Date: Saturday, June 11, 2016
Session Name: Poster Session A: Kidney: Acute Cellular Rejection
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Halls C&D
The discovery of sensitive noninvasive diagnosis and the development of immune management in the renal allograft recipients are required for long-term graft survival. Although the several studies have reported numerous genetic biomarkers, the targets were not sufficiently useful to detect clinically renal graft injury. In this study, we performed DNA microarray and our data provide candidate genes for diagnosing acute rejection, chronic allograft dysfunction, and tolerance in peripheral blood.
110 blood samples were collected from 108 transplant recipients at five centers (24 patient with long-term survival without biopsy, 6 patients with tolerance, 25, 14, 14, 6, and 21 patients with biopsy-proven ACR, AMR, chronic AMR, other graft injury (OGI), and stable graft function (STA), respectively). Gene expression was profiled using DNA microarray of Agilent SurePrint G3 Human GE (60K), and significantly differential expressed genes (DEGs) were screened between clinical subtypes by SAM analysis. KEGG pathway was used to obtain pathways.
In microarray results, 55 transcripts were differentially expressed in recipients with ACR, and 3933 were differentially expressed in AMR compared with stable group. 3472 and 2071 transcripts were differentially expressed in long-term survival or tolerance group, respectively compared with STA by SAM analysis. DEGs in ACR were related to ribosome and hematopoietic cell lineage pathway, and those in AMR were related to systemic lupus erythematosus pathway. Oxidative phosphorylation, spliceosome, epithelial cell signaling in H, RNA polymerase and cytosolic DNA-sensing pathway in LTS group and natural killer cell mediated c and prion diseases pathway in tolerance group were classified.
We investigated genetic biomarkers to develop a noninvasive assay in peripheral blood to discriminate patients with AR from stable graft function as well as LTS and tolerance by transcriptome analysis. The differentially expressed genes were more identified in AMR, LTS and tolerance compared with STA group than in ACR group. Further study to validate the target genes in independent samples is requested for clinical application. If validated the genes as a noninvasive assay may be useful to diagnose acute rejection and help to effectively treat immunosuppressive drugs for long-term graft survival.
CITATION INFORMATION: Seo J.-W, Moon H, Kim Y.-G, Jeong K.-H, Ihm S.-J, Moon J.-Y, Lee S.-H. Transcriptome Profiles in Peripheral Blood of Kidney Graft Recipients. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:Seo J-W, Moon H, Kim Y-G, Jeong K-H, Ihm S-J, Moon J-Y, Lee S-H. Transcriptome Profiles in Peripheral Blood of Kidney Graft Recipients. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/transcriptome-profiles-in-peripheral-blood-of-kidney-graft-recipients/. Accessed September 22, 2019.
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