ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Novel Effect of miR-26a:Mdm2:p53 Pathway on Mouse Liver Regeneration.

J. Zhou, W. Ju, X. Yuan, X. Jiao, D. Wang, X. Zhu, X. He.

Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.

Meeting: 2016 American Transplant Congress

Abstract number: D39

Keywords: Efficacy, Gene expression, Liver, Proliferation

Session Information

Session Name: Poster Session D: Chimerism/Stem Cells, Cellular/Islet Transplantation, Innate Immunity, Chronic Rejection

Session Type: Poster Session

Date: Tuesday, June 14, 2016

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Halls C&D

BACKGROUND: The deficiency of liver regeneration needs to be addressed in the fields of liver surgery, split liver transplantation and living donor liver transplantation. Researches of microRNAs would broad our understandings on various diseases mechanisms. Our previous research has confirmed that miR-26a regulated liver regeneration in mice, however, the relationship between miR-26a and its target Mdm2, directly or indirectly, was remain unclearly. Therefore, we further investigated the detailed mechanism of miR-26a regulating mouse hepatocyte proliferation in the current study.

METHODS: The established mouse liver cell line, Nctc-1469, was cultured in vitro. The relationship between miR-26a and Mdm2 were assessed by dual-luciferase reporter assays. The effect of Mdm2, p53, as well as co-transfection of miR-26a and Mdm2 on cell proliferation and apoptosis were examined through flow cytometry. RT-qPCR and Western Blot were used to examine the expression of miR-26a and p53 in Mdm2 knockdown or over-expressed cells. The expressions of Mdm2, p53, caspase3, cyclinD1, p21 and p27 were assessed by Western Blot when miR-26a expression was inhibited or enhanced, as well as when miR-26a and Mdm2 were co-transfected.

RESULTS: Our results showed that Mdm2 was the direct targeted gene of miR-26a, in miR-26a downregulated cells, the expressions of Mdm2 and cyclinD1 were increased, while p53, caspase3, p21 and p27 were downregulated. When Mdm2 gene was overexpressed, the expressions of miR-26a and p53 were downregulated, the overexpression of Mdm2 gene promoted the proliferation of hepatocytes and inhibited cell apoptosis. The p53 gene inhibited the proliferation of hepatocytes and increased cell apoptosis. In the miR-26a and Mdm2 co-transfected hepatocytes, when expression of miR-26a was inhibited accompanied by upregulated Mdm2 gene, the proliferation of hepatocytes was increased and cell apoptosis was inhibited, the expressions of Mdm2 and cyclinD1 were increased, and p53, caspase3, p21 and p27 were downregulated. Meanwhile, previous study has confirmed the negative feedback regulation of p53 and Mdm2.

CONCLUSIONS: This study demonstrated that miR-26a regulated mouse hepatocyte proliferation by miR-26a:Mdm2:p53 pathway. It is suggested that miR-26a:Mdm2:p53 play a novel effect on mouse liver regeneration.

CITATION INFORMATION: Zhou J, Ju W, Yuan X, Jiao X, Wang D, Zhu X, He X. Novel Effect of miR-26a:Mdm2:p53 Pathway on Mouse Liver Regeneration. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Zhou J, Ju W, Yuan X, Jiao X, Wang D, Zhu X, He X. Novel Effect of miR-26a:Mdm2:p53 Pathway on Mouse Liver Regeneration. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/novel-effect-of-mir-26amdm2p53-pathway-on-mouse-liver-regeneration/. Accessed June 1, 2025.

« Back to 2016 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences