Date: Monday, June 4, 2018
Session Time: 4:30pm-6:00pm
Presentation Time: 5:06pm-5:18pm
Location: Room 606/607
Introduction: The gut microbiota induce and train the host immune system. Whether microbiota from contrasting environments of colitic or pregnant mice are pro- or anti-inflammatory is unknown. We hypothesized that gut microbiotas from different sources could influence the survival of murine cardiac allografts.
Methods: C57BL/6 mice received BALB/c vascularized cardiac allografts and fecal microbiota transfer (FMT) by oral gavage. FMT samples were from normal or pregnant C57BL/6, spontaneously colitic mice, or cultured Bifidobacterium pseudolongum (Bifido) (a dominant member of the pregnant gut microbiota). Tacrolimus was administered daily. Fecal pellets were collected weekly post-transplant and analyzed by 16S rRNA gene sequencing. Grafts were harvested at days 40-60 or rejection, and assessed for inflammation by H&E and fibrosis by Masson's trichrome. Macrophage (MF) and dendritic cell (DC) lines were stimulated with purified Bifido cells and cytokine responses measured.
Results: Pregnant FMT enhanced cardiac allograft survival, resulting in reduced inflammation and fibrosis. Normal or colitic FMT resulted in inferior survival, along with increased fibrosis and inflammation. 16S rRNA analyses demonstrated abundant Bifido in the pregnant and normal but not the colitic FMT samples. Transfer of Bifido alone also resulted in enhanced allograft survival and reduced inflammation and fibrosis. Serial 16S rRNA analyses of gut microbiota from normal, colitic, pregnant FMT, or Bifido groups, revealed significant differences in bacterial community structure (alpha- and beta-diversity). Bifido remained abundant in pregnant FMT recipient samples for at least 40 days. In contrast, Desulfovibrio and Deferribacteraceae were more abundant in colitic samples. Microbiota community structures of the various groups became less different over time, but remained distinct. DC and MF lines stimulated with Bifido expressed higher levels of the anti-inflammatory cytokine IL-10 and homeostatic chemokine CCL19, but less pro-inflammatory cytokines TNFa and IL-6 compared to LPS stimulated cells.
Conclusion: The gut microbiota has profound effects on host immune responses with consequences for cardiac allograft outcomes. Distinct bacterial species or genera may both alter immunity and predict graft outcomes, with Bifidobacterium species inducing anti-inflammatory effects through stimulation of DC and MF. Therapeutic targeting of the microbiota or its functions could be beneficial in reducing inflammation and subsequent graft rejection.
CITATION INFORMATION: Xiong Y., Brinkman C., Hittle L., Fricke W., Mongodin E., Bromberg J. Identification of Anti-Inflammatory and Pro-Inflammatory Gut Microbiota That Determine Graft Survival Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Xiong Y, Brinkman C, Hittle L, Fricke W, Mongodin E, Bromberg J. Identification of Anti-Inflammatory and Pro-Inflammatory Gut Microbiota That Determine Graft Survival [abstract]. https://atcmeetingabstracts.com/abstract/identification-of-anti-inflammatory-and-pro-inflammatory-gut-microbiota-that-determine-graft-survival/. Accessed July 11, 2020.
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