Molecular Characterization of the Plasma Secretome in Pediatric Liver Transplantation (pLTx) Identified Cytokine/Chemokine Signatures Potentially Linked to Beneficial Immunosuppressive Regimes (IS) and Recovery
1Institute of Transplant Immunology, Hannover Medical School (MHH), Hannover, Germany, 2Institute of Transplant Immunology, MHH, Hannover, Germany, 3Division of Pediatric Gastroenterology and Hepatology, MHH
European Paediatric Liver Transplantation Network, Hannover, Germany, 4Research Group Epidemiological and Statistical Methods, Helmholtz Centre for Infection Research, Braunschweig
German Center for Infection Research, TTU-IICH Hannover;Institute for Epidemiology and Social Medicine, University of Münster, Münster, Germany, 5Ospedali Riuniti di Bergamo, Bergamo, Italy, 6Hôpital Necker-Enfants Malades, Paris, France, 7Hospital Infantil Universitario La Paz, Madrid, Spain, 8Birmingham Childrens Hosp, Birmingham, United Kingdom, 9Service Spécialités Pédiatriques, Genève, Switzerland, 10Pediatric Hepatology, Gastroenterology and Transplantation, Hospital Papa Giovanni XXIII, Bergamo, Italy, 11Gastroenterology, Hepatology, Nutritional Disorders and Pediatrics, The Children’s Memorial Health Institute, Warsaw, Poland, 12Institute of Medical Epidemiology, Biostatistics and Medical Informatics, University of Halle
German Center for Infection Research, TTU-IICH Hannover
MHH, Halle, Germany, 13Division of Pediatric Gastroenterology and Hepatology, MHH
Birmingham Children’s Hospital, UK, Hannover, Germany, 14Institute of Transplant Immunology, MHH
German Center for Infection Research, TTU-IICH, Hannover, Germany
Meeting: 2022 American Transplant Congress
Abstract number: 1444
Keywords: Immunogenicity, Immunosuppression, Liver transplantation, Rejection
Topic: Clinical Science » Liver » 61 - Liver: Pediatrics
Session Information
Session Time: 7:00pm-8:00pm
Presentation Time: 7:00pm-8:00pm
Location: Hynes Halls C & D
*Purpose: In the European multicentre “ChilSFree” study we aimed to characterize longitudinal dynamics of soluble immune mediators during the first year after pLTx.
*Methods: Using Luminex-based multiplex technique, we measured 50 cytokines/chemokines, growth and adhesion factors in recipient plasma at visits: before (V0), 1 (V1), 2 (V2), 3 (V3), 4 (V4) weeks, 3 (V5), 6 (V6) months and 1 year after pLTx (n>205).
*Results: Multigroup comparisons revealed main changes in plasma secretome to appear within V0-3, while later visits were clustered by their secretome signatures independently on the visit kinetics, likely reflecting different recovery patterns. For instance, one of these secretome signatures was characterized by the absence of pro-inflammatory CXCL8/9/10/12, CCL7, IL-17 mediators and reduced liver enzyme (ALT, AST, GGT) levels, and, hence, might be predictive for a better outcome after pLTx. We also identified IS regimes which were linked to diminished inflammation and rejection rates. Some of them showed sex-dependent patterns.
*Conclusions: Plasma secretome signatures might act as a biomarker for potential outcome after pLTx. This information might be useful for improved therapeutic options.
To cite this abstract in AMA style:
Chichelnitskiy E, Ruhl L, Goldschmidt I, Karch A, Antiga L, Debray D, Hierro L, Kelly D, McLin V, Nicastro E, Pawlowska J, Czubkowski P, Mikolajczyk R, Baumann U, Falk C. Molecular Characterization of the Plasma Secretome in Pediatric Liver Transplantation (pLTx) Identified Cytokine/Chemokine Signatures Potentially Linked to Beneficial Immunosuppressive Regimes (IS) and Recovery [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/molecular-characterization-of-the-plasma-secretome-in-pediatric-liver-transplantation-pltx-identified-cytokine-chemokine-signatures-potentially-linked-to-beneficial-immunosuppressive-regimes-is-an/. Accessed May 23, 2025.« Back to 2022 American Transplant Congress