Articles tagged "B cells"

2021 American Transplant Congress
Suppression of T Cell Proliferation by Activated Nonhuman Primate B Cells
K. Lee, K. Deng, G. Huai, F. Qiang, C. Rickert, J. Lei, C. Leguern, J. Markmann
Massachusetts General Hospital, Boston, MA
*Purpose: We previously generated Toll-like receptor (TLR)-activated B cells in rodent settings. These B cells can suppress T cell proliferation via in vitro mixed lymphocytes…
2021 American Transplant Congress
Quantifying Hidden Sensitization: HLA-reactive Memory B Cells in the Spleens of Sensitized Women
J. T. Killian¹, J. A. Houp¹, A. Nellore², R. G. King³, P. M. Porrett¹, F. E. Lund³
¹Department of Surgery, University of Alabama at Birmingham School of Medicine, Birmingham, AL, ²Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, AL, ³Department of Microbiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL
*Purpose: Measurement of circulating anti-HLA antibody (anti-HLA-Ab) in transplant candidates and recipients is required for successful organ transplantation. However, serum Ab measurement alone likely underestimates…
2021 American Transplant Congress
In Vitro Generated Regulatory B Cells Induce Cd4⁺Cd25⁺Foxp3⁺Tregs from Cd4⁺Cd25^–T Cells via TGF-β
G. Huai¹, K. Lee², K. Deng², Q. Fu¹, C. G. Rickert², S. Deng¹, C. LeGuern², J. F. Markmann²
¹Department of Organ Transplantation Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China, ²Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA
*Purpose: Our team previous study showed that TLR-Bregs, stimulated through TLR pathway in vitro, harbored regulatory properties by suppressing CD4+ T cell proliferation and function…
2021 American Transplant Congress
Donor-specific Regulatory B Cells Prolong Skin Graft Survival by Preventing the Proliferation of Donor-specific CD4 Helper but Not of CD8 Effector T Cells
G. Huai¹, K. Lee², K. Deng², Q. Fu¹, C. G. Rickert², S. Deng¹, C. LeGuern², J. F. Markmann²
¹Department of Organ Transplantation Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China, ²Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA
*Purpose: TLR-Bregs were defined by our group as B cells generated in vitro. They have demonstrated the ability to inhibit CD4 T cell proliferation. Since…
2021 American Transplant Congress
Properties of Regulatory B Cells Suppressing B Cells
Q. Fu¹, K. Lee², K. Deng², G. Huai², C. Rickert², H. Yang¹, C. LeGuern², S. Deng¹, J. F. Markmann²
¹Organ Transplantation Center, Sichuan Provincial People's Hospital, University of Electronic Sciences and Technology of China, Chengdu, China, ²Center for Transplantation Sciences, MGH, Boston, MA
*Purpose: Our previous study showed that TLR-Bregs, regulatory B cells generated in vitro through TLR activation, suppressed naïve B cell proliferation triggered by a mitogen…
2021 American Transplant Congress
Lung Transplant Recipients Developing Early DSAs are Characterized by a Shift Towards Memory B Cells Directly After Transplantation
S. Christoph¹, A. Hitz¹, R. Bellmàs Sanz¹, J. Kühne¹, B. Wiegmann², F. Ius², J. Salman², E. Chichelnitskiy¹, T. Siemeni², W. Sommer³, A. Haverich², G. Warnecke³, C. Falk¹
¹Institute of Transplant Immunology, MHH, Hannover, Germany, ²Department of Cardiothoracic, Transplant and Vascular Surgery, MHH, Hannover, Germany, ³Department of Cardiac Surgery, University Hospital Heidelberg, Heidelberg, Germany
*Purpose: After lung transplantation (LTx), the development of early donor HLA-specific antibodies (eDSA) is associated with antibody-mediated rejection (AMR) and poor graft survival. Since 2013,…
2021 American Transplant Congress
Belatacept and Rapamycin Maintenance Immunosuppression in a Sensitized Nonhuman Primate Kidney Allotransplantation Model
R. Schmitz¹, Z. W. Fitch¹, M. Manook¹, J. Yoon¹, A. B. Farris², J. Kwun¹, S. J. Knechtle¹
¹Department of Surgery, Duke University Medical Center, Durham, NC, ²Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA
*Purpose: Desensitization with costimulation blockade and proteasome inhibition prior to kidney transplantation prevents early antibody-mediated rejection (AMR) and prolongs graft survival in highly sensitized nonhuman…
2021 American Transplant Congress
Single Cell RNA Sequencing of Tim1- B Cells and Tim1+ Regulatory B Cells
Q. Fu¹, K. Lee², K. Deng², D. Agarwal³, P. v. Galen⁴, C. G. Rickert², G. Huai², H. Yang¹, C. LeGuern², S. Deng¹, J. F. Markmann²
¹Organ Transplantation Center, Sichuan Provincial People's Hospital, University of Electronic Sciences and Technology of China, Chengdu, China, ²Center for Transplantation Sciences, MGH, Boston, MA, ³Division of Transplantation, Department of Surgery, Hospital of the University of Pennsylvania, Philadelphia, PA, ⁴Division of Hematology, Brigham and Women's Hospital, Boston, MA
*Purpose: Tim1 signaling has an important role in inducing and maintaining the function of Regulatory B cells. Our previous study showed that Tim1+ Bregs prolong…
2020 American Transplant Congress
Next Generation Sequencing B Cell Receptor Reveals B Cell Clones Encoding HLA Antibody in Transplant Recipients
X. Zhang
HLA and Immunogenetcis Laboratory /CTC, Cedars Sinai Medical Center, Los Angeles, CA
*Purpose: The development of HLA donor specific antibodies (DSA) increases the risk of antibody mediated rejection and is an independent predictor of poor patient survival.…
2020 American Transplant Congress
Effect of Rituximab Dose as Induction Therapy in ABO Incompatible Living Kidney Transplantation A Systemic Review and Network Meta Analysis
S. Hwang¹, W. Park², K. Kim¹, S. Lee¹, J. Song¹
¹Inha University, Incheon, Korea, Republic of, ²Keimyung University, Daegu, Korea, Republic of
*Purpose: ABO-incompatible living kidney transplantation (ABOILKT) has steadily become more widespread. However, the optimal immunosuppressive regimen for ABOILKT remains uncertain. We aimed to determine the…