ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

PD-1+CD3+CD4-CD8- (Double Negative T-cells) Are Early Responders during Kidney Ischemia Reperfusion Injury and Regulated by Non-Classical MHC Molecules

M. Sadasivam, S. Noel, S. Lee, J. Gong, A. Hamad, H. Rabb

Johns Hopkins University and School of Medicine, Baltimore, MD

Meeting: 2019 American Transplant Congress

Abstract number: 81

Keywords: Interleukin-2 receptor, Ischemia, MHC class I, T cell activation

Session Information

Session Name: Concurrent Session: Ischemia Reperfusion & Organ Rehabilition I

Session Type: Concurrent Session

Date: Sunday, June 2, 2019

Session Time: 2:30pm-4:00pm

 Presentation Time: 3:42pm-3:54pm

Location: Room 313

*Purpose: Unconventional T-cells (e.g. Tregs and γδT-cells) are increasingly recognized for their roles in maintaining homeostasis and protecting non-lymphoid tissues against injury/stress. Double negative (DN) αβT-cells represent a significant component of T-cells in human and mouse kidneys, actively dividing in steady state and involved in protecting against kidney ischemia reperfusion injury (IRI). However, little is known about the regulation of kidney DN T-cells.

*Methods: C57BL/6J (WT), B6.129P2-B2mtm1Unc/J (β2m KO), B6.129S2-H2dlAb1-Ea/J (MHC II KO), B6.129P2-H2-K1tm1Bpe H2-D1tm1Bpe/DcrJ (KD KO) and Nu/J mice were used for this study, n=3-7/group. Human kidney samples were from the normal section of nephrectomies for renal cancers. Kidney lymphocytes were isolated and analyzed by flow cytometry.

*Results: Our data demonstrates that kidney DN T-cells are thymus-derived as lack of thymus significantly reduced the absolute count of DN T-cells in kidney (WT, 2.2×10 vs. Nu/J, 0.24×10 , P<0.05) and also comprised of two (PD-1 and NK1.1 ) subsets. Deficiency of β2m-dependent MHC class I, but not MHC class II molecules led to significant reduction in frequency, absolute numbers of kidney DN T-cells due to impaired activation (>60%, P<0.001), proliferation (>40%, P<0.05), increased apoptosis, and loss of a NK1.1 subset. Development of the NK1.1 subset is restricted by β2m-dependent nonclassical MHC molecules as the frequency of NK1.1 DN T-cells was significantly reduced in β2m KO (10.3±2) but not in KD KO or WT mice (23.1±3, P<0.001). In contrast, homeostasis but not development of the PD-1 subset is regulated by β2m-dependent non-classical MHC molecules as the frequency of PD-1 DN T-cells was significantly higher in β2m KO (70.2±5) then WT mice (44.2±3 P<0.001). DN T-cells retained in kidneys of β2m KO mice are mostly PD-1 and remain functionally responsive to external stimuli as indicated by their rapid activation and expansion in response to kidney IRI. Both normal human kidney and cancer samples express the PD-1 (% normal, 39.5±8 vs. tumor, 47.9±7.8) and NK1.1 subsets (% normal, 26.2±8.4 vs. tumor, 23.0±8.3).

*Conclusions: These findings demonstrate that kidney DN T-cells, particularly PD-1+ DN T-cells, are early responders of the renal immune surveillance system utilizing recognition/activation/ regulation mechanisms that differ from conventional T-cells.

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Sadasivam M, Noel S, Lee S, Gong J, Hamad A, Rabb H. PD-1+CD3+CD4-CD8- (Double Negative T-cells) Are Early Responders during Kidney Ischemia Reperfusion Injury and Regulated by Non-Classical MHC Molecules [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/pd-1cd3cd4-cd8-double-negative-t-cells-are-early-responders-during-kidney-ischemia-reperfusion-injury-and-regulated-by-non-classical-mhc-molecules/. Accessed June 1, 2025.

« Back to 2019 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences