Articles tagged "Adhesion molecules"

2019 American Transplant Congress
Proteomics Reveals Compartment-Specific Alterations in the Extracellular Matrix of Kidney Allografts with Antibody-Mediated Rejection
S. Clotet Freixas¹, C. McEvoy¹, I. Batruch², M. Kotlyar³, C. Pastrello³, J. Van¹, A. Bozovic¹, V. Kulasingam¹, P. Chen⁴, I. Jurisica³, A. Chruscinski¹, R. John¹, A. Konvalinka¹
¹University Health Network, Toronto, ON, Canada, ²Lunenfeld-Tanenbaum Research Institute, Toronto, ON, Canada, ³Krembil Research Institute, Toronto, ON, Canada, ⁴Institute of Medical Science, University of Toronto, Toronto, ON, Canada
*Purpose: Antibody-mediated rejection (AMR) is the most important cause of premature kidney graft loss. AMR is caused by anti-HLA and non-HLA antibodies against proteins in…
2019 American Transplant Congress
Inhibition of Sialidase Activity and Galectin Binding Reduces Xenogeneic Neutrophil-Endothelial Adhesion
B. M. Cerel¹, B. M. French², D. G. Harris², C. Laird², L. Burdorf¹, X. Cheng², C. Feng³, A. S. Cross⁴, S. E. Goldblum⁴, G. R. Vasta³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Deparment of Surgery, Massachusetts General Hospital, Boston, MA, ²Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, ³Department of Microbiology and Immunology, and Institute of Marine and Environmental Technology, University of Maryland School of Medicine, Baltimore, MD, ⁴Department of Medicine Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD
*Purpose: Immediate neutrophil sequestration occurs despite genetic modifications (Gal-a-1-3 galactosyl transferase knock out; human CD46 complement regulatory gene addition) to pig donor organs. Sialic acids…
2019 American Transplant Congress
Ligation Of Hla Class Il Molecules Disrupts Endothelial Barrier Through Src-dependent Ve-cadherin Destabilization
F. Li¹, R. A. Sosa¹, N. M. Valenzuela¹, E. Rozengurt², E. F. Reed¹
¹Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA, ²Digestive Diseases Research Center, University of California, Los Angeles, Los Angeles, CA
*Purpose: Microvascular inflammation, vascular permeability, and accumulation of intravascular mononuclear cells are characteristics of acute and chronic antibody-mediated rejection. Vascular endothelial cadherin (VE-Cadherin) is a…
2019 American Transplant Congress
Sphingosine 1-Phosphate Receptor 2 Regulates Migration through Endothelial Cell VCAM and Adherens Junctions
Y. Xiong¹, W. Piao¹, C. Brinkman¹, L. Li¹, J. Kulinski², A. Olivera², A. Cartier³, T. Hla³, K. Hippen⁴, B. Blazar⁴, S. Schwab⁵
¹Surgery, UMB, Baltimore, MD, ²NIH, Bethesda, MD, ³Boston Children's hospital, Boston, MA, ⁴U.Minnesota, Minneapolis, MN, ⁵NYU, NY, NY
*Purpose: Lymphatic endothelial cells (LEC) highly expres S1PR1 and S1PR2. The ligand S1P is produced by LEC and regulates T cell migration. The mechanisms of…
2018 American Transplant Congress
Endothelial Cell Heterogeneity Governs Basal and Inducible Inflammatory Responses Relevant to Allograft Injury
N. Valenzuela.
Pathology and Laboratory Medicine, UCLA, Los Angeles, CA.
PurposeTransplanted tissues are differentially susceptible to ischemia/reperfusion and rejection. Endothelial cells (EC) are active regulators of inflammation. But model EC may not adequately reflect the…
2018 American Transplant Congress
CD4 T Cells Require Both S1PR1 and S1PR4 for Afferent Lymphatic Migration
Y. Xiong,¹ W. Piao,¹ C. Brinkman,¹ L. Li,¹ J. Kulinski,² A. Olivera,² A. Cartier,³ T. Hla,³ S. Schwab,⁴ K. Hippen,⁵ B. Blazar,⁵ J. Bromberg.¹
¹UMB, MD; ²NIH, MD; ³Boston Children's Hospital, MA; ⁴NYU, NY; ⁵U. Minnesota, MN.
Introduction: Sphingosine 1-phospate (S1P) receptor 1 (S1PR1) drives T cell migration from thymus into blood, and lymph nodes (LN) into efferent lymphatics. Whether S1PR1 and…
2018 American Transplant Congress
Lymphatic Endothelium Requires S1PR2 for T Cell Transendothelial Migration
Y. Xiong,¹ W. Piao,¹ C. Brinkman,¹ L. Li,¹ J. Kulinski,² A. Olivera,² A. Cartier,³ T. Hla,³ S. Schwab,⁴ K. Hippen,⁵ B. Blazar,⁵ J. Bromberg.¹
¹UMB, MD; ²NIH, MD; ³Boston Children's Hospital, MA; ⁴NYU, NY; ⁵U. Minnesota, MN.
Introduction: Sphingosine 1-phosphate (S1P) receptors 1 (S1PR1) and S1PR2 are highly expressed by lymphatic endothelial cells (LEC), while T cells express S1PR1 and S1PR4. S1P…
2018 American Transplant Congress
Involvement of PD-L1 Upregulation in Anti-A/B Antibody-Induced Graft Accommodation: Reduction of CD4 T Cell Allo-Response against Graft HLA Class II DR
K. Iwasaki,¹ Y. Miwa,¹ H. Hamana,³ H. Kishi,³ A. Muraguchi,³ K. Uchida,¹ T. Kobayashi.²
¹Kidney Disease and Transplant Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; ²Renal Transplant Surgery, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; ³Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
[Background] Antibodies against transplanted organs cause complement-independent graft injury, but there remain many unknown aspects of its mechanism, in particular its effect on allo-response to…
2018 American Transplant Congress
Treg Regulate Distinct Lymphatic NFκB Signaling Pathways to Alter Migration and Immunity
W. Piao, Y. Xiong, L. Li, V. Saxena, J. Bromberg.
UMB, MD.
Background: Treg migration to the graft and draining lymph nodes is crucial for graft survival. Little is known about the molecular cues that regulate the…
2018 American Transplant Congress
Increased Expression of CEACAM1 is Cytoprotective during Ischemia-Reperfusion Injury in Human Orthotopic Liver Transplantation
R. Sosa,¹ J. Nevarez-Mejia,¹ M. Rossetti,¹ A. Zarrinpar,² F. Kaldas,² C. Lassman,¹ B. Naini,¹ N. Datta,² R. Busuttil,² D. Gjertson,¹ J. Kupiec-Weglinski,² E. Reed.¹
¹Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA; ²Surgery, University of California, Los Angeles, Los Angeles, CA.
Background T cell exhaustion has recently been proposed as an alternative mechanism to prevent memory development and tissue damage arising during ischemia-reperfusion injury (IRI) in…