Articles tagged "Endothelial cells"

2018 American Transplant Congress
Mitochondrial Membrane Permeability Plays a Critical Role for Endothelial Cell Necroptosis and Cardiac Allograft Rejection
I. Gan, J. Jiang, D. Lian, X. Huang, W. Liu, A. Haig, A. Jevnikar, Z. Zhang.
Matthew Mailing Centre for Translational Transplant Studies, University of Western Ontario, London, ON, Canada; Multi Organ Transplant Program, London Health Sciences Centre, London, ON, Canada; Pathology and Laboratory Medicine, University of Western Ontario, London, ON, Canada; Medicine, University of Western Ontario, London, ON, Canada.
Background: Transplant injury is invariably associated with programmed cell death resulting in delayed graft function and organ rejection. We were the first to describe receptor-interacting…
2018 American Transplant Congress
Assessing the Impact of Flow in Hypothermic Machine Perfusion on Human Venous Endothelium Using a Novel Ex Vivo Model
Y. Marie,¹ W. Ries,² K. Patel,¹ C. Turnbull,² N. Jamil,⁴ H. Caldwell,³ R. Telfer,² D. Neil,⁵ J. Nath,¹ N. Inston.¹
¹Department of Renal Surgery, Queen Elizabeth Hospital, Birmingham, West Midlands, United Kingdom; ²College of Medical and Dental Sciences, University of Birmingham, Birmingham, West Midlands, United Kingdom; ³Division of Pathology Laboratories, Western General Hospital, Edinburgh, United Kingdom; ⁴Department of Cellular Pathology, Durham and Darlington NHS Foundation Trust, Newcastle, Newcastle Upon Tyne, United Kingdom; ⁵Department of Histopathology, University Hospitals Birmingham NHS Trust, Birmingham, West Midlands, United Kingdom.
BACKGROUNDHypothermic machine perfusion (HMP) involves dynamic flow of chilled preservation fluid through an allograft's vasculature. This study describes a simple, reproducible, human model that allows…
2018 American Transplant Congress
A Novel Endothelial Cell Based Complement Dependent Cytotoxicity Test in Kidney Transplantation
R. Lammerts,¹ J. van den Born,¹ W. Dam,¹ M. Seelen,¹ B. Hepkema,² B. Kroesen,² M. Daha,¹ R. Pol,³ S. Berger,¹ On behalf of the COMBAT Consortium.¹
¹Department of Nephrology, University Medical Centre Groningen, Groningen, Netherlands; ²Department of Immunology, University Medical Centre Groningen, Groningen, Netherlands; ³Department of Surgery, University Medical Centre Groningen, Groningen, Netherlands.
Introduction:Relevant alloantibodies in kidney transplantation comprise anti-HLA antibodies, blood group antibodies (ABO incompatible) and anti-endothelial cell antibodies (AECA) and can initiate antibody-mediated rejection (ABMR). ABMR…
2018 American Transplant Congress
Targeting LTβR Classical NFκB Signaling Facilitates Inflammation Resolution
W. Piao, Y. Xiong, L. Li, V. Saxena, J. Bromberg.
UMB, Baltimore.
Background:Contact hypersensitivity (CHS) is a reliable model to assess the immune cell-mediated cutaneous inflammatory response, which includes the three phases of priming/sensitization, elicitation, and resolution,…
2018 American Transplant Congress
Apoptotic Exosome-Like Vesicles Induce Endothelial Cell Plasticity
F. Migneault,^1,2,4 M. Dieudé,^1,2,4 J. Turgeon,^1,4 D. Beillevaire,^1,2,4 M-.P. Hardy,^2,3,4 C. Perreault,^2,3,4 M-.J. Hébert.^1,2,4
¹CRCHUM, Montreal, QC, Canada; ²Université de Montréal, Montreal, QC, Canada; ³IRIC, Montreal, QC, Canada; ⁴CNTRP, Montreal, QC, Canada.
Acute vascular rejection of renal allografts is associated with increased endothelial apoptosis which contributes to the development of transplant vasculopathy. We previously showed that apoptotic…
2018 American Transplant Congress
Involvement of PD-L1 Upregulation in Anti-A/B Antibody-Induced Graft Accommodation: Reduction of CD4 T Cell Allo-Response against Graft HLA Class II DR
K. Iwasaki,¹ Y. Miwa,¹ H. Hamana,³ H. Kishi,³ A. Muraguchi,³ K. Uchida,¹ T. Kobayashi.²
¹Kidney Disease and Transplant Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; ²Renal Transplant Surgery, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; ³Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
[Background] Antibodies against transplanted organs cause complement-independent graft injury, but there remain many unknown aspects of its mechanism, in particular its effect on allo-response to…
2017 American Transplant Congress
CD4 T Cell Migration Across Lymphatic Endothelium Is Enhanced by Sphingosine 1-Phosphate (S1P) and Differentially Regulated by S1P Receptors (S1PR).
Y. Xiong,¹ C. Brinkman,¹ K. Hippen,² B. Blazar,² J. Bromberg.¹
¹UMB, Baltimore; ²UM, Minneapolis
Introduction: S1PR1 drives T cells to migrate from thymus to blood, and from lymph node (LN) to efferent lymphatics. Whether S1PR1 or other S!PRs regulate…
2017 American Transplant Congress
TIE'ing Statins to Microvascular Stability and Endothelial Cell Immunogenicity.
C. Ghosh,¹ A. Mak,¹ M. McGourty,¹ S. Parikh,² X. Chen,² S. Goldberg,¹ J. Wedel,¹ D. Briscoe.¹
¹Transplant Research Program, Boston Children's Hospital, Boston, MA; ²Division of Nephrology, Beth Israel Deaconess Medical Center, Boston, MA
Graft microvascular endothelial cells (EC) represent a critical interface between the recipient and the donor. Stable/quiescent EC support immunoregulation in part via the expression of…
2017 American Transplant Congress
Lymphotoxin (LT) β Receptor-Mediated Non-Classical NFκB Signaling in Lymphatic Endothelial Eells (LEC) is Required for Homeostatic T Cell Migration.
W. Piao, C. Brinkman, J. Bromberg.
UMB, Baltimore
Background: The selective recruitment of regulatory T cells (Treg) or blockade of memory/effector T cell migration to the graft and draining lymph nodes (dLN) are…
2017 American Transplant Congress
Bioengineering Human Islets to Form Peri-Islet Vessels from Intraislet Endothelial Cells for Improving Human Islet Function.
G. Loganathan, S. Narayanan, M. Dhanasekaran, A. Patel, W. Tucker, M. Hughes, S. Williams, A. Balamurugan.
Clinical Islet Laboratory, Dept. of Surgery, Cardiovascular Innovation Institute, University of Louisville, KY
Native islets in the pancreas have a rich vascular supply however, the current islet isolation technique completely severs the vasculature to islets. As such, these…