Articles tagged "Gene expression"

2019 American Transplant Congress
Molecular Microscope Proves i-IFTA Lack of Specificity
A. Perkowska-Ptasinska¹, M. Ciszek², D. Deborska-Materkowska¹, A. Debska-Slizien³, P. F. Halloran⁴, M. Myslak⁵
¹Warsaw Medical Univeristy, Warsaw, Poland, ²Department of Immunology, Transplantology and Internal Diseases, Warsaw Medical Univeristy, Warsaw, Poland, ³Gdansk Medical University, Gdansk, Poland, ⁴ATAGC, University of Alberta, Edmonton AB, AB, Canada, ⁵Pomeranian Medical University, Szczecin, Poland
*Purpose: According to 2017 Banff classification, inflammatory infiltrate in the fibrotic renal tissue with at least moderate total inflammations score (ti≥2), and tubulitis (t≥2) should…
2019 American Transplant Congress
Validation of Gene Expression Signatures Associated with Antibody-Mediated Rejection (ABMR) in Renal Allograft Biopsies (Bx) of HLA-Sensitized Patients
H. Zhang¹, C. C. Nast², N. Ammerman³, A. A. Vo³, S. C. Jordan³, M. Toyoda¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, ²Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: "Thoroughly validated gene transcripts” in renal Bx was adopted as one of the criteria for ABMR diagnosis in the latest Banff classification. Previously, we…
2019 American Transplant Congress
Role of Molecular Microscope in the Interpretation of Endarteritis and Acute Thrombotic Microangiopathy
M. Ciszek¹, A. Perkowska-Ptasińska¹, D. Deborska-Materkowska¹, A. Debska-Slizien², P. F. Halloran³, M. Myslak⁴
¹Warsaw Medical Univeristy, Warsaw, Poland, ²Gdansk Medical University, Gdansk, Poland, ³ATAGC, University of Alberta, Edmonton AB, AB, Canada, ⁴Pomeranian Medical University, Szczecin, Poland
*Purpose: According to the Banff classification, endarteritis (Figure 1A, case 1) may be a manifestation of either: TCMR and ABMR. Acute thrombotic microangiopathy (TMA) constitutes…
2019 American Transplant Congress
Decreased Cancer Susceptibility in Tolerant Liver Transplant Recipients: Another Benefit of Tolerance?
M. P. Bhat¹, E. Pasini¹, C. Baciu¹, S. M. Kurian², J. Levitsky³
¹Multi-Organ Transplant, UHN, Toronto, ON, Canada, ²Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, ³Division of Gastroenterology & Hepatology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL
*Purpose: Long-term survival of transplant recipients is significantly impacted by malignancy. Induction of tolerance is feasible in a subset of liver transplant (LT) recipients. The…
2019 American Transplant Congress
Single Cell Analysis of Kidney Allograft Rejection
C. M. Castro-Rojas¹, K. M. Roskin², C. G. Moore², B. Abu Jawdeh³, N. Salomonis², R. R. Alloway⁴, E. Woodle⁴, D. A. Hildeman¹
¹Immunobiology, Cincinnati Children's Hospital, Cincinnati, OH, ²Biomedical Informatics, Cincinnati Children's Hospital, Cincinnati, OH, ³Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, ⁴Transplantation, University of Cincinnati College of Medicine, Cincinnati, OH
*Purpose: Rejection therapies (corticosteroids and antilymphocyte globulins) have not changed in over 50 years. In part this is due to a knowledge deficit of signaling…
2019 American Transplant Congress
Molecular Phenotype of Proliferative Arteriopathy in Kidney Grafts
A. Perkowska-Ptasinska¹, M. Ciszek¹, D. Deborska-Materkowska¹, A. Debska-Slizien², P. F. Halloran³, M. Myslak⁴
¹Warsaw Medical University, Warsaw, Poland, ²Gdansk Medical University, Gdansk, Poland, ³University of Alberta, Edmonton AB, AB, Canada, ⁴Pomeranian Medical University, Szczecin, Poland
*Purpose: Proliferative arteriopathy (PA) is a lesion characterized by a fibrotic tissue in the arterial intima. The phenomenon is marked by the lack of elastic…
2019 American Transplant Congress
Correlation between Gene Expression and Histology in Transbronchial Lung Transplant Biopsies
B. Adam, K. Du, S. Rotich, M. Mengel
University of Alberta, Edmonton, AB, Canada
*Purpose: Transbronchial lung transplant biopsies (TBBx) are limited by non-specific histologic lesions and suboptimal interobserver reproducibility. Gene expression offers a potentially more precise, objective and…
2019 American Transplant Congress
The Role of CD47/SIRPα and CD47/TSP-1 Pathways in Pig-to-Baboon Kidney Transplantation Using hCD47 Transgenic Kidneys
K. Takeuchi¹, Y. Ariyoshi¹, T. Pomposelli¹, L. Boyd¹, D. Ekanayake-Alper¹, S. Arn¹, R. Hawley¹, D. Ayares², M. Lorber³, M. Sykes¹, D. Sachs¹, R. Johnson⁴, K. Yamada¹
¹CCTI/Surgery, Columbia University Medical Center, New York, NY, ²Revivicor Inc., Blacksburg, VA, ³Lung BioTechnology PBC, Silver Spring, MD, ⁴Division of Renal Diseases and Hypertension, University of Colorado, Aurora, CO
*Purpose: We have recently reported that both CD80 upregulation and the degradation of SMPDL-3b on glomerular podocytes are involved in the development of post kidney…
2019 American Transplant Congress
Integrative Analysis of Immune Repertoire and Gene Expression from RNA-seq in Kidney Organ Transplants
S. Pineda¹, A. Ravoor², O. Bestard³, M. Sarwal¹, M. Sirota²
¹Department of Surgery, UCSF, San Francisco, CA, ²BCHSI, UCSF, San Francisco, CA, ³Department of Surgery, 3Bellvitge University Hospital, Barcelona, Spain
*Purpose: The main problem with kidney transplantation is the possibility of rejection, which is classified as either antibody-mediated rejection (AMR) or T-cell-mediated rejection (TCMR) depending…
2019 American Transplant Congress
Automated Histology Lesion Interpretation in Kidney Transplant Biopsies Shows That Pathologists Often Deviate from Banff Guidelines
K. S. Madill-Thomsen¹, J. Reeve², G. Bohmig³, F. A. Eskandary⁴, A. Perkowska-Ptasinska⁵, M. Myslak⁶, P. F. Halloran and the INTERCOMEX Study Group¹
¹University of Alberta, Edmonton AB, AB, Canada, ²Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ³Medical University of Vienna, Vienna, Austria, ⁴Medical University of Vienna, Edmonton AB, AB, Canada, ⁵Medical University of Warsaw, Warsaw, Poland, ⁶Pomeranian Medical University, Szczecin, Poland
*Purpose: Histologic diagnosis of kidney transplant biopsies requires an expert pathologist using Banff guidelines to interpret features. Some problematic guidelines require experts to use professional…