ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "FACS analysis"

  • 2015 American Transplant Congress

    Interleukin-1 Receptor Signaling by Graft Stromal Cells Regulates Donor-Reactive CD8 T Cell Responses to Cardiac Allografts

    S. Iida,1 D. Kish,1 R. Abe,2 K. Tanabe,3 W. Baldwin,1 R. Fairchild.1

    1Immunology, Cleveland Clinic, Cleveland, OH; 2Immunology, Science University of Tokyo, Chiba, Japan; 3Urology, Tokyo Women's Medical University, Tokyo, Japan.

    Allograft reperfusion induces inflammation that directs the infiltration of memory T cells into the allograft and their activation to express functions mediating graft injury. The…
  • 2015 American Transplant Congress

    Increased Precentage of Circulating CD16+ Monocytes Before Transplantation Are Associated With the Occurence of Acute Rejection

    T. van den Bosch,1 L. Hilbrands,2 M. Kraaij,1 E. Vereyken,1 P. Leenen,3 D. Hesselink,1 C. Baan,1 A. Rowshani.1

    1Department of Internal Medicine, section Nephrology and Kidney Transplantation, Erasmus Medical Center, Rotterdam, Netherlands; 2Department of Nephrology, Radboud University Nijmegen Medical Center, Nijmegen, Netherlands; 3Department of Immunology, Erasmus Medical Center, Rotterdam, Netherlands.

    Introduction: Monocytes compose a neglected immune cell type in transplantation despite their contribution to antibody mediated rejection. We aimed to define the phenotypic and functional…
  • 2015 American Transplant Congress

    Detection of Donor Specific HLA-Ab (DSA) Against Native HLA Molecules Expressed On Donor Cell Surface to Eliminate False Positive DSA in Luminex Single Antigen Beads (LMX-SAB)

    G. Chen, L. Lin, D. Tyan.

    Pathology, Stanford University, Palo Alto, CA.

    Introduction:The LMX-SAB has been extensively used for DSA virtual crossmatch (VXM) to predict actual crossmatch (AXM). The extreme pH and harsh chemical reaction conditions used…
  • 2015 American Transplant Congress

    A Novel DSA Flow Cytometer Crossmatch (DSA-FXM) to Eliminate Ambiguous Auto- and Allo-FXM Results

    G. Chen, L. Lin, D. Tyan.

    Pathology, Stanford University, Palo Alto, CA.

    Introduction:Though usually rejected on theoretical grounds, a positive Auto-FXM could result from either or both auto-reactive non-HLA or HLA-antibodies (Ab). Furthermore, a positive Auto-FXM serum…
  • 2015 American Transplant Congress

    Novel Flow Cytometry Method for Detecting Complement-Fixing Blood Group IgG Antibody in ABO-Incompatible Renal Transplantation

    T. Ishizuka,1 M. Yasuo,1 Y. Ishida,1 H. Miura,1 K. Iwadoh,2 T. Murakami,2 K. Kitajima,2 I. Nakajima,2 S. Fuchinoue.2

    1Transplant Immunology, Tokyo Women's Medical University, Shinjuku ward, Tokyo, Japan; 2Surgery III, Tokyo Women's Medical University, Shinjuku ward, Tokyo, Japan.

    【Background】We experienced a hyperacute rejection (HAR) in ABO-incompatible renal transplantation (ABO-I RTx) on POD 0. We hypothesized that complement-fixing IgG anti-A/B blood group antibody (anti-A/B…
  • 2015 American Transplant Congress

    Long-Term Persistence of Innate Lymphoid Cells in the Gut After Intestinal Transplantation

    J. Weiner, J. Zuber, B. Shonts, S. Yang, M. Martinez, T. Kato, M. Sykes.

    Columbia Center for Translational Immunology, Columbia University Medical Center, New York, NY.

    Introduction: Little is known about innate lymphoid cell (ILC) populations in the human gut, and the turnover of these cells and their subsets after transplantation…
  • 2015 American Transplant Congress

    Immune Phenotyping of Peripheral Blood Samples from the CTOT-10 Belatacept Study

    Y. Suessmuth,1 L. Stempora,1 B. Johnson,1 J. Cheeseman,1 Y. Morrison,4 N. Bridges,4 D. Ikle,5 S. Tomlanovich,3 P. Stock,3 R. Mannon,2 K. Newell,1 C. Larsen,1 A. Mehta.1

    1Emory Transplant Center, Atlanta; 2University of Alabama, Birmingham; 3University of California San Francisco, San Francisco; 4NIAID/ NIH, Bethesda; 5Rho, Chapel Hill, NC.

    The purpose of the CTOT-10 study is to evaluate belatacept as maintenance therapy, while minimizing calcineurin inhibitors and corticosteroids in renal transplantation. We designed mechanistic…
  • 2015 American Transplant Congress

    Single Cell Phospho-Protein Analysis Demonstrates That Immunosuppressive Drugs Inhibit MAPK and mTOR Signaling Molecules in Primary Monocytes After Transplantation

    N. Kannegieter, D. Hesselink, R. Kraaijeveld, G. de Graav, M. Betjes, W. Weimar, C. Baan.

    Nephrology and Transplantation, Dept. of Internal Medicine, Erasmus MC, Rotterdam, Netherlands.

    Monocytes have been identified as key players driving rejection processes. It is therefore surprising that there are almost no data available about the impact of…
  • 2015 American Transplant Congress

    Impact of CXCR4/CXCL12 Blockade on Normal Plasma Cells In Vivo

    N. Moore,1 M. Moreno Gonzales,2 M. Stegall.1

    1Department of Surgery, Division of Transplant Surgery and Department of Immunology, Mayo Clinic, Rochester, MN; 2Department of Surgery, Division of Transplant Surgery, Mayo Clinic, Rochester, MN.

    Receptor/ligand interactions in stromal microenvironments play important roles in the localization, development and survival of normal antibody-secreting plasma cells. The goal of the current study…
  • 2015 American Transplant Congress

    Alterations in Peripheral Blood B Cell Subpopulations in Kidney Transplant Recipients With DSA

    B. Ramaswami,1 R. Ippolito,2 B. Elinoff,1 M. Marrari,3 K. Hadi,1 C. Macedo,1 D. Metes,1 A. Shields,4 R. Alloway,5 A. Zeevi,3 F. Lakkis,1 E. Woodle,4 G. Chalasani.1,2

    1Surgery & STI(1), Medicine(2), Pathology(3), University of Pittsburgh, Pittsburgh; 2Surgery(4) & Medicine(5), University of Cincinnati College of Medicine, Cincinnati.

    Peripheral blood B cell subpopulations were assessed over time (1, 3, 6, 9, and 12 months) in living donor kidney transplant recipients undergoing thymoglobulin (n…
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