Validation Study of Operational Tolerance Signatures in Korean Kidney Transplantation Recipients
1Department of Nephrology, School of Medicine, Kyung Hee University, Seoul, Korea
2Department of Nephrology, Kyung-pook National University School of Medicine, Daegu, Korea
3Department of Nephrology, Sungkyunkwan University, Samsung Hospital, College of Medicine, Seoul, Korea
4Department of Nephrology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Meeting: 2018 American Transplant Congress
Abstract number: A15
Keywords: Graft survival, Kidney transplantation, Tolerance
Session Information
Session Name: Poster Session A: Biomarkers, Immune Monitoring and Outcomes
Session Type: Poster Session
Date: Saturday, June 2, 2018
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall 4EF
Background:A peripheral B cell population was significantly higher in drug-free operationally tolerant patients than those with stable graft function or acute rejection under standard immunosuppression. Molecular signatures related to B cell have recently been reported that the expression levels were significantly higher in operationally tolerant patients or functionally stable graft patients receiving long-term immunosuppression compared to rejection group. Therefore, we investigated whether seven genes reported in previous studies are validated in Korean kidney transplant patients and can be useful to monitor immune quiescence in patients who have long-term graft survival (LGS) with stable kidney function (eGFR [sup3] 50 ml/min/1.73m2) over 10 years.
Methods: A total of153 samples (30 stable (STA), 63 acute rejection (AR), 38 long-term graft survival (LGS), 15 chronic antibody-mediated rejection (cAMR) and 7 operational tolerance (TOL)) were collected after kidney transplantation. RNA and PBMCs were obtained from peripheral bloods and analyzed by real-time PCR and flow cytometry.
Results: The expression levels of BNC2, IGKV1D-13, and IGKV4-1 among seven tolerance signatures were significantly increased in peripheral blood of TOL patients compared to those with stable graft function and patients with acute rejection. These three genes were also significantly increased in LGS group compared to patients with STA or AR. The correlation of IGKV1D-13 and IGKV4-1 related to B cell were better than the correlation of B-cell signatures and BNC2. In addition, immature B cell (CD24+CD38+/CD19+ B cell) population in peripheral blood was significantly elevated in TOL patients. Finally, three genes signatures effectively discriminated TOL patients from cAMR group.
Conclusion: We validated an association of B-cell signatures with controlled alloimmune response in Korean kidney transplant recipients.
CITATION INFORMATION: Seo J., Lee Y., Kim Y., Moon J., Jeong K-.H., Kim C-.D., Park J., Jeong B., Lee S-.H. Validation Study of Operational Tolerance Signatures in Korean Kidney Transplantation Recipients Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:
Seo J, Lee Y, Kim Y, Moon J, Jeong K-H, Kim C-D, Park J, Jeong B, Lee S-H. Validation Study of Operational Tolerance Signatures in Korean Kidney Transplantation Recipients [abstract]. https://atcmeetingabstracts.com/abstract/validation-study-of-operational-tolerance-signatures-in-korean-kidney-transplantation-recipients/. Accessed May 16, 2025.« Back to 2018 American Transplant Congress