Identification of Soluble and Cellular Biomarkers in Blood of End-Stage Patients Before Pediatric Liver Transplantation (pLTx)
1Institute of Transplant Immunology, Hannover Medical School (MHH), Hannover, Germany, 2Institute of Transplant Immunology, MHH, Hannover, Germany, 3Division of Pediatric Gastroenterology and Hepatology, MHH
European Paediatric Liver Transplantation Network, Hannover, Germany, 4Research Group Epidemiological and Statistical Methods, Helmholtz Centre for Infection Research, Braunschweig
German Center for Infection Research, TTU-IICH Hannover
Institute for Epidemiology and Social Medicine, University of Münster, Münster, Germany, 5Ospedali Riuniti di Bergamo, Bergamo, Italy, 6Hôpital Necker-Enfants Malades, Paris, France, 7Hospital Infantil Universitario La Paz, Madrid, Spain, 8Birmingham Children's Hospital, Birmingham, United Kingdom, 9Service Spécialités Pédiatriques, Genève, Switzerland, 10Gastroenterology, Hepatology, Nutritional Disorders and Pediatrics, The Children’s Memorial Health Institute, Warsaw, Poland, 11German Center for Infection Research, TTU-IICH Hannover
Institute of Medical Epidemiology, Biostatistics and Medical Informatics, University of Halle, Halle, Germany, 12Division of Pediatric Gastroenterology and Hepatology, MHH
Birmingham Children’s Hospital, UK
European Paediatric Liver Transplantation Network, Hannover, Germany, 13Institute of Transplant Immunology, MHH
German Center for Infection Research, TTU-IICH, Hannover, Germany
Meeting: 2022 American Transplant Congress
Abstract number: 1452
Keywords: Immunogenicity, Liver transplantation, Lymphocytes, Risk factors
Topic: Clinical Science » Liver » 61 - Liver: Pediatrics
Session Information
Session Time: 7:00pm-8:00pm
Presentation Time: 7:00pm-8:00pm
Location: Hynes Halls C & D
*Purpose: In the European multicentre “ChilSFree” study we aimed to identify soluble and cellular biomarkers in blood of various end-stage diseases before pLTx.
*Methods: Using flow cytometry-based immunophenotyping, and protein multiplex (50-plex) array we defined cellular and soluble immune characteristics of 10 major end-stage diseases before pLTx (n>153).
*Results: The Alagille syndrome showed the highest count of almost all immune cells: CD3+T cells, CD19+B cells, CD56+NK cells, granulocytes and monocytes as well as higher plasma concentrations of CXCL10, CCL4, PDGF-b, FGF, IL-13, IL-9. Hepatic malignancy was demarcated by the highest ratio of CD3+T cells, comprising nearly 90% of all lymphocytes in these patients as well as increased monocytes ratio, while acute liver failure was defined by the altered CD4+/CD8+ T cell ratio and higher counts of granulocytes and monocytes and CXCL10 and HGF plasma levels. The immune profile of biliary atresia was dominated by granulocytes and monocytes as well as higher CXCL1, HGF, CXCL8 and CCL4.
*Conclusions: We provide here soluble and cellular blood immune characteristics of major end stage diseases before pLTx, which may pave the way to improved therapeutic options.
To cite this abstract in AMA style:
Chichelnitskiy E, Ruhl L, Goldschmidt I, Karch A, Antiga L, Debray D, Hierro L, Kelly D, McLin V, Nicastro E, Pawlowska J, Mikolajczyk R, Baumann U, Falk C. Identification of Soluble and Cellular Biomarkers in Blood of End-Stage Patients Before Pediatric Liver Transplantation (pLTx) [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/identification-of-soluble-and-cellular-biomarkers-in-blood-of-end-stage-patients-before-pediatric-liver-transplantation-pltx/. Accessed May 6, 2025.« Back to 2022 American Transplant Congress