Session Time: 2:15pm-3:45pm
Presentation Time: 3:27pm-3:39pm
Location: Room 120-ABC
Histological changes observed on renal allograft biopsies, although considered the current standard for evaluation of allograft injury, lack specificity and disease activity assessment. We devised a protocol for reducing a small fragment of a renal biopsy (3-5mm) to cell suspensions for multicolor flow cytometry and measurement of secreted cytokines. Intra-renal cytokines are measured with Milliplex high sensitivity bead capture assay which uses Luminex technology.
Intra-renal cytokines were then measured in over 200 allograft renal biopsies and normalized to tissue volume. Intra-renal cytokine levels of IL-4, IL-6, IL-8 and IL-10 were compared in transplanted kidneys with normal histology and those with rejection of variable degrees of severity. Rejection and its severity were associated with elevated levels of IL-6, IL-8 and IL-10, and with greater number of CD45+ leukocytes in the biopsy.
|Normal vs Rejection||.81||<.001||<.001||<.001||<.001|
|Mild vs Moderate Rejection||.468||<.001||.011||.007||<.001|
Intra-renal cytokines using only a fragment of a core allograft biopsy can be reliably measured and may provide additional information with potential for refining the current histological assessment.
To cite this abstract in AMA style:Leca N, Anderson S, Muczynski K. Intra-Renal Cytokine Measurement Detected During Kidney Allograft Biopsies Portend Severity of Histological Injury [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/intra-renal-cytokine-measurement-detected-during-kidney-allograft-biopsies-portend-severity-of-histological-injury/. Accessed September 18, 2021.
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