Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall 4EF
Background: Reduced immunosuppression in a key therapy for BK virus (BKV)-associated nephropathy (BKVAN) in kidney transplant (KT) recipients. A study of immune monitoring against BKV is scarce. We investigated BKV-specific T cell immunity in KT recipients at our institution.
Methods: All adult KT recipients with BKVAN (January to November2017) were included. Laboratory-developed intracellular cytokine assay measuring %IFN-γ producing CD4+&CD8+ Tcells, using large-T antigen (LT) & viral capsid protein 1 (VP1), at diagnosis & 3 months after reduction of immunosuppression were assessed.
Results: We included 12 KT recipients with BKVAN [(proven (n=7), presumptive (n=4), possible (n=1)]. Those with presumptive BKVAN had a median plasma BKV load of 5.9log10 cps/mL (IQR 4.9-6.1). Mycophenolate mofetil was discontinued in all patients. Four(33%) patients achieved BKV clearance & 8(67%) patients remained with persistent BKV DNAemia. There was a significant increase in mean of %IFN-γ producing CD4+T cells to LT after reduction of immunosuppression compared to at diagnosis [0.004vs.0.015 (p= 0.047)]. A trend of increasing in mean of %IFN-γ producing CD4+ T cells to VP1 and CD8+ T cells to LT&VP1 was also observed (P= NS).
|Mean IFN-γ producing T-cells (%)||P value|
|At diagnosis||After reduction of immunosuppression|
Conclusions: Our pilot study showed a trend of increased BKV-specific T cell immunity after reduction of immunosuppression. A larger study is needed to assess specific immunity as a potential tool to manage BKVAN.
CITATION INFORMATION: Bruminhent J., Srisala S., Klinmalai C., Pinsai S., Watcharananan S., Kantachuvessiri S., Hongeng S., Apiwattanakul N. Role of BK Virus-Specific T Cell Immune Monitoring for BK Virus-Associated Nephropathy in Kidney Transplant Recipients Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Bruminhent J, Srisala S, Klinmalai C, Pinsai S, Watcharananan S, Kantachuvessiri S, Hongeng S, Apiwattanakul N. Role of BK Virus-Specific T Cell Immune Monitoring for BK Virus-Associated Nephropathy in Kidney Transplant Recipients [abstract]. https://atcmeetingabstracts.com/abstract/role-of-bk-virus-specific-t-cell-immune-monitoring-for-bk-virus-associated-nephropathy-in-kidney-transplant-recipients/. Accessed December 7, 2019.
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