The Effects of Brain Death and Ischemia on Tolerance Induction Are Organ-Specific.
1Center for Transplantation Sciences, Massachusetts General Hospital and Harvard Medical School, Boston, MA
2Department of Cardiac Surgery, Ludwig-Maximilians-University, Munich, Germany
3Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA
4Division of Thoracic Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA
5Division of Cardiac Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA.
Meeting: 2016 American Transplant Congress
Abstract number: C132
Keywords: Brain death, FK506, Ischemia, Tolerance
Session Information
Session Name: Poster Session C: Ischemia Reperfusion Injury and Organ Preservation
Session Type: Poster Session
Date: Monday, June 13, 2016
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Halls C&D
Background. We have previously shown that 12 days of high-dose calcineurin inhibition induced tolerance in miniature swine transplanted with MHC mismatched lung or cotransplanted heart and kidney allografts. However, if the lung grafts were procured from brain dead donors, and transplanted alone, they rejected within 19-45 days. Here, we investigated whether donor brain death and/or allograft ischemia would also prevent tolerance induction in heart plus kidney recipients.
Methods. Heart and kidney cotransplantation was performed with a 12-day course of tacrolimus in 8 miniature swine. Organs were retrieved from donors rendered brain dead for 4 hours (n=2), 8 hours (n=2) or 4 hours with 4 additional hours of cold storage (n=2). In two additional recipients of non-brain dead organs, prolonged warm ischemia of the kidney graft was induced by renal artery clamping.
Results. In contrast to lung allograft recipients, all heart and kidney recipients that completed their protocols achieved long term tolerance (allografts survival of greater than 100 days with no in vitro or in vivo evidence of rejection) despite brain death and ischemia.
Conclusion. These data suggest that the deleterious effects of brain death and ischemia on tolerance induction are organ-specific.
CITATION INFORMATION: Michel S, Madariaga M, LaMuraglia II G, Colvin R, Farkash E, Allan J, Sachs D, Yamada K, Madsen J. The Effects of Brain Death and Ischemia on Tolerance Induction Are Organ-Specific. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:
Michel S, Madariaga M, II GLaMuraglia, Colvin R, Farkash E, Allan J, Sachs D, Yamada K, Madsen J. The Effects of Brain Death and Ischemia on Tolerance Induction Are Organ-Specific. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/the-effects-of-brain-death-and-ischemia-on-tolerance-induction-are-organ-specific/. Accessed May 11, 2025.« Back to 2016 American Transplant Congress