Date: Tuesday, May 5, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Introduction: While a diffuse positivity (>50%) of C4d in kidney transplant peritubular capillaries strongly co-relates with the presence of acute or chronic antibody mediated rejection, definitive data on the significance of focal (10-50%) C4d deposits on graft outcomes are still lacking. We present a single center experience of C4d staining in kidney transplant biopsies.
Methods: All consecutive indication-driven (increase in serum creatinine +/- proteinuria) biopsies in primary kidney transplant recipients done at our institution between December 2011 and March 2013 were retrospectively reviewed. C4d positivity was assessed by immunohistochemistry along with conventional histopathological examination. Biopsies were classified as C4d negative (<10%), with focal (10%-50%) or with diffuse (>50%) C4d deposits. Antibody Mediated Rejection (AMR) and Cell Mediated Rejection (CMR) were diagnosed using Banff 2007 criteria.
Results: Patient characteristics are summarized in Table 1.
|Mean Age (y)||53 +/- 15.4|
|Race||66% African Americans|
|Mean cPRA (%)||16.9 +/- 28.3|
|Donor type||73% Deceased Donors|
|Mean time after transplantation to biopsy (d)||747 +/- 1093|
Conclusion: Our data suggest no implication of focal C4d positivity in peritubular capillaries in kidney transplant biopsies in diagnosis of AMR, CMR or incidence of graft loss. We are currently evaluating association of Donor Specific Antibodies with focal C4d positivity in kidney transplant biopsies and its impact on graft survival.
To cite this abstract in AMA style:Prashar R, Yessayan L, Goggins M, Karthikeyan V, Venkat K, Patel A. Single Center Experience of C4d Staining of Kidney Transplant Biopsies [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/single-center-experience-of-c4d-staining-of-kidney-transplant-biopsies/. Accessed February 24, 2020.
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