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Optimal Development and Effector Function of Antibody-Suppressor CXCR5+CD8+ T Cells Requires Host IFN-γ and CD4+ T Cells

J. Zimmerer, S. Chaudhari, M. Hart, J. Han, K. Koneru, G. Bumgardner

OSUWMC, Columbus, OH

Meeting: 2022 American Transplant Congress

Abstract number: 1215

Keywords: Alloantigens, B cells, T cell activation

Topic: Basic Science » Basic Science » 04 - B-cell / Antibody /Autoimmunity

Session Information

Session Name: B-cell / Antibody /Autoimmunity

Session Type: Poster Abstract

Date: Monday, June 6, 2022

Session Time: 7:00pm-8:00pm

 Presentation Time: 7:00pm-8:00pm

Location: Hynes Halls C & D

*Purpose: We reported the discovery of a novel subset of CXCR5+IFNγ+CD8+ T cells that suppresses antibody production (CD8+ TAb-supp cells). Alloprimed CD8+ TAb-supp cells significantly inhibit posttransplant alloantibody production and prolong allograft survival. The requirements for CD8+ TAb-supp cell development are not understood. We hypothesized that CD4+ T cells and IFN-γ are required for the development of CD8+ TAb-supp cells following antigen stimulation.

*Methods: Wild-type (WT; C57BL/6) and IFN-γ KO knockout (KO) mice were alloprimed (FVB/N, H-2q, allogeneic lysate). Cohorts of mice were CD4-depleted and/or adoptively transferred (AT; day 0) with Violet CellTrace stained naïve CD8+ T cells (5 million) from GFP Tg mice. On day 7 post-stimulation, splenic CD8+ T cells were analyzed for the development of the CXCR5+CD8+ T cell phenotype, activation and in vivo proliferation (violet stain dilution). Alloprimed CXCR5+CD8+ T cells from WT and IFN-γ KO recipients were also evaluated for in vitro killing of alloprimed IgG+ B cells.

*Results: The proportion of CXCR5+CD8+ T cells increased significantly following allostimulation and was maximal on day 7 in WT control recipients (11.1±1.0%) compared to proportions on day 2 (3.7±1.0%), and day 5 after allostimulation (6.8±1.0%; p<0.01 for all time points) and in naïve mice (1.4±1.0%). CXCR5+CD8+ T cells express an activated phenotype (CD44+CD62L–) that peaks on day 7 (49.6±3.8%) compared to baseline before allopriming (2.3±0.7%; p<0.0001). To determine the role for host IFN-γ signaling and CD4+ T cells for the development of CXCR5+CD8+ T cells, we tracked the expression of CXCR5, activation markers, and in vivo proliferation. We found that the development of CXCR5+CD8+ T cells was significantly impaired in IFN-γ KO (6.3±1.0% of CD8+ T cells), CD4-depleted WT recipients (5.8±0.6%), and CD4-depleted IFN-γ KO (4.1±0.5%), compared to WT recipients (11.1±1.7%; p<0.02 for all), suggesting that host CD4+ T cells and host IFN-γ are critical for the development of alloprimed CXCR5+CD8+ T cells. Expression of activation marker CD44 and proliferation were similar for all experimental groups. Similar results for the development of endogenous CXCR5+CD8+ T cells were observed. IgG+ B cells were harvested from CD8 KO alloprimed hosts (day 7) and co-cultured with CXCR5+CD8+ T cells from different groups of alloprimed hosts to test for CD8-mediated in vitro cytotoxicity. WT CXCR5+CD8+ T cells exhibited significantly greater cytotoxicity (15.6±1.2%) compared to CXCR5+CD8+ T cells from CD4-deficient hosts (11.3±0.7%) or IFN-γ KO hosts (12.1±0.3%; p<0.04 for both).

*Conclusions: Host IFN-γ expression and CD4+ T cells are critical for optimal development and cytotoxic effector function of antibody-suppressor CXCR5+CD8+ T cells.

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To cite this abstract in AMA style:

Zimmerer J, Chaudhari S, Hart M, Han J, Koneru K, Bumgardner G. Optimal Development and Effector Function of Antibody-Suppressor CXCR5+CD8+ T Cells Requires Host IFN-γ and CD4+ T Cells [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/optimal-development-and-effector-function-of-antibody-suppressor-cxcr5cd8-t-cells-requires-host-ifn-%ce%b3-and-cd4-t-cells/. Accessed May 28, 2025.

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