ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Local Intragraft Humoral Immune Response in Chronic Lung Allograft Dysfunction

E. Miyamoto1, S. C. Juvet2, D. Vosoughi2, J. Wang2, J. Al-Refaee2, T. Daigneault2, A. Duong2, S. Moshkelgosha2, S. Keshavjee2, K. Tinckam2, A. Chruscinski3, D. Hwang4, T. Martinu2

1Tenri Hospital Japan, University Health Network Canada, Nara, Japan, 2University Health Network, Toronto, ON, Canada, 3Multi-Organ Transplantation, University Health Network, Toronto, ON, Canada, 4Sunnybrook Health Science Centre, Toronto, ON, Canada

Meeting: 2022 American Transplant Congress

Abstract number: 1492

Keywords: Autoimmunity, B cells, HLA antibodies, Rejection

Topic: Clinical Science » Lung » 64 - Lung: All Topics

Session Information

Session Name: Lung Transplantation

Session Type: Poster Abstract

Date: Monday, June 6, 2022

Session Time: 7:00pm-8:00pm

 Presentation Time: 7:00pm-8:00pm

Location: Hynes Halls C & D

Session Information

Session Name: Poster Chat: Heart and Lung

Session Type: Poster Chat

Date: Tuesday, June 7, 2022

Session Time: 7:00pm-8:00pm

 Presentation Time: 7:00pm-8:00pm

Location: Hynes Hall C

*Purpose: Donor HLA-specific antibody (DSA) and antibody against self-antigens (self-antigen antibody) can cause injury and lead to chronic lung allograft dysfunction (CLAD) after lung transplantation (LTx). Despite increasing interest in the role of antibodies in lung transplantation, it remains uncertain whether these are produced locally in the graft or primarily remain in the circulation. We aimed to determine whether DSA and antibodies directed at self antigens are produced in CLAD lungs.

*Methods: Fresh whole lungs were prospectively collected from 15 LTx patients who underwent re-transplant or medically assisted death due to CLAD. 0.3g of fresh lung tissue was cultured for 4 days with or without lipopolysaccharide or CD40L. Lung culture supernatant (LCS) was sampled. Lung eluate was obtained from 0.3g of frozen lung tissue. Lung B and plasma cells were analyzed by flow cytometry. Mean fluorescent intensity (MFI) of DSA and self-antigen antibodies were measured by Luminex and antigen microarray, respectively. Lymphoid aggregates (LAs) in the lung graft were assessed by hematoxylin-eosin staining and immunofluorescence.

*Results: CD40L-stimulated LCS from all 4 patients who had serum DSA at lung isolation were positive for DSA, while the matched lung eluate were positive for DSA only in 3 of those patients. (Fig. A). Antibodies against self-antigens were also detectable in LCS and lung eluates (Fig. B). MFIs of IgG against self-antigens of lung eluates correlated with those of CD40L-stimulated LCS but not those of other LCS or serum (Fig. C). Patients whose CD40L-stimulated LCS was positive for DSA or self-antigen antibody (local antibody) showed significantly higher proportions of activated (CD27+CD38+, AID+, or CD86+) B cells and plasma cells in the lung tissue compared to those who had no local antibody in LCS (Fig. D). LAs of patients with local antibody production were surrounded by significantly higher numbers of IgG+ and IgM+ plasma cells (Fig. E), with larger IL-21+ fractions.

*Conclusions: Donor lung graft itself is capable of producing DSA and self-reactive antibodies. Further investigation is required to demonstrate the clinical relevance of locally produced antibodies in the development of CLAD.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Miyamoto E, Juvet SC, Vosoughi D, Wang J, Al-Refaee J, Daigneault T, Duong A, Moshkelgosha S, Keshavjee S, Tinckam K, Chruscinski A, Hwang D, Martinu T. Local Intragraft Humoral Immune Response in Chronic Lung Allograft Dysfunction [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/local-intragraft-humoral-immune-response-in-chronic-lung-allograft-dysfunction/. Accessed May 9, 2025.

« Back to 2022 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences