Articles tagged "B cells"

2022 American Transplant Congress
Desensitization Using Costimulatory Blockade and Bortezomib to Prevent DSA Formation and Rejection in Reconstructive Transplantation
B. Oh, F. Messner, Y. Guo, G. Brandacher
Johns Hopkins University School of Medicine, Baltimore, MD
*Purpose: Sensitization in form of donor-specific antibodies (DSA) and subsequent antibody-mediated rejection constitute some of the greatest risk factors for allograft rejection and long-term graft…
2022 American Transplant Congress
Antibody Response and Cellular Phenotyping in Kidney Transplant Recipients Following Sars-CoV-2 Vaccination
N. M. Ali¹, J. Miles², S. Mehta¹, V. Tatapudi¹, B. Lonze¹, E. Weldon¹, Z. Stewart¹, C. DiMaggio¹, J. Allen³, S. Gray-Gaillard³, S. Solis³, M. Tuen³, J. Leonard¹, R. Montgomery¹, R. Herati³
¹Transplant Institute, NYU Langone Health, New York, NY, ²CareDx, Brisbane, NY, ³NYU Langone Health, New York, NY
*Purpose: Correlates of protection for SARS-CoV-2 vaccines are not well-established in kidney transplant recipients(KTRs). Studies have highlighted the importance of neutralizing antibodies(Abs), however data suggests…
2022 American Transplant Congress
Marginal Zone Phenotype B Cells Are Required for Formation of Donor-Specific IgG After Allogeneic Sensitization
M. A. Kallarakal, G. Cohen, S. M. Krummey
Department of Pathology, Johns Hopkins University, Baltimore, MD
*Purpose: The formation of allogeneic antibody directed against non-self MHC, termed donor-specific antibody (DSA), is a significant barrier to successful transplant outcomes. Pre-formed DSA limits…
2022 American Transplant Congress
HLA-Specific IgD/CD27 Double Negative B Cell Frequency Assessed by a Novel Multiplex Bead-Based Assay Predicts Rejection in Kidney Transplant Recipients
Z. Ilyas¹, A. Akl², D. Pinelli¹, L. Gallon³, A. Tambur¹, M. Ansari⁴
¹Northwestern University, Chicago, IL, ²Dr. Soliman Fakeeh Hospital, Jeddah, Saudi Arabia, ³Northwestern Univ NMFF, Chicago, IL, ⁴Medicine, Northwestern University, Chicago, IL
*Purpose: Recipients with sensitization to HLA defined by a high percentage of Panel Reactive Antibodies (PRA) or Donor-specific anti-HLA antibodies (DSA) are at higher immunologic…
2022 American Transplant Congress
Perturbing Proteostasis to Deplete Plasma Cells
A. Rossi¹, A. Burg¹, K. Roskin¹, A. Shields², R. Alloway², E. Woodle², D. Hildeman¹
¹Cincinnati Children's Hospital, Cincinnati, OH, ²University of Cincinnati College of Medicine, Cincinnati, OH
*Purpose: Alloantibodies play critical roles in rejection and often prevent sensitized patients from receiving life-sustaining transplants. One approach for enhancing transplantation rates and survival for…
2022 American Transplant Congress
Alloprimed Antibody-Suppressor CD8⁺ TCells Preferentially Kill Alloprimed Germinal Center B Cells
J. Zimmerer, S. Chaudhari, G. Bumgardner
OSUWMC, Columbus, OH
*Purpose: Antibody-mediated rejection (AMR) is a significant contributor to transplant rejection. Our prior work identified a novel subset of CXCR5+CD8+ T cells (CD8+ TAb-supp cells)…
2022 American Transplant Congress
B and Plasma Cell Populations Drive Chronic Rejection After Lung Transplantation
M. Bueno¹, N. F. Smirnova², K. Riemondy³, P. Suresh¹, M. Brown¹, X. Wang¹, S. Collins⁴, J. Sembrat¹, M. Koenigshoff¹, M. Snyder¹, J. McDyer¹, O. Eickelberg¹
¹Pulmonary and Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA, ²I2MC, INSERM UMR1297, Toulouse, France, ³Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, ⁴Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Aurora, CO
*Purpose: Chronic lung allograft dysfunction (CLAD) remains the major determinant of morbidity and mortality post-lung transplantation (LTx). Bronchiolitis obliterans syndrome (BOS) is the most prevalent…
2022 American Transplant Congress
B Cells Fit for Germinal Center Activity Predict Response to a Third Dose of SARS-CoV-2 Vaccine in Solid Organ Transplant Recipients
E. A. Thompson¹, A. H. Karaba¹, K. Cascino¹, K. Roznik¹, T. Chiang¹, L. Biavatti¹, A. T. Abedon¹, J. L. Alejo¹, S. L. Klein¹, J. N. Blankson¹, D. S. Warren¹, C. X. Qin¹, J. Mitchell¹, J. M. Garonzik-Wang², B. J. Boyarsky¹, D. L. Segev¹, A. A. Tobian¹, W. A. Werbel¹, A. L. Cox¹, J. R. Bailey¹
¹Johns Hopkins University, Baltimore, MD, ²University of Wisconsin School of Medicine and Health, Madison, WI
*Purpose: While SARS-CoV-2 vaccination has dramatically reduced COVID-19 severity in the general population, fully vaccinated solid organ transplant recipients (SOTRs) demonstrate reduced seroconversion and increased…
2022 American Transplant Congress
Characterization Of Local Antibody Responses In Human Cardiac Allograft Vasculopathy
S. B. See¹, L. J. Stanton¹, P. Roy¹, S. Mashiko¹, E. R. Vasilescu², C. C. Marboe², M. Szabolcs², M. V. Habal³, M. Farr³, S. Restaino³, Y. Naka⁴, D. M. Mancini⁵, J. C. Madsen⁶, R. G. King⁷, E. P. Kransdorf⁸, E. Zorn¹
¹CCTI, Columbia University Irving Medical Center, New York, NY, ²Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, ³Medicine, Columbia University Irving Medical Center, New York, NY, ⁴Surgery, Columbia University Irving Medical Center, New York, NY, ⁵Cardiology, Icahn School of Medicine, New York, NY, ⁶CTS, Massachusetts General Hospital, Boston, MA, ⁷Microbiology, University of Alabama, Birmingham, AL, ⁸Cedars-Sinai Heart Institute, Los Angeles, CA
*Purpose: Cardiac allograft vasculopathy (CAV) is the leading cause of heart graft recipient mortality after five years post-transplant. A major hurdle to improving outcomes is…
2022 American Transplant Congress
Synergistic Effect of CXCR5⁺CD8⁺ T_Ab-supp Cell Adoptive Cell Therapy and mTOR Inhibition (but Not Calcineurin Inhibition) in Suppressing Alloantibody Following Kidney Transplant in Mice
J. L. Han¹, J. M. Zimmerer¹, Q. Zeng², S. Chaudhari¹, C. K. Breuer², G. L. Bumgardner¹
¹Dept. of Surgery, The Ohio State University, Columbus, OH, ²Center for Regen. Med., The Research Institute at Nationwide Children's Hospital, Columbus, OH
*Purpose: We have reported that CXCR5+CD8+TAb-supp cells mediate suppression of alloantibody (alloAb) production and prolong graft survival in both hepatocellular and kidney transplant (KTx) mouse…