Articles tagged "Gene expression"

2019 American Transplant Congress
Differentiation and Characterization of Human Endodermal Precursors for Liver Cell Therapies
W. S. Fagg¹, N. Liu¹, M. Shoja¹, I. Patrikeev², M. Motamedi², J. Fair¹
¹Surgery, University of Texas Medical Branch, Galveston, TX, ²Opthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX
*Purpose: Mouse pluripotent stem cell (PSC)-derived endoderm precursors (EPs) have small size, endoderm lineage gene expression signatures, and high proliferation rates, that allow engraftment in…
2019 American Transplant Congress
Absolute Quantification of Micro RNA and Messenger RNA Using a Customized Amplicon in Real Time Quantitative Polymerase Chain Reaction Assays
C. Li, C. Snopkowski, M. Cassidy, B. Botticelli, S. Albakry, J. R. Lee, M. Lubetzky, D. Dadhania, H. Yang, R. Ding, T. Muthukumar, M. Suthanthiran
WCMC, New York, NY
*Purpose: Transcript abundance in biological specimens quantified by RT-PCR assay is typically expressed as fold change between groups. Absolute quantification is preferable to relative quantification…
2019 American Transplant Congress
Borderline Changes in Kidney Allografts Represents a Non-Homogenous Cohort with Different Outcome
P. Hruba¹, J. Maluskova¹, Z. Krejcik², M. Merkerova-Dostalova², V. Stranecky³, J. Klema⁴, K. Osickova¹, M. Wohlfahrtova¹, E. Honsova¹, O. Viklicky¹
¹Institute for Clinical and Experimental Medicine, Prague, Czech Republic, ²Institute of Hematology and Blood Transfusion, Prague, Czech Republic, ³Institute of Inherited Metabolic Disorders, Prague, Czech Republic, ⁴Czech Technical University, Prague, Czech Republic
*Purpose: Borderline changes (BL) represent a frequent histological finding in kidney allograft with unclear prognosis.*Methods: In this single center, retrospective, observational study, the BL were…
2019 American Transplant Congress
A Molecular Analysis of Graft Survival in the INTERHEART Study
P. Halloran¹, J. Reeve and the INTERHEART investigators²
¹University of Alberta, Edmonton AB, AB, Canada, ²ATAGC, University of Alberta, Edmonton AB, AB, Canada
*Purpose: Rejection is a major cause of graft loss in heart and kidney transplants. The principal diagnosis associated with risk in kidneys is antibody-mediated rejection…
2019 American Transplant Congress
Organ Resilience Contributes to Different Impact of Delayed Graft Function (DGF) on Graft Survival in Kidneys Donated by Brain Death (DBD) and Circulatory Death Donors (DCD)
M. J. de Kok, A. F. Schaapherder, L. G. Wijermars, D. K. de Vries, J. L. Lindeman
Transplantation Center, LUMC, Leiden, Netherlands
*Purpose: Despite a tripled higher incidence of DGF in DCD kidney grafts compared to DBD kidney grafts, large studies show equivalent long-term graft survival with…
2019 American Transplant Congress
RNA Sequencing Identifies a Differential Role of Coding and Non-Coding Genes by Types of Rejection
S. Pineda¹, O. Bestard², T. K. Sigdel¹, M. Sirota³, M. Sarwal¹
¹Department of Surgery, UCSF, San Francisco, CA, ²Department of Surgery, Bellvitge University Hospital, Barcelona, Spain, ³BCHSI, UCSF, San Francisco, CA
*Purpose: The differentiation of histological phenotypes of T cell mediated cellular rejection (TCMR) and humoral or antibody mediated rejection (AMR) of kidney allografts, bears relevance…
2019 American Transplant Congress
The Molecular Pathology of Immune Checkpoint Inhibitor-Associated Renal Allograft Rejection: Overlap with Acute Interstitial Nephritis
B. Adam¹, C. Boils², L. Bu³, K. Renaudin⁴, K. Wen¹, M. Mengel¹
¹University of Alberta, Edmonton, AB, Canada, ²Arkana Labs, Little Rock, AR, ³University of Minnesota, Minneapolis, MN, ⁴CHU Nantes, Nantes, France
*Purpose: The use of immune checkpoint inhibitors (ICIs) for various malignancies has been associated with numerous immunological adverse events, including acute interstitial nephritis (AIN) in…
2019 American Transplant Congress
Results of Litmus (nct 02541916): The Liver Immune Tolerance Bio Marker Utilization Study
A. Chruscinski¹, V. Rojas-Luengas¹, S. Moshkelgosha¹, J. Luo², H. Yowanto², Z. Galvin¹, L. Lilly¹, R. Smith¹, E. Renner¹, J. Zhang¹, D. Grant¹, O. Adeyi¹, H. Atkins³, P. Juni⁴, N. Selzner¹, S. Juvet¹, G. Levy¹
¹Multi-Organ Transplantation, University Health Network, Toronto, ON, Canada, ²Sciex, Brea, CA, ³Ottawa Hospital Research Institute, Ottawa, ON, Canada, ⁴University of Toronto, Toronto, ON, Canada
*Purpose: Previously we reported a novel biomarker gene set for the identification of tolerance in murine models of rapamycin-induced cardiac tolerance and spontaneous hepatic tolerance.…
2019 American Transplant Congress
Molecular Microscope Proves i-IFTA Lack of Specificity
A. Perkowska-Ptasinska¹, M. Ciszek², D. Deborska-Materkowska¹, A. Debska-Slizien³, P. F. Halloran⁴, M. Myslak⁵
¹Warsaw Medical Univeristy, Warsaw, Poland, ²Department of Immunology, Transplantology and Internal Diseases, Warsaw Medical Univeristy, Warsaw, Poland, ³Gdansk Medical University, Gdansk, Poland, ⁴ATAGC, University of Alberta, Edmonton AB, AB, Canada, ⁵Pomeranian Medical University, Szczecin, Poland
*Purpose: According to 2017 Banff classification, inflammatory infiltrate in the fibrotic renal tissue with at least moderate total inflammations score (ti≥2), and tubulitis (t≥2) should…
2019 American Transplant Congress
Validation of Gene Expression Signatures Associated with Antibody-Mediated Rejection (ABMR) in Renal Allograft Biopsies (Bx) of HLA-Sensitized Patients
H. Zhang¹, C. C. Nast², N. Ammerman³, A. A. Vo³, S. C. Jordan³, M. Toyoda¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, ²Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: "Thoroughly validated gene transcripts” in renal Bx was adopted as one of the criteria for ABMR diagnosis in the latest Banff classification. Previously, we…