Date: Sunday, April 30, 2017
Session Name: Poster Session B: Allorecognition and T Cell Biology
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall D1
Ageing is associated with changes in the peripheral T-cell immune system, which can be significantly influenced by latent cytomegalovirus (CMV)-infection. Therefore, we wanted to investigate how changes in circulating T-cell populations correlate with T-cell composition of the lymph node (LN), which is crucial for a comprehensive understanding of the T-cell system.
Materials and methods
T cells from peripheral blood (PB) and LN of 38 end-stage renal disease patients were analyzed for frequency of recent thymic emigrants (using CD31 expression within naive cells and T-cell receptor excision circle (TREC) content), relative telomere length and expression of differentiation markers.
Compared with PB, LN contained relatively more CD4+ than CD8+ T cells (p<0.001). The TREC-content in T cells was significantly higher in the LN (p=0.002). The percentage of naive and central memory (CM) CD4+ and CD8+ T cells and thymic output parameters, showed a strong linear correlation between PB and LN (p=0.026 for CM CD4+ T cells and p<0.001 for the other parameters). Highly differentiated CD28null T cells, being CD27–, CD57+ or PD-1+ were almost exclusively found in the circulation but not in LN. An age-related decline in naive CD4+ and CD8+ T-cell frequency was observed (p=0.035 and p=0.002, respectively) within LN, concomitant with an increase in central memory CD8+ T cells (p=0.033). Latent CMV-infection dramatically increased the frequency of circulating terminally differentiated T cells, leading to increased frequencies of CD4+CD28null and CD8+CD28null T cells showing decreased expression of CD27 and increased expression of PD-1 and CD57. However, this effect of CMV was not observed in the LN-derived T-cell population.
Overall T-cell composition and measures of thymic function in PB and LN are strongly correlated. However, highly differentiated CD28null T cells, which may comprise a large part of circulating T cells in CMV-seropositive individuals, are almost exclusively found within the circulation.
CITATION INFORMATION: Dedeoglu B, de Weerd A, Huang L, Langerak A, Klepper M, Verschoor W, Reijerkerk D, Baan C, Litjens N, Betjes M. T-Cell Aging Parameters in the Lymph Node and Peripheral Blood Are Strongly Associated but Terminally Differentiated T Cells Are Mainly Located within the Circulation. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Dedeoglu B, Weerd Ade, Huang L, Langerak A, Klepper M, Verschoor W, Reijerkerk D, Baan C, Litjens N, Betjes M. T-Cell Aging Parameters in the Lymph Node and Peripheral Blood Are Strongly Associated but Terminally Differentiated T Cells Are Mainly Located within the Circulation. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/t-cell-aging-parameters-in-the-lymph-node-and-peripheral-blood-are-strongly-associated-but-terminally-differentiated-t-cells-are-mainly-located-within-the-circulation/. Accessed May 25, 2019.
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