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Impact of Extracorporeal Photopheresis in a Full-Mismatch Kidney Transplant Rat Model

J. Rovira1, G. J. Piñeiro2, M. Lazo-Rodriguez3, M. J. Ramirez-Bajo1, E. Banon-Maneus3, N. Hierro-Garcia3, P. Ventura-Aguiar2, J. Cid4, M. Lozano4, F. Diekmann2

1Laboratori Experimental de Nefrologia i Trasplantament (LENIT), IDIBAPS, Barcelona, Spain, 2Department of Nephrology and Renal Transplantation, Hospital Clínic de Barcelona, Barcelona, Spain, 3Laboratori Experimental de Nefrologia i Trasplantament (LENIT), Fundació Clínic per la Recerca Biomèdica (FCRB), Barcelona, Spain, 4Apheresis Unit, Department of Hemotherapy and Hemostasis, Hospital Clínic de Barcelona, Barcelona, Spain

Meeting: 2020 American Transplant Congress

Abstract number: D-296

Keywords: Kidney transplantation, Rat, Rejection, Survival

Session Information

Date: Saturday, May 30, 2020

Session Name: Poster Session D: Cellular Therapies, Tissue Engineering / Regenerative Medicine

Session Time: 3:15pm-4:00pm

 Presentation Time: 3:30pm-4:00pm

Location: Virtual

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*Purpose: Extracorporeal photopheresis (ECP) is an immunomodulatory cell therapy based on the infusion of autologous cellular products exposed to ultraviolet light (UV), in the presence of a photosensitizer. Nowadays, the three main indications for ECP therapy are cutaneous T-cell lymphoma, graft-versus-host disease and pulmonary or heart transplant rejection. The aim of the present study is to evaluate ECP therapy for the prevention of rejection in full mismatch kidney transplant rat model.

*Methods: We have established the Dark Agouti to Lewis (DA-LEW) full-mismatch kidney transplant model. Calcineurin inhibitor, tacrolimus, was administered at different doses for 4 days. ECP product was obtained from LEW recipients at day 4 after DA kidney graft transplantation. Peripheral blood and spleen of those LEW rats were processed until cell suspension, which was exposed to 8-methoxypsoralen (300 ng/mL) and illuminated with UV-A light (2 J/cm2). Seven doses of ECP were administered to LEW recipients, the first doses at day 14 before transplantation, and the last one at day 14 after transplantation.

*Results: DA-LEW kidney transplant model was characterized by renal function deterioration from day 3 and progressed rapidly until graft loss and rat death, with a mean survival time (MST) of 6.125±0.45 days. Donor specific antibodies were detected from day 3. Kidney graft histological analysis showed cortical necrosis, peritubular capillaritis, thrombotic microangiopathy, interstitial infiltrated, mainly by polymorphonuclear leukocytes and macrophages, and C4d deposition on peritubular capillaries. Short treatment with Tacrolimus (0.25mg/kg) prolonged rat survival until 11.4±0.70 days (P=0.0004). The combination of Tacrolimus and ECP therapy extended kidney graft survival until 26.28±7.3 days (P=0.0470).

*Conclusions: ECP is a suitable immunomodulatory therapy to be explored in the field of kidney transplantation.

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To cite this abstract in AMA style:

Rovira J, Piñeiro GJ, Lazo-Rodriguez M, Ramirez-Bajo MJ, Banon-Maneus E, Hierro-Garcia N, Ventura-Aguiar P, Cid J, Lozano M, Diekmann F. Impact of Extracorporeal Photopheresis in a Full-Mismatch Kidney Transplant Rat Model [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/impact-of-extracorporeal-photopheresis-in-a-full-mismatch-kidney-transplant-rat-model/. Accessed April 18, 2021.

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