ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Inflammatory Profiling of Hypothermic Machine Pumped Kidney Allografts

J. Cohen, E. Ratigan, A. Shigeoka, R. Steiner, L. Stocks, D. McKay.

Medicine, University of California, San Diego, La Jolla, CA.

Meeting: 2015 American Transplant Congress

Abstract number: C273

Keywords: Inflammation, Ischemia, Perfusion solutions

Session Information

Session Name: Poster Session C: Translational Biomarkers and Immune Monitoring

Session Type: Poster Session

Date: Monday, May 4, 2015

Session Time: 5:30pm-6:30pm

 Presentation Time: 5:30pm-6:30pm

Location: Exhibit Hall E

Background:

Pulsatile Hypothermic Machine Perfusion (MP) offers an opportunity to define inflammatory molecules generated during renal ischemia. This study reports on allografts pumped at our OPO, Lifesharing®, in which serial perfusate samples were analyzed for time-dependent generation of inflammatory mediators.

Methods:

Perfusate of 17 kidneys from non “high risk” kidney donors was analyzed over time using Biorad Bio-plex Pro Human Cytokine and RBM Human Kidney Toxicity 1 assays® for IL-1b, IL-1Ra, IL-2, IL-4, IL-6, IL-10, IFNg, MCP-1, MIP1a, TNFa, IL-18, MCP-1, and Kim-1. Inflammatory mediators were categorized at 4 hours (as low versus high secretors) and correlated with average on-pump resistance.

Results:

Amongst inflammatory molecules, only MCP-1 demonstrated a significant time-dependent increase. IFNγ and Interleukin-6 trended to an increase, whereas all other inflammatory molecules did not increase over time.

Table 1: Generation of inflammatory mediators over time. Results are presented as pg/mg of total protein. Only cytokines that increased from time 0 (pre-pump flush) are included in the table. Errors represent SEM.

n=17 IFN-γ IL-6 MCP-1
0 hr 20 +/-1 2 +/- 0.06 90+/- 5
2 hr 26 +/-1 6 +/- 0 133+/- 4
4 hr 25+/- 1 7+/- 0 151+/- 6

Of cytokines that increased over time, only IL-6 correlated with high vascular resistance (p=0.02).

Table 2: Mean on-pump resistive indices between lowest and highest secretors of IFNγ, IL-6, and MCP-1.

  Low Secretor High Secretor p-value
IL-6 0.20 (+/- 0.06) 0.30 (+/- 0.05) 0.005
IFN-γ 0.25 (+/- 0.09) 0.26 (+/- 0.08) 0.700
MCP-1 0.25 (+/- 0.08) 0.25 (+/- 0.08) 0.975

Conclusions: Kidney allografts on-pump significantly released MCP-1 and trended to higher release of IFNγ and IL-6 over time, but only IL-6 secretion was correlated with high resistive indices. These data suggest MCP-1, as well as IFNγ and IL-6, may play an important trigger for post-transplant inflammation. The association of IL-6 with on-pump resistive indices suggests that IL-6 may serve as an important biomarker for high pump resistance. Our laboratory is currently evaluating the relevance of these findings to renal transplant outcomes.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Cohen J, Ratigan E, Shigeoka A, Steiner R, Stocks L, McKay D. Inflammatory Profiling of Hypothermic Machine Pumped Kidney Allografts [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/inflammatory-profiling-of-hypothermic-machine-pumped-kidney-allografts/. Accessed May 9, 2025.

« Back to 2015 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences