High Regulatory T Cells Rate Early After Lung Transplantation Is Associated with Bronchiolitis Obliterans Syndrome Development.
1INSERM U 1064, Center for Research in Transplantation and Immunology (CRTI), Nantes, France
2Université
de Nantes, Nantes, France
3UMR INSERM 1087 CNRS 6291, Institut du Thorax, Nantes, France
4Service de Pneumologie, Institut du Thorax, Nantes, France
Meeting: 2017 American Transplant Congress
Abstract number: 82
Keywords: FACS analysis, Lung transplantation, Obilterative bronchiolitis, T cells
Session Information
Session Name: Concurrent Session: Predicting Tolerance and Rejection
Session Type: Concurrent Session
Date: Sunday, April 30, 2017
Session Time: 2:30pm-4:00pm
Presentation Time: 2:30pm-2:42pm
Location: E351
Despite treatment improvement, the bronchiolitis obliterans syndrome (BOS) affects more than 50% of the lung-transplant recipient in the 5 years post-transplantation and because it cannot be predicted or cured, it is the major cause of death after lung transplantation (TP).
The COLT (Cohort in Lung Transplantation), initiated in order to understand the physiopathology of chronic lung allograft dysfunction, gave us the opportunity to follow the immune profile of the patients from the TP to the BOS occurrence. The lymphocyte profile of 34 stable (STA) and 27 BOS patients was investigated before TP, in the 6 months after TP, 6 months before BOS and at the BOS diagnosis. PBMCs were analysed by flow-cytometry with “classical” T-cells and regulatory T cells (Tregs) markers.
Whereas no significant difference for CD4 and CD8 T cells subsets was observed between BOS and STA patients at the different time points, an increase in FoxP3+ Tregs proportion was observed in the 6 months post-TP in BOS compared to STA (0,85% and 3,36% in STA and BOS respectively, p<0,005). FoxP3+ Tregs were mainly memory Tregs (CD45RA– and CD39+). In the whole study, the value 2,08 % was computed as cut-off for the FoxP3+ Tregs proportion in the six months post-TP able to distinguish patients who will declare a BOS in the 5 years (area under ROC curve 0,745 ; p<0,005 ; 70% specificity ; 72,41% sensitivity). Patients with a low proportion of Tregs, under 2,08%, early after transplantation, exhibit a significant increase in long-term BOS-free survival, compared to those with more than 2,08% of Tregs after TP (41% and 75% in hiTregs and lowTregs respectively, p<0,01).
In conclusion, our study leads to identify CD4+ FoxP3+ Regulatory T cells proportion as new potential predictive biomarker of the long-term BOS development after lung transplantation.
CITATION INFORMATION: Durand M, Lacoste P, Brosseau C, Durand E, Loy J, Henrio K, Danger R, Royer P, Fourreau A, Magnan A, Brouard S. High Regulatory T Cells Rate Early After Lung Transplantation Is Associated with Bronchiolitis Obliterans Syndrome Development. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:
Durand M, Lacoste P, Brosseau C, Durand E, Loy J, Henrio K, Danger R, Royer P, Fourreau A, Magnan A, Brouard S. High Regulatory T Cells Rate Early After Lung Transplantation Is Associated with Bronchiolitis Obliterans Syndrome Development. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/high-regulatory-t-cells-rate-early-after-lung-transplantation-is-associated-with-bronchiolitis-obliterans-syndrome-development/. Accessed May 9, 2025.« Back to 2017 American Transplant Congress