ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Genome-Wide Transcriptome Analysis of Extreme Marginal Renal Grafts Indicates Earlier Repair and Less Damage Following Normothermic Ex-Vivo Kidney Perfusion Preservation

P. Urbanellis1, L. Mazilescu1, C. McEvoy1, S. Ganesh1, R. John1, I. Mucsi1, A. Ghanekar1, D. Bagli2, A. Konvalinka1, L. Robinson1, M. Selzner1

1University Health Network, Toronto, ON, Canada, 2The Hospital for Sick Children, Toronto, ON, Canada

Meeting: 2020 American Transplant Congress

Abstract number: D-280

Keywords: Gene expression, Kidney transplantation, Machine preservation, Warm ischemia

Session Information

Date: Saturday, May 30, 2020

Session Name: Poster Session D: Ischemia Reperfusion & Organ Rehabilitation

Session Time: 3:15pm-4:00pm

 Presentation Time: 3:30pm-4:00pm

Location: Virtual

Related Abstracts
  • Normothermic Ex-Vivo Kidney Perfusion Improves Function of Extreme Marginal Renal Grafts Subjected to Prolonged Ischemia.
  • Normothermic Ex-Vivo Kidney Perfusion Preservation Consistently Improves Extreme Marginal Graft Function Compared to Hypothermic Machine Perfusion

*Purpose: Normothermic ex-vivo kidney perfusion (NEVKP) was shown to prevent delayed graft function of kidneys subjected to prolonged warm ischemia (WI) compared to static cold storage (SCS). To identify mechanisms for this finding, we analyzed the genomic profile of these grafts following storage to that of unmanipulated-naïve kidneys.

*Methods: Kidneys from 30kg-Yorkshire pigs were removed following 120min of WI and subjected to 8hrs of SCS or NEVKP. Kidney biopsies were collected on POD3 and gene expression was compared with naïve porcine kidneys utilizing the Affymetrix GeneChip® Porcine Gene 1.0 ST Array.

*Results: During NEVKP perfusion, 120min WI-grafts demonstrated favourable perfusion characteristics including lactate clearance (0hr:10.48±0.93mmol/L vs 7hr:1.48±0.85mmol/L,p<0.01,n=5), decreasing intra-renal resistance (0hr:2.26±0.9mmHg/mL/min vs 7hr:0.37±0.6mmHg/mL/min,p<0.01,n=5), and urine production. Post-transplantation, 120min WI-grafts with NEVKP, compared to SCS, demonstrated significantly decreased serum creatinine peak values (POD4:12.62±2.34mg/dl vs POD5:18.95±1.11mg/dL,p=0.001) and higher creatinine clearance (POD4:6.61±4.03mL/min vs 0.35±0.30mL/min,p=0.02). Comparison of POD3 transcript expression levels of 120min WI-grafts subjected to NEVKP or SCS to naïve kidneys showed significant alterations in 5703 and 4699 genes, respectively (>±2-fold changes in expression over naïve,n=3,p<0.05, FDR<0.05). Genes related to regeneration and inflammation were among the most highly over expressed in SCS grafts with metabolism-associated genes highly downregulated (table 1). In contrast, genes related to regeneration were less elevated following NEVKP (table 2).

*Conclusions: Grafts damaged by prolonged WI highly over-express genes associated with regeneration. Lower levels of these genes in NEVKP with preserved metabolism-related genes likely correlates to earlier graft repair and/or less damage during storage.

 border=

 border=

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Urbanellis P, Mazilescu L, McEvoy C, Ganesh S, John R, Mucsi I, Ghanekar A, Bagli D, Konvalinka A, Robinson L, Selzner M. Genome-Wide Transcriptome Analysis of Extreme Marginal Renal Grafts Indicates Earlier Repair and Less Damage Following Normothermic Ex-Vivo Kidney Perfusion Preservation [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/genome-wide-transcriptome-analysis-of-extreme-marginal-renal-grafts-indicates-earlier-repair-and-less-damage-following-normothermic-ex-vivo-kidney-perfusion-preservation/. Accessed March 6, 2021.

« Back to 2020 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Home
  • Search
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.