Preservation Solution Affects the Immunological Milieu in Lung Transplantation and Alters the Inflammatory Balance.
1Institute of Transplant Immunology, IFB-Tx, Hannover Medical School, Hannover, Germany
2Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany
3German Center for Lung Research (DZL), Hannover, Germany
4German Center for Infection Research (DZIF), Hannover, Germany
Meeting: 2017 American Transplant Congress
Abstract number: D47
Keywords: Ischemia, Lung preservation, Lung transplantation
Session Information
Session Name: Poster Session D: Ischemic Injury and Organ Preservation Session III
Session Type: Poster Session
Date: Tuesday, May 2, 2017
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall D1
Background: The pro-/ anti-inflammatory balance in lung transplantation is shaped by the pulmonary immune compartments, i.e. the T and NK cell repertoire. At present, it is unknown whether the perfusion solution may influence the microenvironment of the lung. Therefore, perfusion solutions Perfadex (PER) and Celsior (CEL) after transplantation and peripheral blood of lung transplant recipients were analysed for their lymphocyte composition and their cytokine, chemokine and growth factor profile.
Methods: Lymphocytes from peripheral blood and perfusates of 42 lung transplant recipients (PER n=19 and CEL n=23), were isolated and T and NK cell subsets were analyzed by FACS; 70 cytokines, chemokines were quantified by protein multiplex analyses.
Results: Compared to the peripheral blood repertoire, lung-derived lymphocytes obtained from perfusates displayed increased frequencies of NK cells, CD69+ CD8+ and CD4+ T cells and KIR2DL1+, KIR2DL3+, KIR3DL1+ T and NK cells. In CEL perfusates, increased numbers of CD8+ T cells (p=0.023) and reduced CD4+ T cell numbers with an altered CD4/CD8 ratio (p=0.045) were observed compared to PER perfusates which correlated with increased CD8+ T cells, KIR2DL2+ KIR3DL1+ T cells in blood postoperatively in CEL recipients vs. PER recipients (p=0.0071). Moreover, higher CD69+ T and NK cells were detected in perfusates (p<0.01) indicating that they were originating from lung tissue. Regarding the protein microenvironment, IL-6, Ang-2 and VEGF-C levels were elevated in PER vs. CEL perfusates (p<0.05) which correlated postoperatively with significantly higher IL-6 levels in PER compared to CEL recipients (p=0.003).
Conclusion: Here, we unravel a novel mechanism of immune alterations subsequent to the application of perfusion solutions by changing composition of pulmonary T and NK cell subsets. In summary, lymphocyte subsets and cytokine release may be influenced by the perfusion solutions and potentially affect the pro-/ anti-inflammatory balance of the preserved organ. This impact on ischemia/reperfusion injury may be relevant for clinical outcome of the recipients.
CITATION INFORMATION: Bellmàs Sanz R, Seyda M, Wiegmann B, Neudoerfl C, Haverich A, Falk C, Warnecke G. Preservation Solution Affects the Immunological Milieu in Lung Transplantation and Alters the Inflammatory Balance. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:
Sanz RBellmàs, Seyda M, Wiegmann B, Neudoerfl C, Haverich A, Falk C, Warnecke G. Preservation Solution Affects the Immunological Milieu in Lung Transplantation and Alters the Inflammatory Balance. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/preservation-solution-affects-the-immunological-milieu-in-lung-transplantation-and-alters-the-inflammatory-balance/. Accessed May 12, 2025.« Back to 2017 American Transplant Congress