Metabolomic Analysis of Remote Ischemic Preconditioning in Renal Ischemia Reperfusion Injury
1Department of Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea
2Depratment of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine, Seoul, Republic of Korea
3Depratment of Surgery, Myongji Hospital, Goyang-si, Gyeonggi-do, Republic of Korea
4Korean Organ Donation Agency, Seoul, Republic of Korea.
Meeting: 2015 American Transplant Congress
Abstract number: C233
Keywords: Ischemia
Session Information
Date: Monday, May 4, 2015
Session Name: Poster Session C: Translational Biomarkers and Immune Monitoring
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Background. Ischemic reperfusion injury (IRI), an inevitable process during kidney transplantation, can cause significant graft damage and may play a role in delayed graft function. Although remote ischemic preconditioning (RIPC) is suggested to reduce IRI, its precise molecular mechanism is not well understood and clinically applicable biomarkers of both IRI and RIPC are lacking.
Methods. We have performed a metabolomic analysis of RIPC in renal IRI model. Renal ischemia reperfusion injury was achieved by clamping the renal vascular pedicle for 45 minutes, and declamping for 72hours. RIPC protocol consisted of three periods of 5 minute Left hind leg ischemia separated by 5 minutes of reperfusion. RIPC was given 24 hours before renal IRI in early RIPC group (n=8), and 2 hours before in late RIPC group (n=8). No RIPC were given to IRI only group (n=7) and only sham procedure was performed in sham group (n=7). By using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS), with multivariate statistical analysis, metabolomic profiles of urine blood tissue samples were analyzed.
Results. Both late RIPC group and early RIPC group showed significantly less acute tubular injury than IRI only group. (Acute tubular injury score, Late RIPC 1.63±0.92 group, Early RIPC group 1.38±0.92, IRI only group 3.43±0.79, P<0.001). Metabolites that showed increase in IRI only group, and showed significantly less increase in RIPC group were oxo-pentyl-cyclopentaneoctanoic acid, eicosatrienoic acid, eicosatrienoic acid, lyso PC (14:1), PIP2 (22:2/16:0), lyso PC (24:0), glucosylceramide (18:1/16:0), PE (18:1/20:1). 5-L-glutamyl-taurine, 2-octenoylcarnitine, inosine-5'-carboxylate, ceramide (18:1/16:0), PE (20:3/20:4) showed decrease in IRI only group, and less decrease in RIPC group.
Conclusion. Metabolomic analysis seems feasible in detecting changes associated with IRI and RIPC, and thus may well be applied to human studies. Such approach could identify potential biomarkers of IRI that can be measured non- or minimally invasively
To cite this abstract in AMA style:
Han A, Min S-I, Cho K, Choi C, Min S-K, Kim S, Park M, Cho J-Y, Ha J. Metabolomic Analysis of Remote Ischemic Preconditioning in Renal Ischemia Reperfusion Injury [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/metabolomic-analysis-of-remote-ischemic-preconditioning-in-renal-ischemia-reperfusion-injury/. Accessed November 29, 2020.« Back to 2015 American Transplant Congress