Articles tagged "Gene expression"

2019 American Transplant Congress
Subtyping Of Histologically Stable Kidney Transplants In Molecular Meta-analysis
D. Rychkov¹, T. Sigdel², J. Liberto², M. Sarwal², M. Sirota³
¹ICHS, Department of Surgery, UCSF, San Francisco, CA, ²Department of Surgery, UCSF, San Francisco, CA, ³ICHS, UCSF, San Francisco, CA
*Purpose: Histologically stable allografts have been shown to have molecular inflammation by prior gene expression studies. Nevertheless, there are no methodologies in place to sub-group…
2019 American Transplant Congress
Gene Expression Profiling for Cardiac Transplant Recipients: Results from the Outcomes AlloMap® Registry
S. Hall¹, G. Ewald², P. Berman³, A. Kao⁴, T. Wolf-Doty⁵, J. Yee⁵, J. Kobashigawa⁶
¹Baylor University Medical Center, Dallas, TX, ²Washington University, St. Louis, MO, ³Tampa General Hospital, Tampa, FL, ⁴Saint Luke's Mid America Heart Institute, Kansas City, MO, ⁵CareDx, Brisbane, CA, ⁶Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: Transplant registries may facilitate collection of comprehensive and unbiased real-world data on short and long-term outcomes of patients. We share the results of patients…
2019 American Transplant Congress
RNA Splicing and Lineage Progression: Identification of an Integrated Splicing Pattern in Endodermal Lineage Progression
J. Fair¹, N. Liu¹, S. G. Widen², W. S. Fagg¹
¹Surgery, University of Texas Medical Branch, Galveston, TX, ²Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX
*Purpose: Epigenetic states such as histone modifications and DNA methylation that influence transcription are well-characterized regulators of stem cell pluripotency and differentiation to specific lineages.…
2019 American Transplant Congress
The Complexity of T Cell-Mediated Rejection Scenarios: Identifying BK in Biopsies with TCMR
P. F. Halloran and The INTERCOMEX Investigators¹, G. Gupta², J. Reeve³
¹University of Alberta, Edmonton AB, AB, Canada, ²Virginia Commonwealth University, Richmond, VA, ³Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada
*Purpose: Some transplanted kidneys have concomitant T cell-mediated rejection (TCMR) and BK virus nephropathy (BK). The histologic diagnosis in BK sometimes overlooks the TCMR component,…
2019 American Transplant Congress
Cell Coding Transcriptomic Analysis Of Serial Blood And Biopsy Samples In A Large Cohort Of Kidney Transplant Recipients
W. Zhang¹, Z. Yi¹, Z. Sun¹, K. Keung², C. Wei¹, C. Xi¹, S. Farouk¹, M. Menon¹, P. Cravedi¹, P. O'Connell², B. Murphy¹
¹Icahn School of Medicine at Mount Sinai, New York, NY, ²The University of Sydney, Sydney, Australia
*Purpose: Whole blood and graft biopsy RNA expression analyses are limited by the heterogeneous cell composition of the samples. Cell coding is an analytical tool…
2019 American Transplant Congress
Interferon Sensitivity Promotes Recovery from Acetaminophen Induced Liver Injury
S. Datta, T. Hamilton, G. C. Sen
Inflammation and Immunity, Cleveland Clinic Foundation, Cleveland, OH
*Purpose: The current study was undertaken to assess the role of inflammatory signaling during induction of and recovery from acetaminophen induced acute liver failure.*Methods: C57BL/6…
2019 American Transplant Congress
Pluripotent Stem Cell Differentiation: Delaying Forward Differentiation Signals Maintains Definitive Endoderm Phenotype for Cellular Transplantation
J. Fair¹, M. M. Shoja¹, N. Liu¹, I. Patrikeev², M. Motamedi², W. S. Fagg¹
¹Surgery, University of Texas Medical Branch, Galveston, TX, ²Opthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX
*Purpose: Endodermal precursors (EP), derived in vitro from mouse pluripotent stem cell (PSC) sources, are capable of clinically useful engraftment in the unmodified (quiescent) liver…
2019 American Transplant Congress
Single Cell Sequencing of Human Kidney Allograft
H. Yang¹, H. Suryawanshi², M. Lagman¹, M. Lubetzky¹, A. Alonso¹, S. Seshan¹, T. Tuschl², M. Suthanthiran¹, T. Muthukumar¹
¹Weill Cornell Medicine, New York, NY, ²Rockefeller University, New York, NY
*Purpose: Transcriptome-based clustering of individual cells within the kidney allograft may help identify cell type-specific injury and has the potential to redefine the current histopathology-based…
2019 American Transplant Congress
Pre-Transplant Transcriptomic Signature in Peripheral Blood Predicts Early Acute Rejection after Kidney Transplant
W. Zhang¹, Z. Yi¹, C. Wei¹, K. Keung², Z. Sun¹, C. Xi¹, C. Woytovich¹, S. Farouk¹, L. Gallon³, M. Menon¹, C. Magee⁴, N. Najafian⁴, M. Samaniego⁵, A. Djamali⁶, S. Alexander², I. Rosales⁷, R. Smith⁷, P. O'Connell², R. Colvin⁷, P. Cravedi¹, B. Murphy¹
¹Icahn School of Medicine at Mount Sinai, New York, NY, ²The University of Sydney, Sydney, Australia, ³Northwestern University Feinberg School of Medicine, Chicago, IL, ⁴Brigham and Women's Hospital, Boston, MA, ⁵Henry Ford Hospital, Detroit, MI, ⁶University of Wisconsin, Madison, WI, ⁷Massachusetts General Hospital, Boston, MA
*Purpose: Early development of acute rejection after kidney transplantation is associated with diminished long-term graft survival. Predicting early acute rejection (EAR) at the time of…
2019 American Transplant Congress
Transcriptional Changes in PBMC Associated with Development of Infection and Rejection in Older and Younger Kidney Transplant Recipients
J. Schaenman¹, M. Rossetti², E. Liang¹, O. E. Beaird¹, S. Bunnapradist³, E. F. Reed², S. Cole⁴
¹Division of Infectious Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, ²Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, ³Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, CA, ⁴Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA
*Purpose: Changes in immune response are central to vulnerability to infection and rejection, especially for the growing numbers of older kidney transplant recipients. We sought…