Date: Tuesday, May 5, 2015
Session Name: Poster Session D: Innate Immunity in Transplantation
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
AIM- Infectious complication is the leading cause of graft and patient loss after renal transplantation (RT). Under immunosuppressive condition when acquired immunity is suppressed, components of innate immunity like FcγRIIA, FcγRIIIA and C1QA plays a great role in preventing infectious complication after RT. Therefore, our present study investigated the role of gene single nucleotide polymorphism (SNP) of those components of innate immunity on infectious complication and survival after RT.
Method: 71 consecutive RT patients were enrolled in our study. The SNPs of FcγRIIA (131 H/R), FcγRIIIA(158 F/V) and C1QA (276 A/G) were defined by performing PCR-RFLP (PCR-Restricted Fragment Length Polymorphism) method following extraction of genomic DNA from peripheral blood mononuclear cell of the recipients. Those SNPs were analyzed with respect to clinical outcome of infectious complication within 3 months of transplantation i.e. incidence of cytomegalovirus (CMV) infection and blood stream infection (BSI).
Results: We observed that incidence of BSI were statistically significant (p=0.018) in the patients with F carrier of FcγRIIIA (n=5/33, 15.15%) than to VV homozygous (n=0/38, 0%). Similarly, we also observed that incidence of CMV infection tends to be higher (p=0.084) in the patients with AA homozygous of C1QA (n=10/16, 62.5%) than in G carrier (n=23/55, 41.81%). FcγRIIA has no influence on any of the infectious complications. We did not find any association with graft survival with respect to those 3 gene SNPs.
Conclusion: The SNP in FcγRIIIA gene in RT recipients was greatly associated with the susceptibility or resistance to blood stream infection, i.e. F carriers may be individuals with high risk of BSI. C1QA gene polymorphism also tends to effect incidence of CMV infection after RT, i.e. AA homozygous individuals seems to have high risk of CMV infection. These SNPs may help to decide the intensive prophylactic therapy with antibiotics or antiviral therapy on the suspected individuals.
To cite this abstract in AMA style:Das L, Tanaka Y, Verma S, Shimizu S, Ide K, Ohdan H. The Impact of FcγRIIA, FcγRIIIA and C1QA Genes Polymorphism On Infectious Complications Following Renal Transplantation [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/the-impact-of-fcriia-fcriiia-and-c1qa-genes-polymorphism-on-infectious-complications-following-renal-transplantation/. Accessed October 31, 2020.
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