Objective: To explore the distribution characteristics of MICA/B gene polymorphism in the Han Chinese and the relationship of the matching degree between MICA/MICB and HLA of related donor-recipient pairs in allo-HSCT.

Methods: The MICA/B gene polymorphism research samples were consisted of 302 randomly selected unrelated healthy volunteers. The related donor-recipient groups was consisted of 300 leukemia patients and 401 related potential donors for allo-HSCT, which included 148, 135 and 115 pairs of HLA matched, HLA semi-matched and HLA fully-mismatched related donor-recipients, respectively. All the above sample groups had been genotyped for their HLA-A, -B, -C, -DRB1, -DQB1 loci at Beijing 307 hospital from 2002 to 2008. PCR-SSP method was performed to genotype MICA and MICB loci. Statistical analyses were performed to study the distribution of MICA/B polymorphism and the relationship of matching degree between MICA/B and HLA of related donor-recipient pairs in allo-HSCT.

Results: Nineteen MICA alleles and 11 MICB alleles were detected in the Han Chinese. The allelic frequencies greater than 10% of MICA and MICB were MICA* 008:01/04, MICA* 010, MICA* 002:01, MICB* 005:02/010 and MICB* 002:01, respectively. We identified 17 MICA-HLA-B and 17 MICB-HLA-B haplotypes, among which there were 17 MICA-HLA-B and 13 MICB HLA-B haplotypes in significant linkage disequilibrium, and the preponderance haplotypes were MICA*045-B*13, MICA*012:01-B*54, MICA*002:01-B*58, MICA*010-B*46, MICA*009:01-B*52, MICB*008-B*58, MICB*009N-B*48 and MICB*005:02/010-B*46. We found that the matching degree of MICA and MICB is closely related to that of HLA between donor-recipient pairs, and the matching degree of MICA is 95.9%, 87.4% and 56.8% in HLA matched, HLA semi-matched and HLA fully-mismatched donor-recipient groups, respectively. And the similar tendency was also found in MICB with the matching degree of 95.9%, 66.7% and 31.4% in the above 3 HLA matching degree groups, respectively.

Conclusions: This study revealed the high polymorphism of MICA/B and linkage disequilibrium parameters of MICA-HLA-B and MICB-HLA-B haplotypes of the Han Chinese, and also demonstrated the closely consistent matching status of MICA/B and HLA indicating the importance of matching MICA/B when choosing the optimal potential donors for allo-HSCT.

CITATION INFORMATION: Sun Y., Hao Y., Jin J., Ding L., Liang F., Liu N., Long J., Zhao X., Xi Y. MICA/B Polymorphisms and the Relationship of the Matching Degree between MICA/B and HLA of Related Donor-Recipient Pairs in Allo-HSCT Am J Transplant. 2017;17 (suppl 3).

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