerut¹, H. de Loor¹, M. Coemans¹, L. Noël³, L. Thorrez¹, L. Van Lommel¹, F. Schuit¹, D. Lambrechts¹, T. Van Brussel¹, B. Sprangers¹, D. Kuypers¹, M. Essig⁴, W. Gwinner⁵, D. Anglicheau⁶, P. Marquet⁷, M. Naesens¹
¹Catholic University Leuven, Leuven, Belgium, ²CEA, Gif-sur-Yvette, France, ³Necker-Enfants Malades Institute, Paris, France, ⁴CHU Limoges, Limoges, France, ⁵Hannover Medical School, Hannover, Germany, ⁶Paris Descartes, Sorbonne Paris Cité University, Paris, France, ⁷RTRS Centaure, Necker Hospital, Paris, France
*Purpose: Antibody-mediated rejection is a leading cause of graft failure after kidney transplantation. The diagnosis of antibody-mediated rejection is made by combining assessment of circulating…
2019 American Transplant Congress
Differentiation and Characterization of Human Endodermal Precursors for Liver Cell Therapies
W. S. Fagg¹, N. Liu¹, M. Shoja¹, I. Patrikeev², M. Motamedi², J. Fair¹
¹Surgery, University of Texas Medical Branch, Galveston, TX, ²Opthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX
*Purpose: Mouse pluripotent stem cell (PSC)-derived endoderm precursors (EPs) have small size, endoderm lineage gene expression signatures, and high proliferation rates, that allow engraftment in…
2019 American Transplant Congress
Absolute Quantification of Micro RNA and Messenger RNA Using a Customized Amplicon in Real Time Quantitative Polymerase Chain Reaction Assays
C. Li, C. Snopkowski, M. Cassidy, B. Botticelli, S. Albakry, J. R. Lee, M. Lubetzky, D. Dadhania, H. Yang, R. Ding, T. Muthukumar, M. Suthanthiran
WCMC, New York, NY
*Purpose: Transcript abundance in biological specimens quantified by RT-PCR assay is typically expressed as fold change between groups. Absolute quantification is preferable to relative quantification…
2019 American Transplant Congress
Borderline Changes in Kidney Allografts Represents a Non-Homogenous Cohort with Different Outcome
P. Hruba¹, J. Maluskova¹, Z. Krejcik², M. Merkerova-Dostalova², V. Stranecky³, J. Klema⁴, K. Osickova¹, M. Wohlfahrtova¹, E. Honsova¹, O. Viklicky¹
¹Institute for Clinical and Experimental Medicine, Prague, Czech Republic, ²Institute of Hematology and Blood Transfusion, Prague, Czech Republic, ³Institute of Inherited Metabolic Disorders, Prague, Czech Republic, ⁴Czech Technical University, Prague, Czech Republic
*Purpose: Borderline changes (BL) represent a frequent histological finding in kidney allograft with unclear prognosis.*Methods: In this single center, retrospective, observational study, the BL were…
2019 American Transplant Congress
A Molecular Analysis of Graft Survival in the INTERHEART Study
P. Halloran¹, J. Reeve and the INTERHEART investigators²
¹University of Alberta, Edmonton AB, AB, Canada, ²ATAGC, University of Alberta, Edmonton AB, AB, Canada
*Purpose: Rejection is a major cause of graft loss in heart and kidney transplants. The principal diagnosis associated with risk in kidneys is antibody-mediated rejection…