Date: Tuesday, May 2, 2017
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall D1
Background: The pro-/ anti-inflammatory balance in lung transplantation is shaped by the pulmonary immune compartments, i.e. the T and NK cell repertoire. At present, it is unknown whether the perfusion solution may influence the microenvironment of the lung. Therefore, perfusion solutions Perfadex (PER) and Celsior (CEL) after transplantation and peripheral blood of lung transplant recipients were analysed for their lymphocyte composition and their cytokine, chemokine and growth factor profile.
Methods: Lymphocytes from peripheral blood and perfusates of 42 lung transplant recipients (PER n=19 and CEL n=23), were isolated and T and NK cell subsets were analyzed by FACS; 70 cytokines, chemokines were quantified by protein multiplex analyses.
Results: Compared to the peripheral blood repertoire, lung-derived lymphocytes obtained from perfusates displayed increased frequencies of NK cells, CD69+ CD8+ and CD4+ T cells and KIR2DL1+, KIR2DL3+, KIR3DL1+ T and NK cells. In CEL perfusates, increased numbers of CD8+ T cells (p=0.023) and reduced CD4+ T cell numbers with an altered CD4/CD8 ratio (p=0.045) were observed compared to PER perfusates which correlated with increased CD8+ T cells, KIR2DL2+ KIR3DL1+ T cells in blood postoperatively in CEL recipients vs. PER recipients (p=0.0071). Moreover, higher CD69+ T and NK cells were detected in perfusates (p<0.01) indicating that they were originating from lung tissue. Regarding the protein microenvironment, IL-6, Ang-2 and VEGF-C levels were elevated in PER vs. CEL perfusates (p<0.05) which correlated postoperatively with significantly higher IL-6 levels in PER compared to CEL recipients (p=0.003).
Conclusion: Here, we unravel a novel mechanism of immune alterations subsequent to the application of perfusion solutions by changing composition of pulmonary T and NK cell subsets. In summary, lymphocyte subsets and cytokine release may be influenced by the perfusion solutions and potentially affect the pro-/ anti-inflammatory balance of the preserved organ. This impact on ischemia/reperfusion injury may be relevant for clinical outcome of the recipients.
CITATION INFORMATION: Bellmàs Sanz R, Seyda M, Wiegmann B, Neudoerfl C, Haverich A, Falk C, Warnecke G. Preservation Solution Affects the Immunological Milieu in Lung Transplantation and Alters the Inflammatory Balance. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Bellmàs R, Seyda M, Wiegmann B, Neudoerfl C, Haverich A, Falk C, Warnecke G. Preservation Solution Affects the Immunological Milieu in Lung Transplantation and Alters the Inflammatory Balance. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/preservation-solution-affects-the-immunological-milieu-in-lung-transplantation-and-alters-the-inflammatory-balance/. Accessed July 21, 2018.
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