ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "FACS analysis"

  • 2015 American Transplant Congress

    Comprehensive Study of 3 Nomenclatures to Discriminate CD8 Subsets in Healthy Volunteers and in Kidney Transplant Recipients

    M. Yap,1,3 G. Tilly,1,3 M. Giral,1,2 S. Brouard,1,2 N. Degauque.1,2

    1UMR 1064, INSERM, Nantes, France; 2ITUN, CHU de Nantes, Nantes, France; 3Faculté de Médecine, Université de Nantes, Nantes, France.

    Objective. Historical classification to distinguish central memory (CM), naïve, effector memory (EM), and terminally-differentiated effector memory (TEMRA) CD8 T cells is based on CD45RA and…
  • 2015 American Transplant Congress

    Intra-Renal Cytokine Measurement Detected During Kidney Allograft Biopsies Portend Severity of Histological Injury

    N. Leca, S. Anderson, K. Muczynski.

    Division of Nephrology, University of Washington, Seattle, WA.

    Histological changes observed on renal allograft biopsies, although considered the current standard for evaluation of allograft injury, lack specificity and disease activity assessment. We devised…
  • 2015 American Transplant Congress

    Characterization of a Nude Mouse Model of Allosensitization Conditioned by Adoptive Transfer of Follicular T Helper Cells

    I. Kim, N.-N. Chai, S. Jordan, A. Klein, G. Wu.

    Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA.

    Background Follicular T helper (Tfh) cells play a critical role in follicular B cell activation and maturation during antibody responses to allograft. Here we report…
  • 2015 American Transplant Congress

    Kidney Plasmacytoid Dendritic Cells Display Distinct Cell Surface Markers and May Explain the Induction of Tolerance by Kidney Allografts

    N. Oh, M. Tonsho, D. Ndishabandi, R. Colvin, J. Madsen, A. Alessandrini.

    Massachusetts General Hospital, Boston, MA.

    Achieving tolerance in heart allografts is an important area of research as the need for heart transplantations increase. Recent studies in non-human primates (NHPs) have…
  • 2015 American Transplant Congress

    Interleukin-1 Receptor Signaling by Graft Stromal Cells Regulates Donor-Reactive CD8 T Cell Responses to Cardiac Allografts

    S. Iida,1 D. Kish,1 R. Abe,2 K. Tanabe,3 W. Baldwin,1 R. Fairchild.1

    1Immunology, Cleveland Clinic, Cleveland, OH; 2Immunology, Science University of Tokyo, Chiba, Japan; 3Urology, Tokyo Women's Medical University, Tokyo, Japan.

    Allograft reperfusion induces inflammation that directs the infiltration of memory T cells into the allograft and their activation to express functions mediating graft injury. The…
  • 2015 American Transplant Congress

    Increased Precentage of Circulating CD16+ Monocytes Before Transplantation Are Associated With the Occurence of Acute Rejection

    T. van den Bosch,1 L. Hilbrands,2 M. Kraaij,1 E. Vereyken,1 P. Leenen,3 D. Hesselink,1 C. Baan,1 A. Rowshani.1

    1Department of Internal Medicine, section Nephrology and Kidney Transplantation, Erasmus Medical Center, Rotterdam, Netherlands; 2Department of Nephrology, Radboud University Nijmegen Medical Center, Nijmegen, Netherlands; 3Department of Immunology, Erasmus Medical Center, Rotterdam, Netherlands.

    Introduction: Monocytes compose a neglected immune cell type in transplantation despite their contribution to antibody mediated rejection. We aimed to define the phenotypic and functional…
  • 2015 American Transplant Congress

    Detection of Donor Specific HLA-Ab (DSA) Against Native HLA Molecules Expressed On Donor Cell Surface to Eliminate False Positive DSA in Luminex Single Antigen Beads (LMX-SAB)

    G. Chen, L. Lin, D. Tyan.

    Pathology, Stanford University, Palo Alto, CA.

    Introduction:The LMX-SAB has been extensively used for DSA virtual crossmatch (VXM) to predict actual crossmatch (AXM). The extreme pH and harsh chemical reaction conditions used…
  • 2015 American Transplant Congress

    A Novel DSA Flow Cytometer Crossmatch (DSA-FXM) to Eliminate Ambiguous Auto- and Allo-FXM Results

    G. Chen, L. Lin, D. Tyan.

    Pathology, Stanford University, Palo Alto, CA.

    Introduction:Though usually rejected on theoretical grounds, a positive Auto-FXM could result from either or both auto-reactive non-HLA or HLA-antibodies (Ab). Furthermore, a positive Auto-FXM serum…
  • 2015 American Transplant Congress

    Novel Flow Cytometry Method for Detecting Complement-Fixing Blood Group IgG Antibody in ABO-Incompatible Renal Transplantation

    T. Ishizuka,1 M. Yasuo,1 Y. Ishida,1 H. Miura,1 K. Iwadoh,2 T. Murakami,2 K. Kitajima,2 I. Nakajima,2 S. Fuchinoue.2

    1Transplant Immunology, Tokyo Women's Medical University, Shinjuku ward, Tokyo, Japan; 2Surgery III, Tokyo Women's Medical University, Shinjuku ward, Tokyo, Japan.

    【Background】We experienced a hyperacute rejection (HAR) in ABO-incompatible renal transplantation (ABO-I RTx) on POD 0. We hypothesized that complement-fixing IgG anti-A/B blood group antibody (anti-A/B…
  • 2015 American Transplant Congress

    Long-Term Persistence of Innate Lymphoid Cells in the Gut After Intestinal Transplantation

    J. Weiner, J. Zuber, B. Shonts, S. Yang, M. Martinez, T. Kato, M. Sykes.

    Columbia Center for Translational Immunology, Columbia University Medical Center, New York, NY.

    Introduction: Little is known about innate lymphoid cell (ILC) populations in the human gut, and the turnover of these cells and their subsets after transplantation…
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