Session Time: 2:30pm-4:00pm
Presentation Time: 2:54pm-3:06pm
Introduction: There is a need for the development of suitable large animal models for research in gastroenterology and intestinal transplantation. Ex vivo normothermic perfusion (EVNP) is increasingly used for studying isolated organs under controlled perfusion conditions. Here we report, for the first time, a reliable and effective technique for EVNP of segmental porcine small intestine.
Methods: Segments of small intestine (n=4) 1.5-3.0m were retrieved from terminally-anaesthetised pigs following exsanguination and in situ perfusion with cold preservation solution. EVNP was performed after a mean cold ischaemia time of 5h 20min using oxygenated autologous blood diluted with Ringer's solution. EVNP was performed at 37[deg]C with a mean pressure of 80 mmHg for 2h. The duration of EVNP was extended to 4h for the second experiments in which two segments of proximal to mid-ileum (1.5-3.0m) were retrieved from each pig (n=5) and reperfused with whole blood (control) or with leukocyte-depleted blood to examine the impact of leukocyte depletion on reperfusion injury.
Results: All bowel segments were well-perfused, exhibited peristalsis during EVNP. Venous glucose levels significantly increased following luminal glucose stimulation (basal level 1.8±0.6 mmol/L vs. peak 15.5±5.8 mmol/L) and glucagon-like peptide-1 (GLP-1) levels also increased in all experiments, demonstrating intact absorptive and secretory intestinal functions. There were no significant differences between the control group (n=5) and the leukocyte-depleted group (n=5) in blood flow, venous glucose, GLP-1 levels or histopathology at the end of 4h of EVNP.
Conclusion: We provide proof-of-concept evidence for the utility of this novel experimental model for the investigation of intestinal physiology, pathology and ischaemia reperfusion injury, as well as for evaluation of potential therapeutic interventions.
CITATION INFORMATION: Hamed M, Barlow A, Khosla S, Sagar A, Gribble F, Murphy M, Pettigrew G, Bolton E, Bradley J, Nicholson M, Hosgood S, Saeb-Parsy K. Ex Vivo Normothermic Perfusion of Isolated Segmental Porcine Bowel: A Novel Functional Model of the Small Intestine. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Hamed M, Barlow A, Khosla S, Sagar A, Gribble F, Murphy M, Pettigrew G, Bolton E, Bradley J, Nicholson M, Hosgood S, Saeb-Parsy K. Ex Vivo Normothermic Perfusion of Isolated Segmental Porcine Bowel: A Novel Functional Model of the Small Intestine. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/ex-vivo-normothermic-perfusion-of-isolated-segmental-porcine-bowel-a-novel-functional-model-of-the-small-intestine/. Accessed February 23, 2019.
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