ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Reverse Signaling through CD137 Ligand Promotes Tissue Repair after Ischemia-Reperfusion Kidney Injury

H. R. Cho1, S. Lee2, J. S. Lee3, S. J. Park1, B. Kwon2

1Ulsan University Hospital, Ulsan, Korea, Republic of, 2Biomedical Research Center, Ulsan University Hospital, Ulsan, Korea, Republic of, 3Department of Internal Medicine, Ulsan University Hospital, Ulsan, Korea, Republic of

Meeting: 2019 American Transplant Congress

Abstract number: A93

Keywords: Renal injury, Renal ischemia

Session Information

Session Name: Poster Session A: Ischemia Reperfusion & Organ Rehabilition

Session Type: Poster Session

Date: Saturday, June 1, 2019

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Hall C & D

*Purpose: We previously showed that CD137 ligand (CD137L) reverse signaling play a central role in kidney ischemia-reperfusion injury (IRI) by amplifying acute renal injury. In this study, we explored whether CD137L signaling is involved in the tissue repair process during kidney IRI.

*Methods: Kidney ischemia-reperfusion injury was induced in C57BL/6 mice and 50 ug of CD137-Fc fusion protein per mouse was injected at day 1 after kidney IRI. The extent of renal injury, renal inflammation and tissue repair was analyzed using qRT-PCR and flow cytometry. Bone marrow-derived macrophages were stimulated with a copmbination of CD137-Fc abd GM-CSF and whole RNA sequencing was performed 24 hours after stimulation.

*Results: Firstly, injection of CD137-Fc fusion protein at day 1 after kidney IRI decreased renal inflammation and injury. These phenomena were associated with recruitment of a lower number of neutrophils and increase in the ratio of M2 macrophage-to-M1 macrophage. Secondly, administration of CD137-Fc enhanced expression of tissue repair genes, PDGFα, IL-22, Amphiregulin, IGF1, and TGF-β. Thirdly, there was a higher proliferation of tubular epithelial cells in CD137-Fc-injected mouse kidneys, concomitantly with increased expression of Vementin. Finally, ten days after kidney IRI, renal inflammation was almost completely resolved and renal macrophages showed a normal phenotype in CD137-Fc-injected mice. RNA seq data showed that CD137L signaling induced functionally different macrophages from naïve macrophages and GM-CSF and CD137L signaling synergistically increased expression of some anti-inflammatory and tissue-repair genes.

*Conclusions: In sum, our results indicate that CD137L signaling is indispensable for tissue repair following kidney IRI. Therefore, our findings have important clinical implications in kidney regeneration.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Cho HR, Lee S, Lee JS, Park SJ, Kwon B. Reverse Signaling through CD137 Ligand Promotes Tissue Repair after Ischemia-Reperfusion Kidney Injury [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/reverse-signaling-through-cd137-ligand-promotes-tissue-repair-after-ischemia-reperfusion-kidney-injury/. Accessed May 9, 2025.

« Back to 2019 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences