ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

The Mark of Zorro: IL35-Coated Exosomes Cross-Dress and Exhaust Recipient T & B Cells during Transplant Tolerance

W. Burlingham,1 J. Sullivan,1 D. Lema,1 Y. Tomita,2 Y. Zhou,1 D. Vignali.3

1Surgery, University of Wisconsin, Madison, WI
2Surgery, Tokyo Women's Hospital, Tokyo, Japan
3Immunology, University of Pittsburgh Medical Center, Pittsburgh, PA.

Meeting: 2018 American Transplant Congress

Abstract number: 264

Keywords: CD4, Mice, T cells, Tolerance

Session Information

Session Name: Concurrent Session: Novel Regulatory Pathways in Tolerance

Session Type: Concurrent Session

Date: Monday, June 4, 2018

Session Time: 2:30pm-4:00pm

 Presentation Time: 3:18pm-3:30pm

Location: Room 606/607

The folk-hero "Zorro" left his mark on by a sword-slashed "Z". In transplant recipients, rare Tregs also leave a mark on their conventional T & B LC targets. This mark is caused by Ag-specific CD4 Tregs that release IL35-coated exosomes, “cross-dressing” (xD) their targets with IL35. Hypotheses: 1) IL35 suppression by passenger Tregs is greatly amplified by xD of host T cells; 2) monitoring CD4+ T cells displaying IL35/xD-phenotype after heterotopic heart transplantation will correlate with biD regulation(HvG and GvH), and 3) that IL-35-induced “exhaustion” is a direct result of the IL35/xD-phenotype. Approach: We tolerized B6 “donor” mice with CBA DST + 125 ug (x3) MR1(anti-CD40L). We sub-optimally tolerized recipient CBA mice with B6 DST + 62.5 ug(x3) injections of MR1. On d.35, the CBA mice received either a normal B6 heart allograft(uni-D regulation) or one from a CBA-tolerized B6 donor(biD regulation).Results: While the uni-D CBA showed prolonged allograft survival, by 4 weeks all grafts were rejected. When the heart from a tolerized B6 mouse was transplanted in the same recipient, all grafts survived long term. There was an initial slight increase in % surface Ebi3+ T-CD4 cells in the uni-D mice, then the % began to drop at 2 wks and was even lower at the time of graft rejection (4 wk). In contrast, the PBMC-CD4 T cell % of IL35-xD cells continued to climb at 2 & 4 wks in the biD group, after which the level stabilized, suggesting an amplified impact of IL35 exosome production from the donor side. Population microscopy, depicting IL35-xD cells showed punctate surface staining of Ebi3 that was similar in CD4 T, CD8 T, and B cell subsets. A similar, but non-identical, pattern of p35 expression was seen in Ebi3- xD cells. Exhaustion was confined to the IL35-xD cells and was absent from non-IL35 xD cells. Conclusion: Tregs produce IL35-coated exosomes. These exosomes left a “mark”, punctate surface IL35 expression, while also promoting exhaustion in T and B cells.

CITATION INFORMATION: Burlingham W., Sullivan J., Lema D., Tomita Y., Zhou Y., Vignali D. The Mark of Zorro: IL35-Coated Exosomes Cross-Dress and Exhaust Recipient T & B Cells during Transplant Tolerance Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Burlingham W, Sullivan J, Lema D, Tomita Y, Zhou Y, Vignali D. The Mark of Zorro: IL35-Coated Exosomes Cross-Dress and Exhaust Recipient T & B Cells during Transplant Tolerance [abstract]. https://atcmeetingabstracts.com/abstract/the-mark-of-zorro-il35-coated-exosomes-cross-dress-and-exhaust-recipient-t-b-cells-during-transplant-tolerance/. Accessed May 21, 2025.

« Back to 2018 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences