Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall D1
Aim: Long term allograft survival has not improved.Surveillance biopsy studies have demonstrated that chronic microcirculatory injury and progressive glomerulosclerosis are the most common histological finding in aging allografts (Nankivell, 2003)(El-Zoghby, 2008) and together may be contributing to late allograft loss.Podocytes are post-mitotic cells and their depletion causes glomerulosclerosis. We have recently shown that allografts lose podocytes at a six-fold higher rate compared to native kidneys (Yang,2015).However, the causes of increased podocyte loss in allografts are not well understood. In this study we assess the impact of type of kidney on podocyte detachment rate (PDR).
Methods: Urine specimens were collected from patients presenting for their surveillance biopsy at months 3, 6 and 12 months after transplantation. PDR was calculated by measuring Podocin mRNA to creatinine ratio. Demographic, clinical and transplantation factors were collected from recipients and donors.Unpaired t test was used to assess the difference in average podocyte detachment rate at 3, 6 and 12 months post transplantation between living (LD) and deceased (DD) donors.
|Time after Txp||3 mo||3 mo||6 mo||6 mo||12 mo||12 mo|
|Average (10-5) PDR||2.9||5.8**||2.1||3.5**||2.5||2.7|
|Std. Error (10-5)||0.3||1.5||0.3||0.7||0.4||1.3|
** P value < 0.05
|Time after TXP||3 mo||3 mo||6 mo||6 mo||12 mo||12 mo|
|Cold Ischemia Time||CIT < 12||CIT ≥12||CIT < 12||CIT ≥12||CIT <12||CIT ≥12|
|Average (10-5) PDR||2.4||4.7||2.8||3.7||1.6||1.4|
|Std. Error (10-5)||1.0||1.0||1.0||0.9||0.4||0.3|
Conclusion: Early after transplantation kidneys from LD appear to have a lower urinary podocyte excretion rate.There was no statistical difference in PDR among DD allografts by degree of CIT. The difference in PDR between LD and DD may related to higher warm-time in DD or lower podocyte density in donated kidney, but needs further investigation. Understanding factors that will help maintain podocyte density after transplantation may allow improvement in long term allograft survival.
CITATION INFORMATION: Naik A, Luan D, Cibrik D, Samaniego M, Woodside K, Chowdhury M, Wang S, Wiggins R. Impact of Type of Kidney (Living vs. Deceased) on Urinary Podocyte Excretion Rate in Renal Allografts. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Naik A, Luan D, Cibrik D, Samaniego M, Woodside K, Chowdhury M, Wang S, Wiggins R. Impact of Type of Kidney (Living vs. Deceased) on Urinary Podocyte Excretion Rate in Renal Allografts. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/impact-of-type-of-kidney-living-vs-deceased-on-urinary-podocyte-excretion-rate-in-renal-allografts/. Accessed October 1, 2020.
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