ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

T Cell Immunomonitoring Including Donor Specific Treg Expansion Prospectively Predicts Renal Allograft Tolerance Induced by Transient Mixed Chimerism

K. Hotta, T. Oura, A. Dehnadi, K. Huh, M. Matsunami, H. Sasaki, G. Benichou, J. Madsen, A. Cosimi, T. Kawai.

Center for Transplant Science, Massachusetts General Hospital, Boston, MA.

Meeting: 2018 American Transplant Congress

Abstract number: A414

Keywords: Kidney transplantation, Monitoring, T cells, Tolerance

Session Information

Date: Saturday, June 2, 2018

Session Name: Poster Session A: Tolerance / Immune Deviation

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Hall 4EF

Related Abstracts
  • Donor Specific Expansion of Regulatory T-Cells Was Associated With Allograft Tolerance Induced By Transient Mixed Chimerism in Nonhuman Primates
  • Immune Tolerance Monitoring in Renal Allograft Tolerance Induced by Transient Mixed Chimerism in Nonhuman Primates.

Background: Successful induction of allograft tolerance in MHC-mismatched kidney transplantation has been achieved after induction of transient chimerism in both nonhuman primates (NHP) and humans. However, some recipients developed acute rejection (AR) despite of development of chimerism. Therefore, development of a reliable assay to monitor tolerance is critically important to safely taper immunosuppression. In this study, we prospectively studied whether T cell immunomonitoring is useful to predict transplant outcome in recipients of combined kidney and donor bone marrow transplantation (DBMT).

Method: Eleven NHP recipients who received DBMT either simultaneously or 2-4 months after kidney transplantation were studied. These recipients received a nonmyeloablative conditioning regimen that included low dose TBI and/or thymic irradiation, costimulatory blockade, ATG and a one month course of CyA. Five monkeys received a Bcl-2 inhibiter. We monitored T cell responses against donor and 3rd party cells using CFSE-MLR co-stained for CD4, CD8 Foxp3, INF-γ and IL-4.

Results: All recipients developed transient mixed chimerism up to 2 months after DBMT. We performed CFSE-MLR assay at 114±40 day after transplantation when these recipients had normal kidney function. Seven recipients later developed AR and four achieved long-term renal allograft survival without immunosuppression (>300 days) (LTS). Although CD8+ T cell hypo-responsiveness was observed in LTS recipients, the exhibited substantial anti-donor CD4+ T cell proliferation comparable to that observed in AR recipients. However, among these proliferated CD4+ cells, Foxp3+ Tregs were significantly higher in LTS recipients comparing with AR recipients (P<0.05). In ROC curve analysis, AUC for percentage of proliferating Treg is 0.92 (P<0.05). On the other hand, INF-γ+ and IL-4 CD4+ cell proliferation was similar between two groups.

Conclusion: Immunomonitoring of donor specific Treg expansion may reliably predict renal allograft tolerance.

CITATION INFORMATION: Hotta K., Oura T., Dehnadi A., Huh K., Matsunami M., Sasaki H., Benichou G., Madsen J., Cosimi A., Kawai T. T Cell Immunomonitoring Including Donor Specific Treg Expansion Prospectively Predicts Renal Allograft Tolerance Induced by Transient Mixed Chimerism Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Hotta K, Oura T, Dehnadi A, Huh K, Matsunami M, Sasaki H, Benichou G, Madsen J, Cosimi A, Kawai T. T Cell Immunomonitoring Including Donor Specific Treg Expansion Prospectively Predicts Renal Allograft Tolerance Induced by Transient Mixed Chimerism [abstract]. https://atcmeetingabstracts.com/abstract/t-cell-immunomonitoring-including-donor-specific-treg-expansion-prospectively-predicts-renal-allograft-tolerance-induced-by-transient-mixed-chimerism/. Accessed March 1, 2021.

« Back to 2018 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Enhanced Recovery after Surgery Protocols in Kidney Transplantation: Time to Change Practice?
  • Efficacy of Filgrastim in Solid Organ Transplant Recipients
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • #16789 (not found)
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.