Articles tagged "Gene expression"

2020 American Transplant Congress
Gene Expression Dynamics Demonstrate Perturbations in Innate and Adaptive Immunity in Kidney Transplantation Patients with Cytomegalovirus Viremia
R. Ahn, J. Schaenman, Z. Qian, V. Groysberg, M. Rossetti, A. Hoffmann, D. Gjertson, M. Deng, E. F. Reed
University of California Los Angeles, Los Angeles, CA
*Purpose: Cytomegalovirus (CMV) viremia and disease continues to cause significant morbidity and mortality in kidney transplant patients with clinical complications including organ rejection and death.…
2020 American Transplant Congress
In Silico Deconvolution of Whole Blood RNA-Seq Data from Iscalimab Phase IIa Study in Renal Transplant Reveals Insights Into Mechanism of Action and Superiority of Iscalimab Compared to Tacrolimus
J. S. Rush
Autoimmunity, Transplantation and Inflammation, Novartis Institutes for Biomedical Research, Basel, Switzerland
*Purpose: A recent Phase 2 clinical study indicated that iscalimab, a pathway blocking, non-depleting anti-CD40 antibody, showed non-inferiority on a composite clinical endpoint, and improved…
2020 American Transplant Congress
Genome-Wide Transcriptome Analysis of Extreme Marginal Renal Grafts Indicates Earlier Repair and Less Damage Following Normothermic Ex-Vivo Kidney Perfusion Preservation
P. Urbanellis¹, L. Mazilescu¹, C. McEvoy¹, S. Ganesh¹, R. John¹, I. Mucsi¹, A. Ghanekar¹, D. Bagli², A. Konvalinka¹, L. Robinson¹, M. Selzner¹
¹University Health Network, Toronto, ON, Canada, ²The Hospital for Sick Children, Toronto, ON, Canada
*Purpose: Normothermic ex-vivo kidney perfusion (NEVKP) was shown to prevent delayed graft function of kidneys subjected to prolonged warm ischemia (WI) compared to static cold…
2020 American Transplant Congress
Single Cell RNA Transcriptomics to Further the Precision Transplantation Medicine Approaches in Kidney Allograft Recipients
H. Suryawanshi, H. Yang, M. Lubetzky, S. Seshan, T. Tuschl, M. Suthanthiran, T. Muthukumar
Weill Cornell Medicine, New York, NY
*Purpose: Test the hypothesis that single cell RNA transcriptomics will further the precision transplantation medicine approaches and address important conundrums in clinical transplantation.*Methods: We studied…
2020 American Transplant Congress
Integrated Multicohort Analysis of Transcriptome Profiles Identifies Blood Gene Signature to Distinguish Rejection from Infection in Patients with Solid Organ Transplants
L. C. Murphy, A. Rao, M. Donato, P. Khatri
School of Medicine, Stanford University, Stanford, CA
*Purpose: Allograft rejection and infection are now leading causes of morbidity and mortality in transplant recipients. Current diagnosis relies on nonspecific markers of graft injury,…
2020 American Transplant Congress
Transcriptomic Profiling of Paired Serum and Biopsy Samples from FSGS Patients
C. Kuscu¹, M. Kiran², A. Wolen¹, C. Kuscu¹, A. Bajwa¹, J. Eason¹, D. Maluf¹, E. Akalin³, V. Mas¹
¹Transplant Research Institute, James D. Eason Transplant Institute, Department of Surgery, University of Tennessee Health Science Center, Memphis, TN, ²Department of Systems and Computational Biology, University of Hyderabad, Hyderabad, India, ³Department of Medicine and Surgery, Albert Einstein College of Medicine, New York, NY
*Purpose: Pathogenesis and molecular footprints of focal segmental glomerulosclerosis (FSGS) after kidney transplantation whether it is primary or secondary is unknown. Herein, we studied a…
2020 American Transplant Congress
Identification of Molecular Markers for Liver Cirrhosis (NASH) by Single-Nucleus RNA Sequencing
C. Kuscu¹, A. Mouhammed², C. Kuscu³, A. R. Wolen³, J. D. Eason³, D. Maluf³, V. Mas³
¹Dept of Surgery, Transplant Research Institute, James D. Eason Transplant Institute, University of Tennessee Health Science Center, Memphis, TN, ²Bioinformatics, University of Tennessee Health Science Center, Memphis, TN, ³Department of Surgery, University of Tennessee Health Science Center, Memphis, TN
*Purpose: Non-alcoholic fatty liver disease (NAFLD) is a progressive disease and it constitutes a significant portion of the chronic liver diseases that affected more than…
2020 American Transplant Congress
The Transcriptomic Analysis Revealed a Clear Deregulation of the C1QB Gene in Peripheral Blood Mononuclear Cells of Renal Transplant Patients with Chronic Antibody Mediated Rejection
G. Zaza¹, S. Granata¹, G. Santoro¹, C. Caletti¹, P. Pontrelli², F. Rascio³, M. Rossi¹, S. Andrighetto¹, G. Grandaliano⁴
¹Department of Medicine, Renal Unit, University/Hospital of Verona, Verona, Italy, ²Department of Emergency and Organ Transplantation-Nephrology Unit, University of Bari, Bari, Italy, ³Nephrology, Dialysis and Transplantation Unit, Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy, ⁴Università Cattolica del Sacro Cuore, Roma, Italy
*Purpose: Chronic antibody-mediated rejection (CAMR) is a multi-factorial pathological condition that can affect more that 40-50% of kidney transplanted recipients. Its clinical evolution is often…
2020 American Transplant Congress
CD4+ T-Cells Express High Levels of Messenger RNA Coding for Heparanase in Cultures with Matrigel
N. Chai, G. Wu, I. Kim, S. Jordan, A. Klein, T. Brennan
Cedars-Sinai Med Ctr, Los Angeles, CA
*Purpose: Heparanase (Hpse) is the sole heparan sulfate (HS) degrading enzyme regulating multiple biological activities that enhance immune cell migration, blood vessel growth and cell…
2020 American Transplant Congress
Donor Derived Cell Free DNA (dd-cfDNA) and Gene Expression Signatures of Antibody-Mediated Rejection (ABMR) May Improve Accuracy of ABMR Diagnosis (Dx)
H. Zhang¹, C. C. Nast², E. Huang³, N. Ammerman³, A. A. Vo³, S. C. Jordan³, M. Toyoda¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, ²Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: Dd-cfDNA has a clinically meaningful association with early Dx of ABMR. Here, we evaluated dd-cfDNA measured prior to for-cause biopsies (Bx), and also measured…